TIM-3作为活动性慢性乙型肝炎患者CD8 + T细胞衰竭的标志物。

StephenW

Microb Pathog. 2019 Jan 17. pii: S0882-4010(18)31275-0. doi: 10.1016/j.micpath.2019.01.026. [Epub ahead of print]
TIM-3 as a marker of exhaustion in CD8+ T cells of active chronic hepatitis B patients.
Mohammadizad H1, Shahbazi M1, Hassanjani-Roshan MR2, Soltanzadeh-Yamchi M1, Mohammadnia-Afrouzi M3.
Author information

1
    Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Department of Immunology, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
2
    Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
3
    Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Immunoregulation Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran; Department of Immunology, School of Medicine, Babol University of Medical Sciences, Babol, Iran. Electronic address: [email protected].

Abstract
BACKGROUND:

Chronic HBV infection presents weak or no virus-specific T-cell responses, implying to an exhausted phenotype, characterized by overexpression of several inhibitory receptors. In the present study, it was aimed to characterize the panel of inhibitory molecules on the CD8+ T cells in patients with active chronic HBV infection.
METHODS:

In this study, 31 active and 32 inactive individuals with chronic HBV infection were recruited. Peripheral blood mononuclear cells were isolated and a multicolor flow cytometry was applied to evaluate the surface inhibitory molecules of TIM3, PD-1, and CD39.
RESULTS:

CD8+ T cells expressing TIM3 were significantly higher in cases with active chronic HBV infection compared to inactive chronic HBV group (8.43 ± 1.4 vs. 5.15 ± 1.43; P < 0.0001). CD8+TIM3+PD-1+ T cells were significantly higher in active chronic HBV cases in comparison to the inactive chronic HBV subjects (4.26 ± 1.04 vs. 3.41 ± 0.74; P < 0.001). Different subpopulations of the CD8+ T cells were correlated with the duration of infection and HBV DNA load in the cases with active chronic HBV infection.
CONCLUSION:

It appears that CD8+ TIM3+ T cells are the major exhausted phenotype of T cells during the active state of HBV infection.

Copyright © 2019. Published by Elsevier Ltd.
KEYWORDS:

CD39; Flow cytometry; HBV; PD-1; TIM3

PMID:
    30660734
DOI:
    10.1016/j.micpath.2019.01.026

StephenW

Microb Pathog。 2019年1月17日.pii：S0882-4010（18）31275-0。 doi：10.1016 / j.micpath.2019.01.026。 [印刷前的电子版]
TIM-3作为活动性慢性乙型肝炎患者CD8 + T细胞衰竭的标志物。
Mohammadizad H1，Shahbazi M1，Hassanjani-Roshan MR2，Soltanzadeh-Yamchi M1，Mohammadnia-Afrouzi M3。
作者信息

1
    巴布尔医科大学健康研究所细胞与分子生物学研究中心，伊朗巴布尔;伊朗巴布尔巴布尔医科大学卫生研究所免疫调节研究中心;巴布尔医科大学医学院免疫学系，伊朗巴布尔。
2
    巴布尔医科大学传染病和热带医学研究中心，伊朗巴布尔。
3
    巴布尔医科大学健康研究所细胞与分子生物学研究中心，伊朗巴布尔;伊朗巴布尔巴布尔医科大学卫生研究所免疫调节研究中心;巴布尔医科大学医学院免疫学系，伊朗巴布尔。电子地址：[email protected]。

抽象
背景：

慢性HBV感染表现出弱或没有病毒特异性T细胞反应，暗示用尽表型，其特征在于几种抑制性受体的过表达。在本研究中，其目的是表征活动性慢性HBV感染患者中CD8 + T细胞上的抑制分子组。
方法：

在这项研究中，招募了31名患有慢性HBV感染的活动和32名非活动个体。分离外周血单核细胞，并应用多色流式细胞仪评估TIM3，PD-1和CD39的表面抑制分子。
结果：

与无活动的慢性HBV组相比，表达TIM3的CD8 + T细胞在活动性慢性HBV感染的情况下显着更高（8.43±1.4对5.15±1.43; P <0.0001）。与无活动的慢性HBV受试者相比，活动性慢性HBV病例中CD8 + TIM3 + PD-1 + T细胞显着升高（4.26±1.04对3.41±0.74; P <0.001）。在活动性慢性HBV感染的病例中，不同亚群的CD8 + T细胞与感染持续时间和HBV DNA载量相关。
结论：

似乎CD8 + TIM3 + T细胞是HBV感染活跃期间T细胞的主要耗竭表型。

版权所有©2019。由Elsevier Ltd.出版
关键词：

CD39;流式细胞仪; HBV; PD-1; TIM3

结论：
    30660734
DOI：
    10.1016 / j.micpath.2019.01.026