肿瘤DNA依赖性蛋白激酶催化亚基表达与肝细胞癌患者的乙型

StephenW

Sci Rep. 2018 Oct 9;8(1):15019. doi: 10.1038/s41598-018-33427-6.
Tumor DNA-dependent protein kinase catalytic subunit expression is associated with hepatitis B surface antigen status and tumor progression in patients with hepatocellular carcinoma.
Shimizu T1, Aoki T2, Mori S1, Iso Y1, Kato M1, Ishizuka M1, Kubota K1.
Author information

1
    Second Department of Surgery, Dokkyo Medical University, Tochigi, Japan.
2
    Second Department of Surgery, Dokkyo Medical University, Tochigi, Japan. [email protected].

Abstract

The DNA-dependent protein kinase catalytic subunit (DNA-PKcs), which plays an important role in the DNA damage response, has been reported to be associated with tumor progression in various carcinomas. However, the clinical significance of DNA-PKcs in hepatocellular carcinoma (HCC) patients remains unclear. In the present study, we determined the tumor expression of DNA-PKcs in 104 resected HCC specimens by immunohistochemistry. The DNA-PKcs expression was scored as follows; 0, negative staining; 1, staining of nuclei at the tumor edge; 2, staining of the nuclei deep within the tumor and/or the tumor cytoplasm. The relationships between tumor expression of DNA-PKcs and the clinical characteristics and patient outcomes were investigated. Among the 104 HCCs, the distribution of staining for DNA-PKcs was as follows: 32 tumors were assigned a score of 0, 27 tumors were assigned a score of 1, and 45 tumors were assigned a score of 2. Statistical analyses revealed that tumor DNA-PKcs expression was significantly associated with the HBs antigen (HBsAg) status, presence/absence of portal vein invasion, size of the largest tumor nodule (<3.0 cm/>3.0, cm), and the serum alpha-fetoprotein level. Significant differences in the overall survival and recurrence-free survival were observed between patients showing (staining score 1 or 2) and not showing (staining score 0) tumor DNA-PKcs expression (P = 0.049 and P = 0.045, respectively). Our results suggest that tumor expression of DNA-PKcs is associated with tumor progression, HBsAg status and the postoperative outcomes in patients with HCC.

PMID:
    30301934
DOI:
    10.1038/s41598-018-33427-6

StephenW

Sci Rep.20188 Oct 9; 8（1）：15019。 doi：10.1038 / s41598-018-33427-6。
肿瘤DNA依赖性蛋白激酶催化亚基表达与肝细胞癌患者的乙型肝炎表面抗原状态和肿瘤进展相关。
Shimizu T1，Aoki T2，Mori S1，Iso Y1，Kato M1，Ishizuka M1，Kubota K1。
作者信息

1
    日本栃木县Dokkyo医科大学第二外科。
2
    日本栃木县Dokkyo医科大学第二外科。 [email protected]。

抽象

据报道，DNA依赖性蛋白激酶催化亚基（DNA-PKcs）在DNA损伤反应中起重要作用，与各种癌症的肿瘤进展有关。然而，DNA-PKcs在肝细胞癌（HCC）患者中的临床意义仍不清楚。在本研究中，我们通过免疫组织化学确定了104例切除的HCC标本中DNA-PKcs的肿瘤表达。 DNA-PKcs表达评分如下; 0，负染色; 1，肿瘤边缘的细胞核染色; 2，肿瘤深部和/或肿瘤细胞质中的细胞核染色。研究了DNA-PKcs的肿瘤表达与临床特征和患者结果之间的关系。在104例HCC中，DNA-PKcs染色分布如下：32例肿瘤评分为0,27例肿瘤评分为1分，45例肿瘤评分为2.统计分析显示肿瘤DNA-PKcs表达与HBs抗原（HBsAg）状态，门静脉侵入的存在/不存在，最大肿瘤结节的大小（<3.0cm /> 3.0，cm）和血清甲胎蛋白水平显着相关。在显示（染色评分1或2）和未显示（染色评分0）肿瘤DNA-PKcs表达（分别为P = 0.049和P = 0.045）的患者之间观察到总体存活和无复发存活的显着差异。我们的结果表明，DNA-PKcs的肿瘤表达与HCC患者的肿瘤进展，HBsAg状态和术后结果相关。

结论：
    30301934
DOI：

StephenW

https://www.nature.com/articles/s41598-018-33427-6