脾脏相关性对乙型肝炎病毒相关性肝硬化门静脉高压症免疫

StephenW

Journal of Interferon & Cytokine ResearchVol. 39, No. 2 Research Reports
Spleen-Associated Effects on Immunity in Hepatitis B Virus-Related Cirrhosis with Portal Hypertension
Na Huang
, Fan Pu Ji
, Shu Zhang
, Zhenzhen Li
, Jun Li
, Rui Zhou
, Shaoying Zhang
, Wei Wei
, Liang Li
, Haiyan Chen
, Baohua Li
, Guangyao Kong
, Jun Yang
, and Zongfang Li
Published Online:5 Feb 2019https://doi.org/10.1089/jir.2018.0121

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Abstract

Our study aimed to investigate the histologic and immunological changes of portal hypertension (PH) pre- and postsplenectomy in hepatitis B virus (HBV)-related cirrhosis. Peripheral blood samples were obtained from 30 patients with HBV-related cirrhosis and PH at pre- and postsplenectomy time points and from 15 healthy subjects. Spleen tissue specimens were collected from 15 of the patients with HBV-related cirrhosis and from 8 control patients who had undergone splenectomy due to trauma. Immunohistochemical staining was performed to evaluate the immune effector cells and the expression of negative immune regulators. Flow cytometry was used to investigate the immunophenotypes and percentages. The spleen of cirrhotic patients with PH showed extensive depletion of splenic CD4, CD8, and human leukocyte antigen DR cells along with overexpression of the inhibitory receptors programmed death-1 (PD-1) and T cell immunoglobulin domain and mucin domain-3 and their ligands (PD-L2 and galectin-9). Peripheral blood of patients with PH showed remarkable decrease in proportions of CD8 T cell and natural killer (NK) cells and increase in regulatory T (Treg) cells, as well as high expression of PD-1 in CD4/8 T cells. Compared with presplenectomy patients, cirrhotic patients with PH showed increased proportions of CD8 and NK cells, decreased proportion of Treg cells, and decreased expression of PD-1 in peripheral blood CD4/8 T cells after splenectomy. PH-spleen could lead to peripheral tolerance and immunosuppression in HBV cirrhotic patients, and splenectomy may cause beneficial immunological changes.

StephenW

干扰素与细胞因子研究杂志Vol. 39，第2号研究报告
脾脏相关性对乙型肝炎病毒相关性肝硬化门静脉高压症免疫功能的影响
娜黄
，范普吉
，舒舒
，珍珍李
，李军
，周瑞
，张少英
，魏伟
，梁莉
，陈海燕
，李宝华
，光耀孔
杨军
和李宗芳
在线发布：2019年2月5日https：//doi.org/10.1089/jir.2018.0121

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抽象

我们的研究旨在调查乙型肝炎病毒（HBV）相关性肝硬化门静脉高压症（PH）前后和脾切除术的组织学和免疫学变化。外周血样品来自30名HBV相关性肝硬化患者和脾切除术前和术后时间点以及15名健康受试者的PH。从15名患有HBV相关性肝硬化的患者和8名因创伤而接受脾切除术的对照患者中收集脾组织标本。进行免疫组织化学染色以评估免疫效应细胞和负免疫调节剂的表达。流式细胞术用于研究免疫表型和百分比。患有PH的肝硬化患者的脾脏显示脾脏CD4，CD8和人白细胞抗原DR细胞的广泛消耗，以及抑制性受体程序性死亡-1（PD-1）和T细胞免疫球蛋白结构域和粘蛋白结构域-3及其过表达的过度表达。配体（PD-L2和半乳糖凝集素-9）。患有PH的患者的外周血显示CD8T细胞和自然杀伤（NK）细胞的比例显着降低，并且调节性T（Treg）细胞增加，以及CD4 / 8T细胞中PD-1的高表达。与脾切除术患者相比，脾切除术后患有PH的肝硬化患者CD8和NK细胞比例增加，Treg细胞比例减少，外周血CD4 / 8T细胞中PD-1表达减少。 PH-spleen可导致HBV肝硬化患者的外周耐受和免疫抑制，脾切除术可引起有益的免疫学变化。