乙肝病毒包膜蛋白：整个病毒生命周期中的分子体操

StephenW

The Hepatitis B Virus Envelope Proteins: Molecular Gymnastics Throughout the Viral Life Cycle
Stefan Seitz  1 , Jelena Habjanič  2   3 , Anne K Schütz  2   3 , Ralf Bartenschlager  1   4
Affiliations
Affiliations

    1
    Department of Infectious Diseases, University of Heidelberg, 69120 Heidelberg, Germany; email: [email protected].
    2
    Bavarian NMR Center, Department of Chemistry, Technical University of Munich, 85748 Garching, Germany.
    3
    Institute of Structural Biology, Helmholtz Zentrum München, 85764 Neuherberg, Germany.
    4
    Division of Virus-Associated Carcinogenesis, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.

    PMID: 32600157 DOI: 10.1146/annurev-virology-092818-015508

Abstract

New hepatitis B virions released from infected hepatocytes are the result of an intricate maturation process that starts with the formation of the nucleocapsid providing a confined space where the viral DNA genome is synthesized via reverse transcription. Virion assembly is finalized by the enclosure of the icosahedral nucleocapsid within a heterogeneous envelope. The latter contains integral membrane proteins of three sizes, collectively known as hepatitis B surface antigen, and adopts multiple conformations in the course of the viral life cycle. The nucleocapsid conformation depends on the reverse transcription status of the genome, which in turn controls nucleocapsid interaction with the envelope proteins for virus exit. In addition, after secretion the virions undergo a distinct maturation step during which a topological switch of the large envelope protein confers infectivity. Here we review molecular determinants for envelopment and models that postulate molecular signals encoded in the capsid scaffold conducive or adverse to the recruitment of envelope proteins. Expected final online publication date for the Annual Review of Virology, Volume 7 is September 29, 2020. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

StephenW

乙肝病毒包膜蛋白：整个病毒生命周期中的分子体操
Stefan Seitz 1，JelenaHabjanič2 3，Anne KSchütz2 3，Ralf Bartenschlager 1 4
隶属关系
隶属关系

    1个
    海德堡大学传染病系，德国海德堡69120；电子邮件：[email protected]。
    2
    慕尼黑工业大学化学系，巴伐利亚的NMR中心，德国加辛市85748。
    3
    Helmholtz ZentrumMünchen的结构生物学研究所，德国纽黑尔堡85764。
    4
    德国海德堡69120，德国癌症研究中心（DKFZ）病毒相关癌变研究室。

    PMID：32600157 DOI：10.1146 / annurev-virology-092818-015508

抽象

从受感染的肝细胞释放出的新的乙型肝炎病毒粒子是复杂的成熟过程的结果，该过程始于核衣壳的形成，从而提供了有限的空间，通过逆转录合成病毒DNA基因组。通过在异质包膜中包裹二十面体核衣壳来完成病毒颗粒装配。后者包含三种大小的完整膜蛋白，统称为乙型肝炎表面抗原，并在病毒生命周期中采用多种构象。核衣壳的构象取决于基因组的逆转录状态，这反过来又控制了核衣壳与包膜蛋白的相互作用，从而使病毒退出。另外，在分泌后，病毒体经历了独特的成熟步骤，在此过程中，大包膜蛋白的拓扑转换赋予了感染力。在这里，我们审查了包围的分子决定因素和模型，这些模型假定了衣壳支架中编码的分子信号有利于或不利于包膜蛋白的募集。 《病毒学年度回顾》（第7卷）的最终最终在线发布日期为2020年9月29日。有关修订的估算，请参见http://www.annualreviews.org/page/journal/pubdates。