根据献血者中的抗HBs携带，隐匿性乙型肝炎病毒感染与包膜

StephenW

Int J Infect Dis. 2019 Dec 23. pii: S1201-9712(19)30499-0. doi: 10.1016/j.ijid.2019.12.026. [Epub ahead of print]
Occurrence of occult hepatitis B virus infection associating with envelope protein mutations according to anti-HBs carriage in blood donors.
Wang J1, Zhang P1, Zeng J2, Du P1, Zheng X2, Ye X2, Zhu W2, Fu Y3, Candotti D4, Allain JP5, Li C6, Li T7.
Author information

1
    Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China.
2
    Shenzhen Blood Center, Shenzhen, China.
3
    Guangzhou Blood Center, Guangzhou, China.
4
    Department of Blood Transmitted Agents, National Institute of Blood Transfusion, Paris, France.
5
    Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China; University of Cambridge, Cambridge, UK.
6
    Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China; School of Public Health, Southern Medical University, Guangzhou, China. Electronic address: [email protected].
7
    Department of Transfusion Medicine, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, China. Electronic address: [email protected].

Abstract
OBJECTIVES:

Occult hepatitis B virus infection (OBI) carries a risk of HBV transmission and hepatocellular carcinoma. With limited sample size, the characteristics of OBI with genotype B or C (OBIB and OBIC) mutations relating to anti-HBs elicited by vaccination or limited host immune response to HBV have not been fully explored.
METHODS:

Occurrence of OBIB or OBIC strains associating with envelope protein (pre-S/S) amino acid substitutions from 99 blood donors stratified according to anti-HBs carriage were extensively characterized.
RESULTS:

According to anti-HBs presence within each genotype, the number or frequency of substitution sites specific from anti-HBs(-) OBIB was higher than those specific of anti-HBs(+) OBIB strains (67 vs 31; 117 vs 41), but the reverse pattern was in OBIC strains (3 vs 24; 3 vs 26). Mutations Pre-s1T68I and sQ129R/L were found uniquely in 15%-25% of anti-HBs(+) OBIB carriers, mutation Pre-s1A54E was found preferentially in anti-HBs(+) OBIC, while seventeen substitutions were found preferentially in 11%-38% of anti-HBs(-) OBIB strains. In the MHR region, mutations sS167 in OBIB, sT118 in OBIC, and sA166 in both genotypes were possibly immune-induced escape mutation sites.
CONCLUSION:

Several mutations in Pre-S/S of OBIs appeared associated with carrier's anti-HBs pressure, which might be risk factors for potential reactivation of viruses under anti-HBs selection in OBI carriers.

Copyright © 2019. Published by Elsevier Ltd.
KEYWORDS:

Anti-HBs; Blood donors; Genotype B or C; Occult HBV; Pre-S/S protein mutations

PMID:
    31877352
DOI:
    10.1016/j.ijid.2019.12.026

StephenW

Int J传染病。 2019年12月23日.pii：S1201-9712（19）30499-0。 doi：10.1016 / j.ijid.2019.12.026。 [Epub提前发布]
根据献血者中的抗HBs携带，隐匿性乙型肝炎病毒感染与包膜蛋白突变相关。
Wang J1，Zhang P1，Zeng J2，Du P1，Zheng X2，Ye X2，Zhu W2，Fu Y3，Candotti D4，Allain JP5，Li C6，Li T7。
作者信息

1个
    南方医科大学检验医学与生物技术学院输血医学系，广州
2
    中国深圳市深圳市血液中心。
3
    中国广州市广州血液中心。
4
    法国巴黎国家输血研究所血液传播媒介系。
5
    南方医科大学检验医学与生物技术学院输血医学系，广州；剑桥大学，英国剑桥。
6
    南方医科大学检验医学与生物技术学院输血医学系，广州；南方医科大学公共卫生学院，广州电子地址：[email protected]。
7
    南方医科大学检验医学与生物技术学院输血医学系，广州电子地址：[email protected]。

抽象
目标：

隐匿性乙型肝炎病毒感染（OBI）具有HBV传播和肝细胞癌的风险。在有限的样本量下，尚未充分探索具有B型或C型基因型（OBIB和OBIC）突变的OBI与疫苗接种或有限的宿主对HBV免疫反应引起的抗HBs相关的特征。
方法：

广泛表征了与根据抗HBs转运分层的来自99名献血者的包膜蛋白（pre-S / S）氨基酸取代相关的OBIB或OBIC菌株的发生。
结果：

根据每种基因型中存在的抗HBs，抗HBs（-）OBIB特异的取代位点的数量或频率要高于抗HBs（+）OBIB菌株的特异性或取代位点（67 vs 31； 117 vs 41），但是相反的模式是OBIC菌株（3对24； 3对26）。在15％-25％的抗HBs（+）OBIB携带者中独特地发现了Pre-s1T68I和sQ129R / L突变，在抗HBs（+）OBIC中优先发现了突变Pre-s1A54E，而在HBIC（+）OBIC中优先发现了17个替代。抗HBs（-）OBIB菌株的11％-38％。在MHR区域，两种基因型的OBIB中的sS167突变，OBIC中的sT118和sA166突变可能是免疫诱导的逃逸突变位点。
结论：

OBIs的Pre-S / S中的几个突变似乎与携带者的抗HBs压力有关，这可能是在OBI携带者选择抗HBs的情况下可能重新激活病毒的危险因素。

版权所有©2019.由Elsevier Ltd发布。
关键字：

抗HBs；献血者；基因型B或C;隐匿性HBV；前S / S蛋白突变

PMID：
    31877352
DOI：
    10.1016 / j.ijid.2019.12.026

StephenW

https://www.ijidonline.com/article/S1201-9712(19)30499-0/pdf