增加的miR-155水平与慢性乙型肝炎患者外周血中较高的T细胞活

StephenW

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Genet Test Mol Biomarkers. 2019 Feb;23(2):118-123. doi: 10.1089/gtmb.2018.0092.
Increased Levels of miR-155 are Related to Higher T-Cell Activation in the Peripheral Blood of Patients with Chronic Hepatitis B.
Fang J1, Zhuge L2, Rao H3, Huang S2, Jin L2, Li J2.
Author information

1
    1 Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
2
    2 Department of Infectious Diseases, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.
3
    3 Department of Nursing, School of Medicine, Quzhou College of Technology, Quzhou China.

Abstract
OBJECTIVES:

MicroRNA-155 (miR-155) is an important regulator of immune responses in humans. However, its role in T-cell activation in hepatitis B virus (HBV) infection remains unclear.
MATERIALS AND METHODS:

Eighty-one patients with chronic hepatitis B (CHB), 77 HBV carriers, and 51 healthy controls were recruited. HBV DNA and serologic tests were carried out for each subject. Levels of miR-155 in peripheral blood were detected by quantitative reverse transcription/polymerase chain reaction. Immune activation of T-cells was determined by detection of surface molecules CD38 and HLA-DR using flow cytometry.
RESULTS:

We found higher miR-155 levels in CD4+ and CD8+ T-cells of CHB patients than HBV carriers or healthy controls (p < 0.01), moreover, miR-155 levels in the CD8+ T-cells of HBV carriers were higher than in healthy controls (p < 0.01). Furthermore, immune activation of CD4+ and CD8+ T-cells in CHB patients was much higher than in healthy controls (p < 0.01).
CONCLUSION:

Our findings suggest that miR-155 expression positively correlates with T-cell activation, especially in CHB patients, and is a potential biomarker for immune activation and disease progression in HBV infection.
KEYWORDS:

CD38; HLA-DR; chronic hepatitis B; hepatitis B virus; immune activation; microRNA-155

PMID:
    30735455
DOI:
    10.1089/gtmb.2018.0092

StephenW

Genet Test Mol Biomarkers。 2019年2月; 23（2）：118-123。 doi：10.1089 / gtmb.2018.0092。
增加的miR-155水平与慢性乙型肝炎患者外周血中较高的T细胞活化有关。
方J1，诸葛L2，饶H3，黄S2，金L2，李J2。
作者信息

1
    1浙江大学医学院附属第一医院泌尿外科，杭州
2
    2温州医科大学附属第二医院感染科和温英儿童医院，温州，中国。
3
    3衢州工学院医学院护理系，中国衢州。

抽象
目的：

MicroRNA-155（miR-155）是人类免疫反应的重要调节因子。然而，其在乙型肝炎病毒（HBV）感染中T细胞活化中的作用仍不清楚。
材料和方法：

招募了81名患有慢性乙型肝炎（CHB），77名HBV携带者和51名健康对照的患者。对每个受试者进行HBV DNA和血清学测试。通过定量逆转录/聚合酶链反应检测外周血中miR-155的水平。通过使用流式细胞术检测表面分子CD38和HLA-DR来确定T细胞的免疫活化。
结果：

我们发现CHB患者CD4 +和CD8 + T细胞中miR-155水平高于HBV携带者或健康对照组（p <0.01），而且HBV携带者CD8 + T细胞中miR-155水平高于健康对照组。 （p <0.01）。此外，CHB患者中CD4 +和CD8 + T细胞的免疫激活远高于健康对照（p <0.01）。
结论：

我们的研究结果表明miR-155表达与T细胞活化正相关，特别是在CHB患者中，并且是HBV感染中免疫激活和疾病进展的潜在生物标志物。
关键词：

CD38; HLA-DR;慢性乙型肝炎;乙型肝炎病毒;免疫激活;微小RNA-155

结论：
    30735455
DOI：
    10.1089 / gtmb.2018.0092