联合检测AFP-L3，GP73和TIP30可提高HBV相关肝硬化和肝细胞癌的

StephenW

J Pak Med Assoc. 2019 Sep;69(9):1279-1286.
Combined detection of AFP-L3, GP73 and TIP30 enhances diagnostic accuracy for HBV-related cirrhosis and hepatocellular carcinoma.
Yanming L1, Yue C2, Wencan C1, Liangyin WU1, Xijun L1, Jiafeng Z3, Fengping HE1.
Author information

1
    Department of Clinical Laboratory, Yuebei People's Hospital, Shaoguan, Guangdong, P.R. China.
2
    Department of Medical Technology, Medical College of Shaoguan University, Shaoguan, Guandong P.R. China.
3
    Department of Hepatobiliary Surgery, Yuebei People's Hospital, Shaoguan, Guangdong, P.R. China.

Abstract
OBJECTIVE:

To investigate the value of combined tests of serum Golgi protein-73, alpha-fetoprotein- L3 and Tat-interacting protein-30 in the diagnosis of hepatitis B virus related cirrhosis and hepatocellular carcinoma.
METHODS:

The cross-sectional study was conducted at Yuebei People's Hospital, Guangdong, China, from January to October 2017, and comprised hepatitis B patients and healthy controls. Serum Golgi protein-73, alpha-fetoprotein-L3 and Tat-interacting protein-30 levels in both groups were detected by enzyme-linked immunosorbent assay (ELISA). Alpha-fetoprotein-L3 was separated and quantified by electrochemiluminescence immunoas says and the percentage of alpha-fetoprotein-L3 to alphafetoprotein was calculated.
RESULTS:

Of the 721 subjects, 525(%) were patients and 196(%) were healthy controls. Among the patients, 271(%) had chronic hepatitis B, 161(%) had liver cirrhosis and 93(%) had hepatocellular carcinoma. Serum Golgi protein-73, alpha-fetoprotein-L3 and Tat-interacting protein-30 levels were significantly different in the hepatocellular carcinoma patients compared to controls, and those with chronic hepatitis and liver cirrhosis (p<0.01 each). The sensitivity and specificity of the combined detection of the three serum levels for diagnosing cirrhosis were 78.26% and 86.72%. The corresponding values for diagnosing hepatocellular carcinoma were 86.02% and 92.51%.
CONCLUSIONS:

Combined detection of Golgi protein-73, alpha fetoprotein-L3 and Tat-interacting protein was found to have the potential to improve diagnostic accuracy.
KEYWORDS:

Hepatocellular carcinoma, Cirrhosis, Chronic hepatitis B, Tumour marker

PMID:
    31511712

StephenW

J Pak Med Assoc。 2019年9月; 69（9）：1279-1286。
联合检测AFP-L3，GP73和TIP30可提高HBV相关肝硬化和肝细胞癌的诊断准确性。
阎明L1，岳C2，文灿C1，吴良银1，西军L1，嘉丰Z3，冯平和1。
作者信息

1
广东省韶关市粤北人民医院检验科，中国。
2
韶关学院医学院医学技术系，广东韶关，中国。
3
广东省韶关市粤北人民医院肝胆外科，中国。

抽象
目的：

目的探讨血清Golgi蛋白-73，甲胎蛋白-L3和Tat相互作用蛋白-30联合检测对乙型肝炎病毒相关性肝硬化和肝细胞癌的诊断价值。
方法：

横断面研究于2017年1月至10月在中国广东省粤北人民医院进行，烧伤乙型肝炎患者和健康对照。血清高尔基体蛋白-73，甲胎蛋白-L3和Tat相互作用蛋白-30水平在两组中通过酶联免疫吸附测定（ELISA）检测。通过电化学发光免疫法分离并定量甲胎蛋白-L3，并计算甲胎蛋白-L3对甲胎蛋白的百分比。
结果：

在721名受试者中，525名（％）是患者，196名（％）是健康对照。在这些患者中，271（％）患有慢性乙型肝炎，161（％）患有肝硬化，93（％）患有肝细胞癌。与对照组和慢性肝炎和肝硬化患者相比，肝细胞癌患者的血清高尔基体蛋白-73，甲胎蛋白-L3和Tat相互作用蛋白-30水平显着不同（各自p <0.01）。联合检测三种血清水平诊断肝硬化的敏感性和特异性分别为78.26％和86.72％。诊断肝细胞癌的相应值分别为86.02％和92.51％。
结论：

发现高尔基体蛋白-73，甲胎蛋白-L3和Tat相互作用蛋白的组合检测具有提高诊断准确性的潜力。
关键词：

肝细胞癌，肝硬化，慢性乙型肝炎，肿瘤标志物

结论：
31511712

StephenW

https://jpma.org.pk/article-details/9310?article_id=9310