芪术汤提取物通过抑制NF-κB信号通路在体外和C57BL / 6小鼠中

StephenW

Sci Rep. 2019 Feb 5;9(1):1415. doi: 10.1038/s41598-018-38391-9.
Extracts of Qizhu decoction inhibit hepatitis and hepatocellular carcinoma in vitro and in C57BL/6 mice by suppressing NF-κB signaling.
Wan LF1, Shen JJ2, Wang YH2, Zhao W3, Fang NY1, Yuan X4, Xue BY5,6.
Author information

1
    Department of Infectious Disease, Affiliated Hospital of Nanjing University of Chinese Medicine, 155 HanZhong Road, Nanjing, 210029, Jiangsu, China.
2
    Department of Laboratory, Affiliated Hospital of Nanjing University of Chinese Medicine, 155 HanZhong Road, Nanjing, 210029, Jiangsu, China.
3
    School of Laboratory Medicine, Chengdu medical college, 783 Xindu Road, Chengdu, 610500, Sichuan, China.
4
    The First Clinical Medical College, Nanjing University of Chinese Medicine, 155 HanZhong Road, Nanjing, 210029, Jiangsu, China.
5
    Department of Infectious Disease, Affiliated Hospital of Nanjing University of Chinese Medicine, 155 HanZhong Road, Nanjing, 210029, Jiangsu, China. [email protected].
6
    The First Clinical Medical College, Nanjing University of Chinese Medicine, 155 HanZhong Road, Nanjing, 210029, Jiangsu, China. [email protected].

Abstract

Hepatitis and hepatocellular carcinoma are serious human diseases. Here, we examined the in vivo and in vitro inhibitory effect of extracts of Qizhu decoction (a traditional Chinese medicine) on hepatitis caused by diethylnitrosamine or hepatitis B virus and on diethylnitrosamine-induced hepatocellular carcinoma. The results showed that both the aqueous and ethanol extracts (QC and QS, respectively) of Qizhu decoction significantly inhibited hepatic inflammation and liver cancer induced by diethylnitrosamine or hepatitis B virus by suppressing NF-κB signaling and decreasing the levels of TNF-α and IL-1β. Both QC and QS inhibited the proliferation and migration of primary cancer hepatocytes by reducing cyclin B1, cyclin D1 and N-cadherin expression and increasing E-cadherin expression. QC and QS also promoted the apoptosis of primary cancer hepatocytes by upregulating caspase-3 and downregulating BCL-2 expression. The knockdown of p65 in NF-κB signaling inhibited the ability of QC and QS to significantly reduce the colony formation ability of liver cancer cells. Additionally, QC and QS might significantly inhibit the DNA replication of hepatitis B virus in vivo and in vitro, and we found that corilagin and polydatin were the active compounds of QC and QS. Taken together, our in vitro findings and our results in C57BL/6 mice showed that extracts of Qizhu decoction might inhibit hepatitis and hepatocellular carcinoma by suppressing NF-κB signaling.

PMID:
    30723284
DOI:
    10.1038/s41598-018-38391-9

StephenW

Sci Rep.20119 Feb 5; 9（1）：1415。 doi：10.1038 / s41598-018-38391-9。
芪术汤提取物通过抑制NF-κB信号通路在体外和C57BL / 6小鼠中抑制肝炎和肝细胞癌。
Wan LF1，Shen JJ2，Wang YH2，Zhao W3，Fang NY1，Yuan X4，Xue BY5,6。
作者信息

1
    南京中医药大学附属医院感染科，江苏南京汉中路155号，江苏210029
2
    南京中医药大学附属医院实验室，江苏南京汉中路155号，江苏210029
3
    成都医学院检验医学院，成都市新都路783号，四川610500
4
    南京中医药大学第一临床医学院，南京汉中路155号，江苏210029
五
    南京中医药大学附属医院感染科，江苏南京汉中路155号，江苏210029 [email protected]。
6
    南京中医药大学第一临床医学院，南京汉中路155号，江苏210029 [email protected]。

抽象

肝炎和肝细胞癌是严重的人类疾病。在这里，我们检测了芪术汤（一种中药）提取物对二乙基亚硝胺或乙型肝炎病毒引起的肝炎和二乙基亚硝胺诱导的肝细胞癌的体内和体外抑制作用。结果表明，芪术汤水溶液和乙醇提取物（QC和QS）均能通过抑制NF-κB信号，降低TNF-α和IL水平，显着抑制二乙基亚硝胺或乙型肝炎病毒引起的肝脏炎症和肝癌。 -1β。 QC和QS均通过减少细胞周期蛋白B1，细胞周期蛋白D1和N-钙粘蛋白表达以及增加E-钙粘蛋白表达来抑制原代癌肝细胞的增殖和迁移。 QC和QS还通过上调caspase-3和下调BCL-2表达促进原发性肝癌细胞的凋亡。 NF-κB信号传导中p65的敲低抑制了QC和QS显着降低肝癌细胞集落形成能力的能力。此外，QC和QS可能在体内和体外显着抑制乙型肝炎病毒的DNA复制，我们发现corilagin和白藜芦醇苷是QC和QS的活性化合物。综上所述，我们的体外研究结果和我们在C57BL / 6小鼠中的结果表明，杞术汤提取物可能通过抑制NF-κB信号传导来抑制肝炎和肝细胞癌。

结论：
    30723284
DOI：
    10.1038 / s41598-018-38391-9

StephenW

https://www.nature.com/articles/s41598-018-38391-9

hao2014

不得了

中医又出神药了