血清HBcrAg在反映肝内共价闭合环状DNA方面优于HBV RNA和HBsAg。

StephenW

J Viral Hepat. 2019 Jan 10. doi: 10.1111/jvh.13061. [Epub ahead of print]
Serum HBcrAg is better than HBV RNA and HBsAg in reflecting intrahepatic covalently closed circular DNA.
Chen EQ1,2, Wang ML1,2, Tao YC1,2, Wu DB1,2, Liao J1,2, He M1,2, Tang H1,2.
Author information

1
    Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, PR China.
2
    Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, Sichuan, 610041, PR China.

Abstract

The correlation between serum HBcrAg and HBV RNA is unclear; and correlations of intrahepatic cccDNA with HBcrAg, HBV RNA and HBsAg are rarely reported in the same cohort. This study aimed to assess the correlation of HBcrAg with HBV RNA and HBsAg, and investigate whether serum HBcrAg is superior to serum HBV RNA and HBsAg in reflecting intrahepatic HBV cccDNA in HBeAg-positive and HBeAg-negative CHB patients. In this study, 85 HBeAg-positive and 25 HBeAg-negative patients who have never received antiviral therapy, were included. Among HBeAg-positive patients, HBcrAg was correlated positively with HBsAg(r=0.564, P<0.001) and HBV RNA(r=0.445, P<0.001), and HBV RNA was also correlated positively with HBsAg(r=0.323, P=0.003). Among HBeAg-negative patients, no significantly correlation was observed between HBcrAg, HBsAg and HBV RNA. By multivariable linear regression, HBcrAg(β=-0.563, P<0.001), HBsAg(β=-0.328, P<0.001), and HBV RNA(β =0.180, P=0.003) were all associated with cccDNA levels among HBeAg-positive patients, but only serum HBcrAg was associated with cccDNA level(β=0.774, P=0.000) among HBeAg-negative patients. HBcrAg was better correlated with cccDNA as compared to HBsAg and HBV RNA, irrespective of HBeAg status. Among HBeAg-positive patients, though HBcrAg level was influenced by hepatic inflammatory activity and HBV DNA levels, the good correlations of HBcrAg with cccDNA persisted after stratification by inflammatory activity and HBV DNA levels. In conclusion, correlations of serum HBcrAg, HBV RNA and HBsAg levels differ significantly between HBeAg-positive and HBeAg-negative patients, but serum HbcrAg correlates with cccDNA levels better than HBV RNA and HBsAg, irrespective of HBeAg status. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.
KEYWORDS:

HBV RNA ; covalently closed circular DNA; hepatitis B core-related antigen; hepatitis B surface antigen

PMID:
    30632235
DOI:
    10.1111/jvh.13061

StephenW

J病毒肝病。 2019年1月10日doi：10.1111 / jvh.13061。 [提前打印]
血清HBcrAg在反映肝内共价闭合环状DNA方面优于HBV RNA和HBsAg。
陈EQ1,2，王ML1,2，陶YC1,2，吴DB1,2，廖J1,2，何M1,2，唐H1,2。
作者信息

1
    四川大学华西医院传染病研究中心，四川成都610041
2
    四川大学生物治疗国家重点实验室传染病研究室，四川成都610041

抽象

血清HBcrAg与HBV RNA的相关性尚不清楚;并且在同一队列中很少报道肝内cccDNA与HBcrAg，HBV RNA和HBsAg的相关性。本研究旨在评估HBcrAg与HBV RNA和HBsAg的相关性，探讨HBeAg阳性和HBeAg阴性CHB患者血清HBcrAg是否优于血清HBV RNA和HBsAg反映肝内HBV cccDNA。在这项研究中，纳入了85例HBeAg阳性和25例HBeAg阴性患者，这些患者从未接受过抗病毒治疗。在HBeAg阳性患者中，HBcrAg与HBsAg（r = 0.564，P <0.001）和HBV RNA（r = 0.445，P <0.001）呈正相关，HBV RNA与HBsAg呈正相关（r = 0.323，P = 0.003）。在HBeAg阴性患者中，HBcrAg，HBsAg和HBV RNA之间没有显着相关性。通过多变量线性回归分析，HBcrAg（β= -0.563，P <0.001），HBsAg（β= -0.328，P <0.001）和HBV RNA（β= 0.180，P = 0.003）均与HBeAg-中的cccDNA水平相关。阳性患者，但HBeAg阴性患者血清HBcrAg仅与cccDNA水平相关（β= 0.774，P = 0.000）。与HBsAg和HBV RNA相比，HBcrAg与cccDNA的相关性更好，与HBeAg状态无关。在HBeAg阳性患者中，尽管HBcrAg水平受肝脏炎症活动和HBV DNA水平的影响，但HBcrAg与cccDNA的良好相关性在炎症活性和HBV DNA水平分层后仍然存在。总之，血清HBcrAg，HBV RNA和HBsAg水平的相关性在HBeAg阳性和HBeAg阴性患者之间存在显着差异，但血清HbcrAg与cccDNA水平的相关性优于HBV RNA和HBsAg，与HBeAg状态无关。本文受版权保护。版权所有。

本文受版权保护。版权所有。
关键词：

HBV RNA;共价闭合环状DNA;乙型肝炎核心相关抗原;乙型肝炎表面抗原

结论：
    30632235
DOI：
    10.1111 / jvh.13061