慢性肝炎患者血清维生素D3浓度与HBV DNA水平和免疫反应之间

StephenW

Nutrients. 2020 Apr 16;12(4). pii: E1114. doi: 10.3390/nu12041114.
The Study of Correlation Between Serum Vitamin D3 Concentrations and HBV DNA Levels and Immune Response in Chronic Hepatitis Patients.
Ko WS1,2,3, Yang YP4,5, Shen FP1, Wu MC5, Shih CJ1, Lu MC2, Yan YH1,2,3, Chiou YL1.
Author information

1
    Department of Nutrition, Master of Biomedical Nutrition Program, Hungkuang University, Taichung 433, Taiwan.
2
    Department of Medicine Research, Kuang-Tien General Hospital, Taichung 433, Taiwan.
3
    Department of Internal Medicine, Kuang-Tien General Hospital, Taichung 433, Taiwan.
4
    Personnel Management Office, Ton-Yen General Hospital, Hsinchu 300, Taiwan.
5
    Department of Health Business Administration, Hungkuang University, Taichung 433, Taiwan.

Abstract

Chronic hepatitis B (CHB) is a common chronic disease. Previous studies have shown a link between 25-hydroxyvitamin D3 (vitamin D3) concentration and liver disease. Hepatitis B virus (HBV) infection has been attributed to the inappropriate functioning of cell-mediated immunity. However, the effects of vitamin D3, immune cell, and HBeAg status on HBV viral load in CHB patients are still unclear. We investigated the relationship between the serum concentration of vitamin D3, percentage of immune cells in peripheral blood, and the HBV viral load of CHB patients. Sixty CHB patients were recruited, and their blood samples were collected and analyzed. Vitamin D level was measured using a chemiluminescence assay. A level of 30 ng/mL or above was defined as a vitamin D3 sufficiency. We assigned vitamin D3 status as either normal (≥30 ng/mL), insufficient (20-30 ng/mL), or deficient (<20 ng/mL). T-lymphocyte and B-lymphocyte surface markers in peripheral blood were detected using flow cytometry. The factors associated with HBV viral load were analyzed using univariate and multivariate-adjusted models. The mean serum vitamin D3 concentration in the subjects was 20.9±5.6 ng/mL. Up to 88.3% of the patients were either deficient in or had insufficient vitamin D3. The gender, BMI, hepatitis B surface antigen levels, and ALT levels were significantly related to serum vitamin D3 levels. Serum vitamin D3 concentration, HBe status, HBs levels, ALT, and AST levels showed a statistically significant correlation with the HBV DNA levels. Serum vitamin D3 concentrations and hepatitis B surface antigen levels were strongly correlated with HBV DNA levels. Vitamin D3 levels were significantly associated with CD19 numbers (β:-6.2, 95% CI: -10.5). In multivariate analysis, vitamin D3 levels in the deficient and insufficient groups, and the CD8, HBeAg, and WBC counts were significantly associated with HBV DNA levels. In the immune tolerance phase of HBeAg-negative chronic HBV infection, vitamin D3 may be a modulator of immune function via CD8, CD19, and HBV DNA.
KEYWORDS:

25-hydroxyvitamin D3; HBV DNA; chronic hepatitis B; lymphocyte surface markers

PMID:
    32316365
DOI:
    10.3390/nu12041114

StephenW

营养物质。 2020年4月16日; 12（4）。 Pii：E1114。 Doi：10.3390 / nu12041114。
慢性肝炎患者血清维生素D3浓度与HBV DNA水平和免疫反应之间的相关性研究。
Ko WS1,2,3，Yang YP4,5，Shen FP1，Wu MC5，Shih CJ1，Lu MC2，Yan YH1,2,3，Chiou YL1。
作者信息

1个
洪光大学营养系，生物医学营养学硕士，台湾台中433。
2
台湾台中433广田总医院医学研究室
3
台湾台中433广田总医院内科
4
台湾新竹市吨仁综合医院人事管理处
5
洪光大学卫生工商管理系，台湾台中433。

抽象

慢性乙型肝炎（CHB）是一种常见的慢性疾病。先前的研究表明25-羟基维生素D3（维生素D3）浓度与肝脏疾病之间存在关联。乙型肝炎病毒（HBV）感染已归因于细胞介导的免疫功能异常。但是，尚不清楚CHB患者中维生素D3，免疫细胞和HBeAg状态对HBV病毒载量的影响。我们调查了血清维生素D3浓度，外周血免疫细胞百分比和CHB患者的HBV病毒载量之间的关系。招募了60名CHB患者，并对其血液样本进行了收集和分析。使用化学发光测定法测量维生素D水平。 30 ng / mL或更高的水平定义为维生素D3充足。我们将维生素D3状态指定为正常（≥30ng / mL），不足（20-30 ng / mL）或不足（<20 ng / mL）。使用流式细胞仪检测外周血中的T淋巴细胞和B淋巴细胞表面标记。使用单变量和多变量调整模型分析了与HBV病毒载量相关的因素。受试者的平均血清维生素D3浓度为20.9±5.6 ng / mL。高达88.3％的患者维生素D3不足或不足。性别，BMI，乙型肝炎表面抗原水平和ALT水平与血清​​维生素D3水平显着相关。血清维生素D3浓度，HBe状况，HBs水平，ALT和AST水平与HBV DNA水平具有统计学意义的相关性。血清维生素D3浓度和乙型肝炎表面抗原水平与HBV DNA水平密切相关。维生素D3水平与CD19值显着相关（β：-6.2，95％CI：-10.5）。在多变量分析中，缺乏和不足组的维生素D3水平以及CD8，HBeAg和WBC计数与HBV DNA水平显着相关。在HBeAg阴性慢性HBV感染的免疫耐受阶段，vit amin D3可能通过CD8，CD19和HBV DNA调节免疫功能。
关键字：

25-羟基维生素D3; HBV DNA；慢性乙型肝炎淋巴细胞表面标志

PMID：
32316365
DOI：
10.3390 / nu12041114

StephenW

https://www.mdpi.com/2072-6643/12/4/1114/pdf

ybdn

记得去年有个帖子里研究口服VD补充剂无效

neilhbver

回复 ybdn 的帖子

这个可以自己测试，吃一段时间d3，去医院测测就知道了

乙肝人1949

称职论文，样本60人，?比方说女子学校调查男女比例，肯定失调，但调查方法可以没有问题，但样本实在太少，估计为职称吧

乙肝人1949

称职论文，样本60人，?比方说女子学校调查男女比例，肯定失调，但调查方法可以没有问题，但样本实在太少，估计为职称吧

ybdn

neilhbver 发表于 2020-4-23 22:44
回复 ybdn 的帖子

这个可以自己测试，吃一段时间d3，去医院测测就知道了

你试过没有？这个多年前论坛讨论过，有不少人吃，好像都没效果

StephenW

回复 ybdn 的帖子

正确，研究不是100％明确。 但是如果您的维生素D水平低，则应该服用维生素D补充剂，或者改变饮食习惯，并在阳光下花费更多时间.

ybdn

StephenW 发表于 2020-4-24 14:07
回复 ybdn 的帖子

正确，研究不是100％明确。 但是如果您的维生素D水平低，则应该服用维生素D补充剂，或者 ...

看了几个统计城市办公室工作的人超过90%都是缺VD的，很少有人达标