葡聚糖颗粒是HBsAg的强效佐剂，有利于抗病毒免疫。

StephenW

Mol Pharm. 2019 Apr 9. doi: 10.1021/acs.molpharmaceut.8b01322. [Epub ahead of print]
Glucan particles are a powerful adjuvant for the HBsAg, favoring antiviral immunity.
Soares E, Groothuismink ZM, Boonstra A, Borges O.
Abstract

The lack of vaccine adjuvants that are able to induce robust T cell responses fosters the search for more powerful options. Pathogen-like particles are a promising approach. The adjuvant activity of pathogen-like particles is highly influenced by size and surface composition. This study aimed to evaluate the adjuvant potential of two different β-glucan-based particles: blend chitosan/β-glucan particles (ChiGluPs), which are positively charged and have mean size of 1276 nm, and neutral yeast-derived glucan particles (GPs) with a mean size of 3 µm. Additionally, chitosan particles (ChiPs) were used to understand the effect of β-glucan addition (ChiGluPs). Mouse spleen cells responded through the production of either TNF-α or RANTES, following in vitro stimulation with particles containing either β-glucan (ChiGluPs and GPs) or chitosan (ChiGluPs and ChiPs). Human monocytes responded to all particles through TNF-α secretion. Subcutaneous vaccination of mice with the hepatitis B surface antigen (HBsAg) showed increased serum IgG for all particles compared to HBsAg alone (435-fold, 4500-fold or 2500-fold increase for either ChiPs, ChiGluPs or GPs). Interestingly, only GPs elicited the secretion of HBsAg-specific Th1, Th2, Th9, Th17, Th22 and Treg related cytokines. This study demonstrates, for the first time, that GPs can have a significant role against the hepatitis B virus, by favoring antiviral immunity.

PMID:
    30964694
DOI:
    10.1021/acs.molpharmaceut.8b01322

StephenW

Mol Pharm。 2019年4月9日doi：10.1021 / acs.molpharmaceut.8b01322。 [印刷前的电子版]
葡聚糖颗粒是HBsAg的强效佐剂，有利于抗病毒免疫。
Soares E，Groothuismink ZM，Boonstra A，Borges O.
抽象

缺乏能够诱导强大T细胞反应的疫苗佐剂促使人们寻求更强大的选择。病原体样颗粒是一种很有前途的方法。病原体样颗粒的佐剂活性受尺寸和表面组成的影响很大。这项研究旨在评估两种不同的β-葡聚糖基颗粒的辅助潜力：混合壳聚糖/β-葡聚糖颗粒（ChiGluPs），其带正电荷，平均大小为1276 nm，和中性酵母衍生的葡聚糖颗粒（GPs） ）平均尺寸为3微米。另外，壳聚糖颗粒（ChiP）用于理解β-葡聚糖添加的效果（ChiGluP）。在用含有β-葡聚糖（ChiGluPs和GP）或壳聚糖（ChiGluP和ChiP）的颗粒进行体外刺激后，小鼠脾细胞通过产生TNF-α或RANTES而产生应答。人单核细胞通过TNF-α分泌对所有颗粒起反应。与单独的HBsAg相比，用乙型肝炎表面抗原（HBsAg）对小鼠进行皮下接种显示所有颗粒的血清IgG增加（对于ChiP，ChiGluP或GPs，增加435倍，4500倍或2500倍）。有趣的是，只有GPs引起HBsAg特异性Th1，Th2，Th9，Th17，Th22和Treg相关细胞因子的分泌。这项研究首次表明，通过支持抗病毒免疫，全科医生可以对乙型肝炎病毒发挥重要作用。

结论：
    30964694
DOI：
    10.1021 / acs.molpharmaceut.8b01322

newchinabok

治疗性疫苗是可以成功的，只不过还没找到好的路径

齐欢畅

fuke

回复 newchinabok 的帖子

现在对病毒的研究越来越深入了。离最终治愈应该不远了。希望十年内可以彻底攻克乙肝。

左罗

回复 fuke 的帖子

10年太久远，3－5年应该是可以实现的吧

newchinabok

左罗 发表于 2019-4-12 11:58
回复 fuke 的帖子

10年太久远，3－5年应该是可以实现的吧

3—5年太久远，rep早就攻克了