结合预测和术后筛查结果估算中国献血者乙型肝炎阳性率。

StephenW

Transfusion. 2019 Feb 13. doi: 10.1111/trf.15158. [Epub ahead of print]
Estimation of hepatitis B-positive rates in Chinese blood donors by combining predonation and postdonation screening results.
Gao Z1, Liu J2, Fu P1, Huang M3, Cao R3, Wen X3, Zhang C4, He T4, Mao W4, Liao D5, Ke L1, Yang Y1, He M1, Liu Y1, Wang J1, Dodd RY6, Ness P2, Shan H7.
Author information

1
    Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, China.
2
    Johns Hopkins School of Medicine, Baltimore, Maryland.
3
    Mianyang Blood Center, Mianyang, China.
4
    Chongqing Blood Center, Chongqing, China.
5
    RTI International, Rockville, Maryland.
6
    American Red Cross Holland Laboratory, Rockville, Maryland.
7
    Stanford University Medical Center, Stanford, California.

Abstract
BACKGROUND:

Chinese blood centers use Hepatitis B surface antigen (HBsAg) rapid test (RT) in pre-donation and two rounds of screening with different enzyme-linked immunosorbent assays in post-donation. Nucleic acid testing (NAT) on screening non-reactive (SC-) donations has been gradually implemented since 2010. Yet RT+ and SC-/NAT+ donors are seldom included in hepatitis B virus (HBV) positive rate estimates in Chinese blood donors.
METHODS:

We performed HBsAg neutralization test (NT) on whole blood (WB) with pre-donation RT+ results and post-donation screening reactive (SC+) samples from Mianyang and Chongqing in 2015. The annual totals of pre- and post-donation NT+ donors were combined with the annual totals of SC-/NAT+ donors to derive the estimated HBV positive rates.
RESULT:

In Mianyang and Chongqing, 59.4% and 68.2% of RT+ donors in Jan-Aug 2015 contributed for NT, 95.5% and 97.2% of which were NT+ respectively. In 2015, 422 and 667 donors from Mianyang and Chongqing respectively were HBsAg RT+, yielding estimated 403 and 648 pre-donation RT+/NT+ deferrals. 411 and 668 post-donation SC+ samples were NT tested from Mianyang and Chongqing, of which 249 and 323 were NT+ respectively. An estimated 63 donors in Mianyang and 88 donors in Chongqing were SC-/NAT+. The estimated HBV confirmed positive rate in blood donors are 1.59% in Mianyang and 1.01% in Chongqing.
CONCLUSION:

Pre-donation HBsAg RT effectively intercepts donations from HBV infected donors. Using NT confirmatory results from RT+, SC+ and SC-/NAT+ donors, this study provides a model for more accurate estimation for HBV positive rates in China.

© 2019 AABB.

PMID:
    30758046
DOI:
    10.1111/trf.15158

StephenW

输血。 2019年2月13日.doi：10.1111 / trf.15158。 [印刷前的电子版]
结合预测和术后筛查结果估算中国献血者乙型肝炎阳性率。
Gao Z1，Liu J2，Fu P1，Huang M3，Cao R3，Wen X3，Zhang C4，He T4，Mao W4，Liao D5，Ke L1，Yang Y1，He M1，Liu Y1，Wang J1，Dodd RY6，Ness P2 ，Shan H7。
作者信息

1
    中国医学科学院输血研究所，成都。
2
    约翰斯霍普金斯医学院，马里兰州巴尔的摩市。
3
    绵阳血液中心，绵阳，中国。
4
    重庆市血液中心，重庆，中国。
五
    RTI国际，罗克维尔，马里兰州。
6
    美国红十字会荷兰实验室，罗克维尔，马里兰州。
7
    斯坦福大学医学中心，斯坦福，加利福尼亚。

抽象
背景：

中国血液中心在捐献前使用乙型肝炎表面抗原（HBsAg）快速检测（RT），并在捐赠后使用不同的酶联免疫吸附试验进行两轮筛查。自2010年以来，逐步实施了筛选非反应性（SC-）捐赠的核酸检测（NAT）。然而，RT +和SC- / NAT +供体很少被纳入中国献血者的乙型肝炎病毒（HBV）阳性率估计中。
方法：

我们对2015年绵阳和重庆的捐献前RT +结果和捐献后筛查反应性（SC +）样本进行了全血（WB）HBsAg中和试验（NT）。捐献前和捐献后NT +捐献者的年度总数为结合SC- / NAT +捐献者的年度总数，得出估计的HBV阳性率。
结果：

在绵阳和重庆，2015年1月至8月的RT +捐赠者中有59.4％和68.2％为新台币贡献，其中95.5％和97.2％分别为新台币+。 2015年，来自绵阳和重庆的422和667名捐赠者分别为HBsAg RT +，估计有403和648名预捐赠RT + / NT +延期。来自绵阳和重庆的411和668个捐献后SC +样本进行了NT测试，其中249和323分别为NT +。据估计，绵阳的63名捐助者和重庆的88名捐助者为SC- / NAT +。估计HBV确诊献血者的阳性率在绵阳为1.59％，在重庆为1.01％。
结论：

预捐赠HBsAg RT有效拦截HBV感染捐献者的捐赠。利用RT +，SC +和SC- / NAT +供体的NT验证结果，本研究为中国HBV阳性率的更准确估计提供了一个模型。

©2019 AABB。

结论：
    30758046
DOI：
    10.1111 / trf.15158