血清pgRNA检测对不同临床结局的HBV感染患者的诊断价值。

StephenW

J Clin Microbiol. 2019 Nov 13. pii: JCM.01275-19. doi: 10.1128/JCM.01275-19. [Epub ahead of print]
Diagnostic value of serum pgRNA detection in HBV-infected patients with different clinical outcomes.
Lin N1,2,3, Ye A1,2,4, Lin J1,2, Liu C1,2, Huang J1,2, Fu Y1,2, Wu S1,2, Xu S1,2, Wang L1,2, Ou Q5,2.
Author information

1
    First Clinical College, Fujian Medical University, Fuzhou, China.
2
    Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
3
    School of Medical Technology and Engineering, Fujian Medical University, Fuzhou, China.
4
    Department of Blood Transfusion, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
5
    First Clinical College, Fujian Medical University, Fuzhou, China [email protected].

Abstract

Pregenomic RNA (pgRNA) is a direct transcription product of HBV covalently closed circular DNA (cccDNA) and plays important roles in viral genome amplification and replication. This study was designed to investigate whether serum pgRNA is a strong alternative marker for reflecting HBV cccDNA levels and to analyze the correlation between serum pgRNA, serum HBV DNA and hepatitis B surface antigen (HBsAg). A total of 400 HBV-infected patients who received nucleos(t)ide analogs (NAs) therapy with different clinical outcomes were involved in this research. Case group included asymptomatic hepatitis B virus carrier (ASC), chronic hepatitis B (CHB), liver cirrhosis (LC), and hepatocellular carcinoma (HCC), 100 patients for each group. The results showed that the level of HBV pgRNA had significant difference in these 4 groups. Serum pgRNA levels correlated well with serum HBV DNA levels and HBsAg levels (HBV pgRNA levels vs. HBV DNA levels, r=0.58, P<0.001; HBV pgRNA levels vs. HBsAg levels, r=0.47, P<0.001). In addition, we focused on the 108 HBV-infected patients with HBV DNA <500 IU/ml, it was surprising to find that 17.57% (13/74) of HBV pgRNA can be detected even when HBV DNA level was below 20 IU/ml. In conclusion, HBV pgRNA levels in serum can be a surrogate marker for intrahepatic HBV cccDNA compared with serum HBV DNA and HBsAg. The detection of serum HBV pgRNA levels may provide a reference for clinical monitoring of cccDNA levels and the selection of appropriate timing for discontinuing antiviral therapy, especially when HBV DNA levels are below the detection limit.

Copyright © 2019 American Society for Microbiology.

PMID:
    31723011
DOI:
    10.1128/JCM.01275-19

StephenW

临床微生物学杂志。 2019年11月13日.pii：JCM.01275-19。 doi：10.1128 / JCM.01275-19。 [Epub提前发布]
血清pgRNA检测对不同临床结局的HBV感染患者的诊断价值。
Lin N1,2,3，Ye A1,2,4，Lin J1,2，Liu C1,2，Huang J1,2，Fu Y1,2，Wu S1,2，Xu S1,2，Wang L1,2，Ou问题5，2。
作者信息

1个
    福建医科大学第一临床学院，福州。
2
    福建医科大学附属第一医院检验医学科，福州。
3
    福建医科大学医学技术与工程学院，福州。
4
    福建医科大学附属第一医院输血科，福州。
5
    福建医科大学第一临床学院，福州[email protected]。

抽象

前基因组RNA（pgRNA）是HBV共价闭合环状DNA（cccDNA）的直接转录产物，在病毒基因组扩增和复制中起重要作用。本研究旨在调查血清pgRNA是否是反映HBV cccDNA水平的强力替代标记，并分析血清pgRNA，血清HBV DNA和乙型肝炎表面抗原（HBsAg）之间的相关性。本研究共纳入400名接受不同临床结果的核苷酸（t）核苷酸类似物（NAs）治疗的HBV感染患者。病例组包括无症状乙型肝炎病毒携带者（ASC），慢性乙型肝炎（CHB），肝硬化（LC）和肝细胞癌（HCC），每组100例。结果表明，这4组患者的HBV pgRNA水平存在显着差异。血清pgRNA水平与血清​​HBV DNA水平和HBsAg水平密切相关（HBV pgRNA水平与HBV DNA水平之间，r = 0.58，P <0.001; HBV pgRNA水平与HBsAg水平之间，r = 0.47，P <0.001）。此外，我们针对108名HBV DNA <500 IU / ml的HBV感染患者进行了研究，令人惊讶的是，即使HBV DNA水平低于20 IU / ml，仍可检测到17.57％（13/74）HBV pgRNA。毫升总之，与血清HBV DNA和HBsAg相比，血清HBV pgRNA水平可以作为肝内HBV cccDNA的替代指标。血清HBV pgRNA水平的检测可为cccDNA水平的临床监测以及选择终止抗病毒治疗的适当时机提供参考，特别是当HBV DNA水平低于检测限时。

版权所有©2019美国微生物学会。

PMID：
    31723011
DOI：
    10.1128 / JCM.01275-19

乙肝人1949

ARC520失败的时候说了一句话，说Pgrna是CCCDNA的唯一表征物。这文章也是这个意思