HBV逆转录酶区域的突变影响乙型肝炎表面抗原滴度及其与HBV D

StephenW

J Infect Dev Ctries. 2019 Nov 30;13(11):1062-1067. doi: 10.3855/jidc.11447.
Mutations in reverse transcriptase region of HBV affect Hepatitis B surface antigen titers and its correlation with HBV DNA.
Shen H1, Chen C2, Ye C3, Zhang H4, Hang S5, Chen M6, Zhu Z7, Xue Y8, Liu L9.
Author information

1
    Department of Clinical Laboratory, the Third peoples' Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
2
    Department of Clinical Laboratory, the Third peoples' Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
3
    Institute of Hepatology, the Third People's Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
4
    Department of Clinical Laboratory, the Third peoples' Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
5
    Department of Clinical Laboratory, the Third peoples' Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
6
    Department of Clinical Laboratory, the Third peoples' Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
7
    Department of Clinical Laboratory, the Third peoples' Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
8
    Institute of Hepatology, the Third People's Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].
9
    Institute of Hepatology, the Third People's Hospital of Changzhou, Changzhou, Jiangsu, China. [email protected].

Abstract
INTRODUCTION:

The impact of mutations in the reverse transcriptase region of HBV on serum HBsAg titer and its correlation with HBV DNA is largely unknown.
METHODOLOGY:

A total of 644 patients, with a history of lamivudine or adefovir dipivoxil resistance who underwent genotypic resistance tests, were enrolled in this study. Serum HBsAg, hepatitis B e antigen and HBV DNA were quantified, and the HBV RT region was sequenced and analyzed. Then, the patients were divided into five sub-groups, including M204I/V, L180M+M204I/V, A181T/V, N236T and A181T/V+N236T according to the mutation spectra.
RESULTS:

HBsAg was lower in the wild-type and A181T/V+N236T groups as compared to the M204I/V, L180M+M204I/V and N236T groups. HBsAg was positively correlated with HBV DNA levels in the wild-type group (r = 0.322, p < 0.01), as well as in the M204I/V, L180M+M204I/V, A181T/V, and N236T subgroups, while no correlation was found in the A181T/V+N236T subgroup (r = 0.159, p = 0.217). Moreover, for patients with N236T mutation, HBsAg was positively correlated with HBV DNA level in the HBeAg negative group (r = 0.435, p = 0.016), but not in the HBeAg positive group (r = 0.105, p = 0.594). For patients with A181T/V or N236T mutation, HBsAg was positively correlated with HBV DNA in older patients (≥ 40 years), but not in younger patients (< 40 years).
CONCLUSIONS:

Serum HBsAg titer and its correlation with HBV DNA may be affected by mutations in the reverse transcriptase region of HBV, that should be re-evaluated in patients with antiviral resistance.

Copyright (c) 2019 Longgen Liu, hongyu shen, chunhua chen, chunyan ye, hongyu zhang, shuangxiong hang, min chen, zhen zhu, yuan xue.
KEYWORDS:

drug resistance; hepatitis B surface antigen; hepatitis B virus; mutation

PMID:
    32087080
DOI:
    10.3855/jidc.11447

StephenW

J感染开发人员。 2019十一月30; 13（11）：1062-1067。 doi：10.3855 / jidc.11447。
HBV逆转录酶区域的突变影响乙型肝炎表面抗原滴度及其与HBV DNA的相关性。
沉H1，陈C2，叶C3，张H4，杭S5，陈M6，朱Z7，薛Y8，刘L9。
作者信息

1个
    常州市第三人民医院检验科，江苏常州。 [email protected]。
2
    常州市第三人民医院检验科，江苏常州。 [email protected]。
3
    常州市第三人民医院肝病研究所，江苏常州[email protected]。
4
    常州市第三人民医院检验科，江苏常州。 [email protected]。
5
    常州市第三人民医院检验科，江苏常州。 [email protected]。
6
    常州市第三人民医院检验科，江苏常州。 [email protected]。
7
    常州市第三人民医院检验科，江苏常州。 [email protected]。
8
    常州市第三人民医院肝病研究所，江苏常州[email protected]。
9
    常州第三人民医院肝病研究所，江苏常州[email protected]。

抽象
介绍：

HBV逆转录酶区域突变对血清HBsAg滴度的影响及其与HBV DNA的相关性尚不清楚。
方法：

该研究共纳入了644名接受拉米夫定或阿德福韦酯抗性史的患者，并接受了基因型耐药性测试。定量血清HBsAg，乙型肝炎e抗原和HBV DNA，并对HBV RT区域进行测序和分析。然后，根据突变谱将患者分为M204I / V，L180M + M204I / V，A181T / V，N236T和A181T / V + N236T五个亚组。
结果：

与M204I / V，L180M + M204I / V和N236T组相比，野生型和A181T / V + N236T组的HBsAg较低。 HBsAg与野生型组（r = 0.322，p <0.01）以及M204I / V，L180M + M204I / V，A181T / V和N236T亚组中的HBV DNA水平呈正相关，但无相关性在A181T / V + N236T子组中发现（r = 0.159，p = 0.217）。此外，对于具有N236T突变的患者，HBsAg阴性组的HBsAg与HBV DNA水平呈正相关（r = 0.435，p = 0.016），而HBeAg阳性组则与HBV DNA水平无正相关（r = 0.105，p = 0.594）。对于具有A181T / V或N236T突变的患者，年龄较大（≥40岁）的患者中HBsAg与HBV DNA正相关，而年龄较小（<40岁）的患者中HBsAg与HBV DNA正相关。
结论：

血清HBsAg滴度及其与HBV DNA的相关性可能会受到HBV逆转录酶区域突变的影响，对于抗病毒耐药的患者应重新评估。

版权所有（c）2019刘龙根，沉宏宇，陈春华，叶春艳，张宏宇，双雄行，陈敏，朱振，袁学。
关键字：

耐药性;乙肝表面抗原；乙型肝炎病毒；突变

PMID：
    32087080
DOI：
    10.3855 / jidc.11447

StephenW

https://jidc.org/index.php/journal/article/download/11447/2162