慢性乙型肝炎患者循环调节性T细胞中功能亚群的比例增加

StephenW

Hepatol Res. 2019 Dec 15. doi: 10.1111/hepr.13472. [Epub ahead of print]
Increased proportion of functional subpopulations in circulating regulatory T cells in patients with chronic hepatitis B.
Liu C1, Xu L2, Xia C1, Long Y1, Liu C1, Lu S1, Song Y1.
Author information

1
    Department of Clinical Laboratory, Peking University People's Hospital, Beijing, China.
2
    Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, China.

Abstract
AIM:

This study was designed to investigate the levels of circulating regulatory T (Treg) cells and their functional subpopulations and related cytokines in chronic hepatitis B patients (CHB) and inactive HBsAg carriers.
METHODS:

Peripheral blood of 24 HBV inactive carriers, 26 CHB patients and 34 healthy controls were collected and analyzed by flow cytometry for Treg and CD4+CXCR5+FoxP3+ follicular regulatory T (TFR) cells.IL-10, TGFβ and IL-21 levels in plasma were determined by ELISA. Proportions of functional Treg subpopulations were analyzed by staining of Helios, CD45RA and FoxP3, TIGIT and CD226, and the correlations between Treg subsets and clinical indicators were explored.
RESULTS:

Levels of CD4+FoxP3+ in peripheral blood of CHB patients were significantly increased, and the inhibitory ability of Treg in CHB patients for cytokine secretion was stronger and CD4+CXCR5+FoxP3+ TFR cells were also significantly higher than inactive carriers and healthy controls. TGFβ and IL-10 in plasma of CHB patients were significantly higher than those of healthy controls, with IL-21 levels not significantly changed. Circulating CD4+CXCR5-FoxP3+Treg cells in CHB patients were positively correlated with HBsAg, HBeAg and HBV DNA. The proportions of Helios+FoxP3+, CD45RA-FoxP3hi and CD226-TIGIT+ functional subpopulations in CD4+CXCR5-FoxP3+ Treg cells in CHB patients were significantly increased, and they were significantly correlated with clinical indicators.
CONCLUSIONS:

Circulating Treg cells in CHB patients not only have elevated levels, but their TFR subpopulations are also increased and Treg cells tend to have stronger immunosuppressive functions.

This article is protected by copyright. All rights reserved.
KEYWORDS:

CD226; Helios; TIGIT; chronic hepatitis B; follicular regulatory T cells; regulatory T cells

PMID:
    31840342
DOI:
    10.1111/hepr.13472

StephenW

肝素水库。 2019年12月15日.doi：10.1111 / hepr.13472。 [Epub提前发布]
慢性乙型肝炎患者循环调节性T细胞中功能亚群的比例增加。
刘C1，徐L2，夏C1，龙Y1，刘C1，陆S1，宋Y1。
作者信息

1个
    北京大学人民医院临床实验室，北京。
2
    北京大学健康科学中心，基础医学学院免疫学系，北京。

抽象
目标：

这项研究旨在调查慢性乙型肝炎患者（CHB）和非活性HBsAg携带者中循环调节性T（Treg）细胞的水平及其功能亚群和相关的细胞因子。
方法：

收集24例HBV失活的携带者，26例CHB患者和34例健康对照者的外周血，并通过流式细胞仪分析Treg和CD4 + CXCR5 + FoxP3 +卵泡调节性T（TFR）细胞。血浆中IL-10，TGFβ和IL-21的水平通过ELISA测定。通过Helios，CD45RA和FoxP3，TIGIT和CD226的染色分析功能性Treg亚群的比例，并探讨Treg亚群与临床指标之间的相关性。
结果：

CHB患者外周血CD4 + FoxP3 +水平显着升高，CHB患者Treg对细胞因子分泌的抑制能力更强，CD4 + CXCR5 + FoxP3 + TFR细胞也显着高于无活性的载体和健康对照组。 CHB患者血浆中的TGFβ和IL-10显着高于健康对照者，IL-21水平未见明显变化。 CHB患者循环中的CD4 + CXCR5-FoxP3 + Treg细胞与HBsAg，HBeAg和HBV DNA呈正相关。 CHB患者CD4 + CXCR5-FoxP3 + Treg细胞中Helios + FoxP3 +，CD45RA-FoxP3hi和CD226-TIGIT +功能亚群的比例显着增加，并且与临床指标显着相关。
结论：

CHB患者中的循环Treg细胞不仅水平升高，而且其TFR亚群也增加，并且Treg细胞倾向于具有更强的免疫抑制功能。

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关键字：

CD226;太阳神TIGIT；慢性乙型肝炎卵泡调节性T细胞；调节性T细胞

PMID：
    31840342
DOI：
    10.1111 / hepr.13472