用HBs和HBc蛋白结合羧基乙烯基聚合物进行鼻内疫苗接种可诱

StephenW

Biochem Biophys Res Commun. 2019 Sep 30. pii: S0006-291X(19)31799-1. doi: 10.1016/j.bbrc.2019.09.072. [Epub ahead of print]
Intranasal vaccination with HBs and HBc protein combined with carboxyl vinyl polymer induces strong neutralizing antibody, anti-HBs IgA, and IFNG response.
Sanada T1, Yamamoto N1, Kayesh MEH2, Tsukiyama-Kohara K2, Hasegawa H3, Miyazaki T4, Takano JI5, Shiogama Y5, Yasutomi Y5, Goh Y6, Yoshida O7, Hiasa Y7, Kohara M8.
Author information

1
    Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.
2
    Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima-city, Kagoshima 890-8580, Japan.
3
    Department of Pathology, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo 162-8640, Japan; Influenza Virus Research Center, National Institute of Infectious Diseases, Gakuen, Musashimurayama-city, Tokyo 208-0011, Japan.
4
    Toko Yakuhin Kogyo Co., Ltd., 20 Tsuji, Tateyama-machi, Toyama, 930-0211, Japan.
5
    Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Health and Nutrition, Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan.
6
    Beacle, Inc., Yoshida-kawaracho, Sakyo-ku, Kyoto, 606-8305, Japan.
7
    Department of Gastroenterology and Metabology, Ehime University Graduate School of Medicine, Shizukawa, Toon, Ehime, 791-0295, Japan.
8
    Department of Microbiology and Cell Biology, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan. Electronic address: [email protected].

Abstract

Hepatitis B virus (HBV) infection causes acute and chronic hepatitis, which is a major public health concern worldwide. Immunization methods incorporating hepatitis B surface-small (HBs-S) antigen and hepatitis B core antigen (HBc) have been proposed as candidate therapeutic vaccines, but the elimination of existing HBV infection remains a challenge. To enhance the efficacy of HBs and HBc vaccination, we investigated HBs-large (HBs-L) as an immunogen, and carboxyl vinyl polymer (CVP) as an excipient. HBs-S or HBs-L, in combination with HBc antigen, was administered subcutaneously (without CVP) or intranasally (with or without CVP) for the evaluation of immune response in the tree shrew, which is considered to be a suitable small animal model of HBV infection. Immunization with HBs-L antigen by either route induced a rapid IgG response. Intranasal immunization with HBs-S or HBs-L and HBc formulated with CVP strongly induced neutralizing antibody activity, IgA response, and HBc-specific expression of the interferon gamma-encoding gene. These data indicated the potential of HBs-L and HBc intranasal immunization with CVP, not only as a therapeutic vaccine, but also as a prophylactic vaccine candidate.

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:

Carboxyl vinyl polymer; HBs-L; Hepatitis B virus; Intranasal administration; Tree shrew; Vaccine

PMID:
    31582218
DOI:
    10.1016/j.bbrc.2019.09.072

StephenW

Biochem Biophys Res Commun。 2019年9月30日。pii：S0006-291X（19）31799-1。 doi：10.1016 / j.bbrc.2019.09.072。 [Epub提前发布]
用HBs和HBc蛋白结合羧基乙烯基聚合物进行鼻内疫苗接种可诱导强烈的中和抗体，抗HBs IgA和IFNG反应。
Sanada T1，Yamamoto N1，Kayesh MEH2，Tsukiyama-Kohara K2，Hasegawa H3，Miyazaki T4，Takano JI5，Shiogama Y5，Yasutomi Y5，Goh Y6，Yoshida O7，Hiasa Y7，Kohara M8。
作者信息

1个
    东京都医学科学研究所微生物与细胞生物学系，东京都世田谷区上北泽市，日本156-8506。
2
    鹿儿岛大学兽医学联合学院，日本鹿儿岛市鹿儿岛市栗本市890-8580。
3
    日本东京都新宿区富山市国立传染病研究所病理学教研室，日本162-8640;国立传染病研究所流行性感冒病毒研究中心，武藏村山市学园，日本东京208-0011。
4
    日本Toyama Yakuhin Kogyo Co.，Ltd.富山市立山町Tsuji 20，930-0211，Japan。
5
    国家生物医学创新，健康与营养研究所筑波灵长类动物研究中心，茨城筑波八dai平，日本茨城县305-0843。
6
    日本606-8305，日本京都市左京区吉田川原町，Beacle公司。
7
    爱媛大学医学院大学院胃肠病学和代谢学系，爱媛县香川县香川县香椿，791-0295。
8
    东京都医学科学研究所微生物与细胞生物学系，东京都世田谷区上北泽市，日本156-8506。电子地址：[email protected]。

抽象

乙型肝炎病毒（HBV）感染会导致急性和慢性肝炎，这是全球主要的公共卫生问题。已经提出了结合乙肝表面小抗原（HBs-S）和乙肝核心抗原（HBc）的免疫方法作为候选治疗疫苗，但是如何消除现有的HBV感染仍然是一个挑战。为了提高HBs和HBc疫苗接种的功效，我们研究了HBs-large（HBs-L）作为一种免疫原，以及羧基乙烯基聚合物（CVP）作为一种赋形剂。 HBs-S或HBs-L与HBc抗原联合皮下注射（无CVP）或鼻内注射（有CVP或无CVP），以评估树sh的免疫反应，这被认为是合适的小动物模型乙肝病毒感染。通过任一途径用HBs-L抗原免疫均可引起快速的IgG反应。用CVP配制的HBs-S或HBs-L和HBc进行鼻内免疫可强烈诱导中和抗体活性，IgA反应和干扰素γ编码基因的HBc特异性表达。这些数据表明用CVP进行HBs-L和HBc鼻内免疫的潜力，不仅可以作为治疗性疫苗，而且可以作为预防性疫苗的候选者。

版权所有©2019 The Authors。由Elsevier Inc.出版。保留所有权利。
关键字：

羧基乙烯基聚合物；乙肝病毒乙型肝炎病毒；鼻内给药；树sh疫苗

PMID：
    31582218
DOI：
    10.1016 / j.bbrc.2019.09.072

乙肝人1949

古巴药升级版?，为毛非要很鼻腔干什么劲

dxd2017

回复 乙肝人1949 的帖子

可能鼻子内的皮肤和其他地方的皮肤不一样，好吸收，避免了注射