中国东部军营HBV感染和低水平HBsAg的流行病学调查。

StephenW

Medicine (Baltimore). 2018 Sep;97(38):e12201. doi: 10.1097/MD.0000000000012201.
An epidemiological survey of HBV infection and low-level HBsAg in military camps in eastern China.
Wang T1,2, Dai Y2, Lu W2, Zhou H2, Chen Y3, Xu X4, Sun C2, Cheng J2.
Author information

1
    Faculty of Graduate Studies, Bengbu Medical College, Bengbu.
2
    Department of Clinical Laboratory, The 117th Hospital of PLA.
3
    State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
4
    Department of Biotechnology, The University of Tokyo, Tokyo, Japan.

Abstract

This study aimed to investigate hepatitis B virus (HBV) infection in military personnel in eastern China, which will provide a basis for the prevention of HBV infection.A total of 15,508 soldiers and 2386 officers were recruited from military camps in eastern China. The markers, deoxyribonucleic acid, serotypes, and genotypes of HBV in serum were detected and analyzed.Hepatitis B surface antigen (HBsAg) positive rate was 0.44% in soldiers, in whom the low-level HBsAg accounted for 88.24%. The HBsAg positive rate was 1.72% in officers in whom the low-level HBsAg accounted for 12.20%. There were significant differences in the prevalence of high-level and low-level HBsAg, HBV serotypes, HBV DNA positive rate, and mean log HBV DNA between officers and soldiers (P < .05). Compared with the conventional method for HBV DNA extraction, the enrichment method for HBV DNA extraction could significantly improve the positive rate and quantification of HBV DNA by real-time fluorescence quantitative polymerase chain reaction (P < .05). Sequencing of S gene in HBV was used for the determination of serotype and genotype of HBV. The sequencing success rate was significantly different between soldiers and officers (P < .05) as well as between high-level HBsAg group and low-level HBsAg group (P < .05). Significant difference was also observed in the genotype distribution between soldiers and officers (P < .05).HBV infection displays a low prevalence and a low epidemic state, and the prevalence of low-level HBsAg is higher in soldiers. We should pay attention to improve the quality of conscription examination as well as emphasize the surveillance, prevention, and protection of HBV infection in military officers.

PMID:
    30235666
DOI:
    10.1097/MD.0000000000012201

StephenW

医学（巴尔的摩）。 2018年9月; 97（38）：e12201。 doi：10.1097 / MD.0000000000012201。
中国东部军营HBV感染和低水平HBsAg的流行病学调查。
Wang T1,2，Dai Y2，Lu W2，Zhou H2，Chen Y3，Xu X4，Sun C2，Cheng J2。
作者信息

1
    蚌埠医学院研究生院。
2
    解放军第117医院临床实验室。
3
    浙江大学医学院附属第一医院传染病诊疗国家重点实验室，杭州
4
    日本东京大学生物技术系。

抽象

本研究旨在调查中国东部军人乙型肝炎病毒（HBV）感染情况，为预防乙型肝炎病毒感染提供依据。中国东部军营共招募了15,508名士兵和2386名军官。检测并分析血清中HBV的标志物，脱氧核糖核酸，血清型和基因型。士兵乙型肝炎表面抗原（HBsAg）阳性率为0.44％，其中低水平HBsAg占88.24％。 HBsAg阳性率为1.72％，低水平HBsAg占12.20％。高水平和低水平HBsAg，HBV血清型，HBV DNA阳性率和官兵之间HBV DNA平均值的患病率存在​​显着差异（P <.05）。与传统的HBV DNA提取方法相比，HBV DNA提取的富集方法可以通过实时荧光定量聚合酶链反应显着提高HBV DNA的阳性率和定量（P <.05）。 HBV中S基因的测序用于测定HBV的血清型和基因型。士兵和军官（P <.05）以及高水平HBsAg组和低水平HBsAg组之间的测序成功率差异显着（P <.05）。士兵和军官之间的基因型分布也存在显着差异（P <.05）.HBV感染率较低，流行状态较低，士兵中低水平HBsAg的患病率较高。要注意提高征兵检查质量，注重对军官HBV感染的监测，预防和保护。

结论：
    30235666
DOI：
    10.1097 / MD.0000000000012201