Toll样受体，长非编码RNA NEAT1和RIG-I表达与活动期HBeAg阳性慢性

StephenW

J Clin Lab Anal. 2019 Mar 29:e22886. doi: 10.1002/jcla.22886. [Epub ahead of print]
Toll-like receptors, long non-coding RNA NEAT1, and RIG-I expression are associated with HBeAg-positive chronic hepatitis B patients in the active phase.
Zeng Y1,2, Wu W1,2, Fu Y1,2, Chen S1,2, Chen T1,2, Yang B1,2, Ou Q1,2.
Author information

1
    Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
2
    Department of Gene Diagnosis, Fujian Medical University, Fuzhou, China.

Abstract
BACKGROUND:

Innate immunity plays a crucial role in host-virus interactions and greatly influences viral replication including HBV infection. However, few studies have investigated the possible antiviral immune roles played by TLRs, RIG-I, and long no-coding RNA NEAT1 in chronic HBV infection (CHB) patients in clinical samples and their relationships among immune responses. In this study, we sought to investigate the mRNA expression levels of TLR1-10, RIG-I, and NEAT1 expression in HBeAg-positive CHB treatment-naïve patients with the active phase.
METHODS:

The expression levels of TLR1-10, RIG-I, and NEAT1 of CHB patients with the active phase and healthy controls were measured by qPCR. Serum HBV DNA and routine liver biochemistry including ALT, etc were also measured to evaluate the impaired physiological function of the liver affected by CHB.
RESULTS:

The expression levels of TLR1 and TLR6 in CHB with active phase were remarkably lower than that in healthy controls. The levels of TLR3 in CHB patients with active phase were remarkably higher than that in healthy controls. The total NEAT1 expression was abnormally decreased in CHB patients as compared with healthy controls. The levels of RIG-I were significantly decreased in CHB patients in the active phase when compared to healthy controls. The expression of TLR6 and RIG-I was closely correlated with NEAT1 expression. TLR6 level was positively correlated with RIG-I level.
CONCLUSION:

Chronic HBV infection can alter the innate immune response by downregulating functional expression of TLR1, TLR6, NEAT1.

© 2019 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc.
KEYWORDS:

active phase; chronic hepatitis B; nuclear-enriched abundant transcript 1; retinoic acid-inducible gene I; toll-like receptor

PMID:
    30924966
DOI:
    10.1002/jcla.22886

StephenW

J Clin Lab Anal。 2019年3月29日：e22886。 doi：10.1002 / jcla.22886。 [印刷前的电子版]
Toll样受体，长非编码RNA NEAT1和RIG-I表达与活动期HBeAg阳性慢性乙型肝炎患者相关。
曾Y1,2，吴W1,2，傅Y1,2，陈S1,2，陈T1,2，杨B1,2，欧Q1,2。
作者信息

1
    福建医科大学附属第一医院检验科，福州
2
    福建医科大学基因诊断系，福州

抽象
背景：

先天免疫在宿主 - 病毒相互作用中起着至关重要的作用，并且极大地影响病毒复制，包括HBV感染。然而，很少有研究调查TLRs，RIG-I和长期无编码RNA NEAT1在临床样本中的慢性HBV感染（CHB）患者及其免疫应答之间的关系中可能发挥的抗病毒免疫作用。在这项研究中，我们试图调查HBeAg阳性CHB治疗初生患者活动期TLR1-10，RIG-I和NEAT1表达的mRNA表达水平。
方法：

通过qPCR测量具有活性期和健康对照的CHB患者的TLR1-10，RIG-1和NEAT1的表达水平。还测量血清HBV DNA和常规肝脏生化（包括ALT等）以评估受CHB影响的肝脏受损生理功能。
结果：

具有活性期的CHB中TLR1和TLR6的表达水平显着低于健康对照。 CHB患者活动期TLR3水平明显高于健康对照组。与健康对照相比，CHB患者的总NEAT1表达异常降低。与健康对照相比，活跃期CHB患者的RIG-I水平显着降低。 TLR6和RIG-I的表达与NEAT1表达密切相关。 TLR6水平与RIG-I水平呈正相关。
结论：

慢性HBV感染可通过下调TLR1，TLR6，NEAT1的功能性表达来改变先天免疫应答。

©2019作者。 Wiley Periodicals，Inc。出版的临床实验室分析杂志
关键词：

活跃期;慢性乙型肝炎;富含核的丰富转录本1;视黄酸诱导基因I; Toll样受体

结论：
    30924966
DOI：
    10.1002 / jcla.22886