对青藏高原乙型肝炎病毒的完整基因组分析：HBsAg和抗HBs抗体

StephenW

Complete genome analysis of hepatitis B virus in Qinghai-Tibet plateau: the geographical distribution, genetic diversity, and co-existence of HBsAg and anti-HBs antibodies

    He Liu, Liping Shen, Shuang Zhang, Feng Wang, Guomin Zhang, Zundong Yin, Feng Qiu, Xiaofeng Liang, Fuzhen Wang & Shengli Bi

Virology Journal volume 17, Article number: 75 (2020) Cite this article

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Abstract
Background

The genetic variation and origin of Hepatitis B Virus (HBV) in Qinghai-Tibet Plateau were poorly studied. The coexistence of HBsAg and anti-HBs has been described as a puzzle and has never been reported in the indigenous population or in recombinant HBV sequences. This study aimed to report geographical distribution, genetic variability and seroepidemiology of HBV in southwest China.
Methods

During 2014–2017, 1263 HBsAg positive serum were identified and 183 complete genome sequences were obtained. Serum samples were collected from community-based populations by a multistage random sampling method. Polymerase chain reaction (PCR) was used to amplify the HBV complete genome sequences. Then recombination, genetic variability, and serological analysis were performed.
Results

(1) Of the 1263 HBsAg positive serum samples, there were significant differences between the distribution of seromarkers in Tibet and Qinghai. (2) Of 183 complete genome sequences, there were 130 HBV/CD1 (71.0%), 49 HBV/CD2 (26.8%) and four HBV/C2 isolates (2.2%). Serotype ayw2 (96.1%) was the main serological subtype. (3) Several nucleotide mutations were dramatically different in CD1 and CD2 sequences. Clinical prognosis-related genetic variations such as nucleotide mutation T1762/A1764 (27.93%), A2189C (12.85%), G1613A (8.94%), T1753C (8.38%), T53C (4.47%) T3098C (1.68%) and PreS deletion (2.23%) were detected in CD recombinants. (4) From the inner land of China to the northeast boundary of India, different geographical distributions between CD1 and CD2 were identified. (5) Twenty-seven (2.14%) HBsAg/HBsAb coexistence serum samples were identified. S protein amino acid mutation and PreS deletion were with significant differences between HBsAg/HBsAb coexistence group and control group.
Conclusions

HBV/CD may have a mixed China and South Asia origin. Based on genetic variations, the clinical prognosis of CD recombinant seems more temperate than genotype C strains in China. The HBsAg/HBsAb coexistence is a result of both PreS deletion and aa variation in S protein. Several unique mutations were frequently detected in HBV/CD isolates, which could potentially influence the clinical prognosis.

StephenW

对青藏高原乙型肝炎病毒的完整基因组分析：HBsAg和抗HBs抗体的地理分布，遗传多样性和共存

    刘鹤，沉丽萍，张爽，王峰，张国敏，殷尊东，邱秋，梁晓峰，王福珍＆毕胜利

Virology Journal卷17，文章编号：75（2020）引用本文

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抽象
背景

对青藏高原乙型肝炎病毒（HBV）的遗传变异和起源研究较少。 HBsAg和抗HBs的共存被描述为一个难题，从未在土著人群或重组HBV序列中报道。本研究旨在报告中国西南地区HBV的地理分布，遗传变异和血清流行病学。
方法

在2014–2017年期间，鉴定出了1263 HBsAg阳性血清，并获得了183个完整的基因组序列。通过多阶段随机抽样方法从社区人群中收集血清样本。聚合酶链反应（PCR）用于扩增HBV完整基因组序列。然后进行重组，遗传变异和血清学分析。
结果

（1）在1263例HBsAg阳性血清样本中，西藏和青海的血清标志物分布之间存在显着差异。 （2）在183个完整的基因组序列中，有130个HBV / CD1（71.0％），49个HBV / CD2（26.8％）和4个HBV / C2分离株（2.2％）。血清型ayw2（96.1％）是主要的血清学亚型。 （3）一些核苷酸突变在CD1和CD2序列中有显着差异。临床预后相关的遗传变异，例如核苷酸突变T1762 / A1764（27.93％），A2189C（12.85％），G1613A（8.94％），T1753C（8.38％），T53C（4.47％）T3098C（1.68％）和PreS缺失（在CD重组物中检测到2.23％）。 （4）从中国内陆到印度东北边界，确定了CD1和CD2之间的不同地理分布。 （5）鉴定了二十七（2.14％）个HBsAg / HBsAb共存血清样品。 HBsAg / HBsAb共存组与对照组相比，S蛋白氨基酸突变和PreS缺失有显着差异。
结论

HBV / CD可能来自中国和南亚。根据遗传变异，在中国，CD重组体的临床预后似乎比C基因型菌株更为温和。 HBsAg / HBsAb共存是PreS缺失和S蛋白氨基酸变异的结果。在HBV / CD分离物中经常检测到几个独特的突变，可能会影响临床预后。

StephenW

https://virologyj.biomedcentral. ... /s12985-020-01350-w