在患有慢性HBV感染的人中，粘膜相关的不变T细胞被严重减少

StephenW

Mucosal-associated Invariant T-cells Are Severely Reduced and Exhausted in Humans With Chronic HBV Infection
Wenyong Huang  1   2   3 , Wenjing He  1   2   3 , Xiaomin Shi  1   2   3 , Qianyu Ye  1   2   3 , Xiaoshun He  1   2   3 , Dou Lang  1   2   3 , Yifang Gao  1   2   3
Affiliations
Affiliations

    1
    Organ Transplantation Center, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
    2
    Guangdong Provincial Key Laboratory of Organ Donation and Transplant Immunology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, P. R. China.
    3
    Guangdong Provincial International Cooperation Base of Science and Technology (Organ Transplantation), The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

    PMID: 32510704 DOI: 10.1111/jvh.13341

Abstract

Chronic hepatitis B virus (CHBV) infection is a major cause of liver diseases. Mucosal-associated invariant T (MAIT) cells are important for antiviral immunity in the liver, but the distinction between intrasinusoidal and peripheral MAIT cells in patients with CHBV infections remains unclear. PBMCs were obtained from patients with CHBV infections (n=29) and age-matched controls (n=46). Liver-associated mononuclear cells (LMCs) were collected from healthy donors (n=29) and explanted livers (n=19) from patients and used for phenotypic, functional and TCR diversity analyses. The percentages of both peripheral and intrasinusoidal MAIT cells were significantly reduced in the CHBV infection group compared to the control group. Peripheral MAIT cells from CHBV-infected patients expressed higher levels of HLA-DR, CD69, CD38, and PD-1 than those of controls. We also confirmed that peripheral MAIT cells in HBV patients had elevated expression T-cell exhaustion genes. Except for a difference in the level of PD-1, no differences were observed between the liver MAIT cells of the two groups. The production of IFN-α in peripheral MAIT cells of CHBV infection patients was lower than in control patients, but no such difference was observed in liver MAIT cells. Additionally, a distinct TCR signature was found in CHBV patients. Hence we found distinct activities and functions in liver and peripheral MAIT cells of patients with CHBV infections.

Keywords: CD38; CD69; Chronic hepatitis B virus; HLA-DR; IFN-γ; MAIT cells; PD-1.

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StephenW

在患有慢性HBV感染的人中，粘膜相关的不变T细胞被严重减少和消耗
黄文勇1 2 3，何文静1 2 3，石小敏1 2 3，叶倩玉1 2 3，何小顺1 2 3，豆浪1 2 3，高逸芳1 2 3
隶属关系
隶属关系

    1个
    中山大学附属第一医院器官移植中心，中国广州
    2
    中山大学附属第一医院广东省器官捐赠与移植免疫学重点实验室，中国广州
    3
    中山大学附属第一医院广东省国际科学技术合作基地（器官移植），广州。

    PMID：32510704 DOI：10.1111 / jvh.13341

抽象

慢性乙型肝炎病毒（CHBV）感染是肝脏疾病的主要原因。粘膜相关的不变T（MAIT）细胞对于肝脏中的抗病毒免疫很重要，但是在CHBV感染患者中窦内和外周MAIT细胞之间的区别仍然不清楚。从患有CHBV感染（n = 29）和年龄匹配的对照组（n = 46）的患者中获得PBMC。从患者的健康供体（n = 29）和移植肝脏（n = 19）收集与肝脏相关的单核细胞（LMC），并将其用于表型，功能和TCR多样性分析。与对照组相比，CHBV感染组外周血和窦内MAIT细胞的百分比均显着降低。来自CHBV感染患者的外周MAIT细胞表达的HLA-DR，CD69，CD38和PD-1水平高于对照组。我们还证实，HBV患者的外周MAIT细胞具有升高的表达T细胞衰竭基因。除了PD-1水平的差异外，两组的肝MAIT细胞之间均未观察到差异。 CHBV感染患者外周MAIT细胞中IFN-α的产生低于对照组，但在肝MAIT细胞中未观察到这种差异。另外，在CHBV患者中发现了独特的TCR特征。因此，我们在患有CHBV感染的患者的肝脏和外周MAIT细胞中发现了不同的活动和功能。

关键字：CD38； CD69;慢性乙型肝炎病毒； HLA-DR； IFN-γ； MAIT细胞； PD-1。

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