可通过HBV总RNA评估怀孕的HBV患者

StephenW

Hannah Dellabella
February 05, 2019
Pregnant Patients With HBV May Be Assessed via HBV Total RNA


Researchers found that serum HBV total RNA correlates better with other established markers than HBV pgRNA in pregnant HBV carriers. Researchers found that serum HBV total RNA correlates better with other established markers than HBV pgRNA in pregnant HBV carriers.

In pregnant patients with chronic hepatitis B, hepatitis B virus (HBV) total RNA may help monitor liver disease and viral replication during pregnancy, according to results published in the Journal of Clinical Virology.

The study included pregnant and postpartum participants with chronic hepatitis B (n=46). The researchers collected serum and plasma from each participant as well as clinical data on demographics, HBV e antigen (HBeAg) status, quantitative hepatitis B s antigen (qHBsAg) levels, HBV DNA, alanine aminotransferase (ALT), postpartum liver stiffness measurement, and treatment regimen. The researchers used in-house assays to quantify serum HBV total RNA and pre-genomic RNA. They used direct population sequencing of HBV surface gene to determine HBV genotype.


The results indicated that HBV DNA, qHBsAg, and ALT levels were comparable between participants who were pregnant and those who were post-partum. Participants who were pregnant had higher serum HBV total RNA levels and lower serum HBV pre-genomic RNA levels compared with those who were postpartum.

The researchers found that HBeAg status correlated with HBV DNA, serum HBV total RNA, and qHBsAg levels during pregnancy. For postpartum participants, the researchers found that HBeAg status correlated with HBV DNA and serum HBV total RNA.

The results indicated that serum HBV total RNA levels correlated with HBeAg status, HBV DNA, qHBsAg, ALT, and liver stiffness measurement, whereas serum HBV pre-genomic RNA did not (P <.05).

“This suggests that serum HBV total RNA has the potential to serve as a complementary HBV biomarker to monitor and assess HBV replication and liver disease in pregnant [chronic hepatitis B] carriers,” the researchers concluded.

Reference

Patel NH, Joshi SS, Lau KCK, Castillo E, Coffin CS. Analysis of serum hepatitis B virus RNA levels in a multiethnic cohort of pregnant chronic hepatitis B carriers. J Clin Virol. 2019;111:42-47.

StephenW

汉娜德拉贝拉
2019年2月5日
可通过HBV总RNA评估怀孕的HBV患者


研究人员发现血清HBV总RNA与怀孕HBV携带者中HBV pgRNA的其他既定标志物相关性更好。研究人员发现血清HBV总RNA与怀孕HBV携带者中HBV pgRNA的其他既定标志物相关性更好。

根据发表在“临床病毒学杂志”上的研究结果，在怀孕的慢性乙型肝炎患者中，乙型肝炎病毒（HBV）总RNA可能有助于监测怀孕期间的肝脏疾病和病毒复制。

该研究包括患有慢性乙型肝炎的孕妇和产后参与者（n = 46）。研究人员收集了每位参与者的血清和血浆，以及人口统计学，HBV e抗原（HBeAg）状态，定量乙型肝炎抗原（qHBsAg）水平，HBV DNA，丙氨酸氨基转移酶（ALT），产后肝硬度测量和治疗方案。研究人员使用内部分析来量化血清HBV总RNA和前基因组RNA。他们使用HBV表面基因的直接人口测序来确定HBV基因型。


结果表明，怀孕的参与者和产后的HBV DNA，qHBsAg和ALT水平相当。与产后相比，怀孕的参与者具有更高的血清HBV总RNA水平和更低的血清HBV前基因组RNA水平。

研究人员发现，HBeAg状态与妊娠期间HBV DNA，血清HBV总RNA和qHBsAg水平相关。对于产后参与者，研究人员发现HBeAg状态与HBV DNA和血清HBV总RNA相关。

结果表明，血清HBV总RNA水平与HBeAg状态，HBV DNA，qHBsAg，ALT和肝硬度测量相关，而血清HBV前基因组RNA则没有（P <.05）。

“这表明血清HBV总RNA有可能作为一种互补的HBV生物标志物来监测和评估怀孕[慢性乙型肝炎]携带者的HBV复制和肝脏疾病，”研究人员总结道。

参考

Patel NH，Joshi SS，Lau KCK，Castillo E，Coffin CS。 妊娠慢性乙型肝炎患者多民族队列血清乙型肝炎病毒RNA水平分析。 J Clin Virol。2019;111：42-47。