具有低或不可检测的HBV血浆病毒血症的慢性感染患者的血浆

StephenW

New Microbiol. 2018 Sep 25;41(4). [Epub ahead of print]
Distribution of different HBV DNA forms in plasma and peripheral blood mononuclear cells (PBMCs) of chronically infected patients with low or undetectable HBV plasma viremia.
Vicenti I1, Rossetti B2,3, Mariano S2, Saladini F1, Montagnani F1,2, Zazzi M1, De Luca A2.
Author information

1
    Medical Biotechnology Department, University of Siena, Siena.
2
    UOC Malattie Infettive, Dipartimento di Medicina Interna e Specialistica, AOU Senese.
3
    Clinic of Infectious Diseases, Catholic University of the Sacred Heart, Rome.

Abstract

Few studies have documented hepatitis B virus (HBV) DNA in peripheral blood mononuclear cells (PBMCs). We developed real-time PCR methods for differential amplification of covalently closed circular (cccDNA) and total HBV DNA (tDNA). The different distribution of cccDNA and tDNA in plasma and PBMCs was evaluated in 37 patients with low or undetectable viremia. Plasma tDNA measured by the Abbott reference system and the in-house assay correlated well (Spearman rho = 0.804; P<0.0001). tDNA was detected in four PBMC samples, all from patients with detectable plasma viremia (range 633-6,406 IU/ml), cccDNA was not detected in any sample. The reasons for apparently discrepant results need further investigation but possibly include the high diversification of HBV status and plasma viremia levels.
KEYWORDS:

HBV; PBMC; Real time PCR; cccDNA

PMID:
    30252927

StephenW

新的微生物。 2018年9月25日; 41（4）。 [提前打印]
具有低或不可检测的HBV血浆病毒血症的慢性感染患者的血浆和外周血单核细胞（PBMC）中不同HBV DNA形式的分布。
Vicenti I1，Rossetti B2,3，Mariano S2，Saladini F1，Montagnani F1,2，Zazzi M1，De Luca A2。
作者信息

1
    锡耶纳锡耶纳大学医学生物技术系。
2
    UOC Malattie Infettive，Dipartimento di Medicina Interna e Specialistica，AOU Senese。
3
    罗马圣地天主教大学传染病诊所。

抽象

很少有研究记录外周血单核细胞（PBMCs）中的乙型肝炎病毒（HBV）DNA。我们开发了实时PCR方法，用于差异扩增共价闭合环（cccDNA）和总HBV DNA（tDNA）。在37名低病毒或不可检测的病毒血症患者中评估了血浆和PBMC中cccDNA和tDNA的不同分布。通过Abbott参考系统和内部测定法测量的血浆tDNA相关性良好（Spearman rho = 0.804; P <0.0001）。在4个PBMC样品中检测到tDNA，均来自具有可检测的血浆病毒血症（范围633-6,406IU / ml）的患者，在任何样品中均未检测到cccDNA。结果明显不一致的原因需要进一步调查，但可能包括HBV状态和血浆病毒血症水平的高度多样化。
关键词：

HBV; PBMC;实时PCR; cccDNA的

结论：
    30252927