来自HBV基因组深度测序的见解 - 独特，微小和误解

StephenW

Insights From Deep Sequencing of the HBV Genome—Unique, Tiny, and Misunderstood
Anna L. McNaughton
, Valentina D’Arienzo
, M. Azim Ansari
, Sheila F. Lumley
, Margaret Littlejohn
, Peter Revill
, Jane A. McKeating
, Philippa C. Matthews'Correspondence information about the author Philippa C. MatthewsEmail the author Philippa C. Matthews
Open Access
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DOI: https://doi.org/10.1053/j.gastro.2018.07.058 |


Hepatitis B virus (HBV) is a unique, tiny, partially double-stranded, reverse-transcribing DNA virus with proteins encoded by multiple overlapping reading frames. The substitution rate is surprisingly high for a DNA virus, but lower than that of other reverse transcribing organisms. More than 260 million people worldwide have chronic HBV infection, which causes 0.8 million deaths a year. Because of the high burden of disease, international health agencies have set the goal of eliminating HBV infection by 2030. Nonetheless, the intriguing HBV genome has not been well characterized. We summarize data on the HBV genome structure and replication cycle, explain and quantify diversity within and among infected individuals, and discuss advances that can be offered by application of next-generation sequencing technology. In-depth HBV genome analyses could increase our understanding of disease pathogenesis and allow us to better predict patient outcomes, optimize treatment, and develop new therapeutics.
Keywords:
Hepatitis B Virus, Genotype, Diversity, Evolution
Abbreviations used in the paper:
cccDNA (covalently closed circular DNA), dsDNA (double-stranded DNA), HBsAg (hepatitis B surface antigen), HBeAg (hepatitis B e antigen), HBV (hepatitis B virus), HCC (hepatocellular carcinoma), HIV (human immunodeficiency virus), NGS (next-generation sequencing), ORF (open reading frame), P (reverse transcriptase polymerase), RC-DNA (relaxed circular DNA), RT (reverse transcriptase), S (surface)

StephenW

来自HBV基因组深度测序的见解 - 独特，微小和误解
Anna L. McNaughton
，Valentina D'Arienzo
，M。Azim Ansari
，希拉F.拉姆利
，玛格丽特Littlejohn
，彼得雷维尔
，Jane A. McKeating
，Philippa C. Matthews'关于作者的相关信息Philippa C. Matthews电子邮件作者Philippa C. Matthews
开放存取
PlumX度量标准
DOI：https：//doi.org/10.1053/j.gastro.2018.07.058 |


乙型肝炎病毒（HBV）是一种独特的，微小的，部分双链的逆转录DNA病毒，其蛋白质由多个重叠的阅读框架编码。 DNA病毒的取代率令人惊讶地高，但低于其他逆转录生物的取代率。全世界有超过2.6亿人患有慢性HBV感染，每年导致80万人死亡。由于疾病的高负担，国际卫生机构已经设定了到2030年消除HBV感染的目标。尽管如此，有趣的HBV基因组还没有得到很好的表征。我们总结了HBV基因组结构和复制周期的数据，解释和量化受感染个体内部和之间的多样性，并讨论了应用下一代测序技术可以提供的进展。深入的HBV基因组分析可以增加我们对疾病发病机制的理解，使我们能够更好地预测患者的预后，优化治疗，并开发新的治疗方法。
关键词：
乙型肝炎病毒，基因型，多样性，进化
本文中使用的缩写：
cccDNA（共价闭合环状DNA），dsDNA（双链DNA），HBsAg（乙型肝炎表面抗原），HBeAg（乙型肝炎e抗原），HBV（乙型肝炎病毒），HCC（肝细胞癌），HIV（人类免疫缺陷病毒） ），NGS（新一代测序），ORF（开放阅读框），P（逆转录酶聚合酶），RC-DNA（松弛环状DNA），RT（逆转录酶），S（表面）

StephenW

https://www.gastrojournal.org/ar ... 18)35076-5/abstract