可通过STING配体上调对HBV疫苗的体液和细胞免疫应答的诱导。

StephenW

Virology. 2019 Mar 21;531:233-239. doi: 10.1016/j.virol.2019.03.013. [Epub ahead of print]
Induction of humoral and cellular immune response to HBV vaccine can be up-regulated by STING ligand.
Ito H1, Kanbe A2, Hara A3, Ishikawa T4.
Author information

1
    Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan. Electronic address: [email protected].
2
    Department of Informative Clinical Medicine, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
3
    Department of Tumor Pathology, Gifu University Graduate School of Medicine, 1-1 Yanagido, Gifu 501-1194, Japan.
4
    Department of Medical Technology, Nagoya University School of Health Sciences, 1-20 Daikominami-1-chome, Higashi-ku, Nagoya, Aichi 461-8673, Japan.

Abstract

A persistent hepatitis B virus (HBV) infection is characterized by a lack of or a weak immune response to HBV. Efficient induction of the HBV-specific immune response leads to the clearance of HBV. Stimulator of interferon (IFN) genes (STING) is a cytoplasmic sensor of intracellular DNA from microbes and host cells. In the present study, we examined the efficacy of cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) that is a ligand of the STING pathway as an HBV vaccine adjuvant. Wild-type (WT) mice and HBV-transgenic (HBV-Tg) mice were immunized with hepatitis B surface antigen (HBsAg) and cGAMP. The vaccination with HBsAg and cGAMP significantly enhanced the humoral and cellular immune response to HBsAg in WT and HBV-Tg mice. Cytokine production related to Th1 and Th2 responses and the activation of antigen-presenting cells in lymphoid tissues were induced by cGAMP. Vaccination using cGAMP may overcome tolerance in patients with chronic HBV infection.

Copyright © 2019. Published by Elsevier Inc.
KEYWORDS:

Hepatitis B virus; Immune response; STING; Vaccination

PMID:
    30928701
DOI:
    10.1016/j.virol.2019.03.013

StephenW

病毒学。 2019年3月21日; 531：233-239。 doi：10.1016 / j.virol.2019.03.013。 [印刷前的电子版]
可通过STING配体上调对HBV疫苗的体液和细胞免疫应答的诱导。
Ito H1，Kanbe A2，Hara A3，Ishikawa T4。
作者信息

1
    岐阜大学医学研究科信息临床医学系，日本岐阜市柳田市1-1-1 501-1194。电子地址：[email protected]。
2
    岐阜大学医学研究科信息临床医学系，日本岐阜市柳田市1-1-1 501-1194。
3
    岐阜大学大学院医学研究科肿瘤病理科，日本岐阜县柳田市1-1-1 501-1194。
4
    名古屋大学健康科学学院医疗技术系，日本爱知县名古屋市东区大二一丁目1-20号461-8673。

抽象

持续性乙型肝炎病毒（HBV）感染的特征在于对HBV缺乏或弱的免疫应答。有效诱导HBV特异性免疫应答导致HBV清除。干扰素（IFN）基因的刺激物（STING）是来自微生物和宿主细胞的细胞内DNA的细胞质传感器。在本研究中，我们检测了作为STING途径的配体的环鸟苷一磷酸 - 腺苷一磷酸（cGAMP）作为HBV疫苗佐剂的功效。用乙型肝炎表面抗原（HBsAg）和cGAMP免疫野生型（WT）小鼠和HBV-转基因（HBV-Tg）小鼠。 HBsAg和cGAMP的疫苗接种显着增强了WT和HBV-Tg小鼠对HBsAg的体液和细胞免疫应答。通过cGAMP诱导与Th1和Th2应答相关的细胞因子产生和淋巴组织中抗原呈递细胞的活化。使用cGAMP进行疫苗接种可以克服慢性HBV感染患者的耐受性。

版权所有©2019。Elsevier Inc.出版
关键词：

乙型肝炎病毒;免疫反应;刺;疫苗接种

结论：
    30928701
DOI：
    10.1016 / j.virol.2019.03.013