HBV相关性肝细胞癌肝脏的研究：体视学显示肝脏结构的定量

StephenW

Eur J Histochem. 2018 Sep 18;62(3). doi: 10.4081/ejh.2018.2950.
Study of liver in HBV-related hepatocellular carcinoma: Stereology shows quantitative differences in liver structure.
Moudi B1, Heidari Z, Mahmoudzadeh-Sagheb H.
Author information

1
    Zahedan University of Medical Sciences, Infectious Diseases and Tropical Medicine Research Center, and Department of Histology. [email protected].

Abstract

Hepatocellular carcinoma is one of the main consequences of liver chronic disease. Hepatocellular carcinoma-related changes may be seen in patients with chronic hepatitis B. The aim of the current study was to quantitate liver tissue elements by stereological technique in patients with hepatitis B-related cancer and compare the results with control and only hepatitis B group. Needle liver biopsies from 40 patients with only chronic hepatitis B infection, from 41 patients with only early hepatocellular carcinoma, from 40 patients with early hepatitis B-related cancer and 30 healthy subjects (control group) were analyzed by stereological method using systematic uniform random sampling method. Haematoxylin and eosin stained sections were used. The numerical density of hepatocytes, hepatocyte volume, numerical density of Kupffer cells, volume density of the connective tissue in the portal space, and volume density of the connective tissue were assessed. Quantitative analysis of liver samples indicated that there were statistically significant differences in the numerical density of hepatocytes, hepatocyte volume, numerical density of Kupffer cells, volume density of the connective tissue in the portal space, and volume density of the connective tissue between control and hepatitis B-related cancer and hepatitis B groups. Quantitative, stereological technique is simple and reliable for evaluating HCC in chronic hepatitis B. It is useful for assessing the liver tissue parameters. Stereology is recommended for the diagnosis of people prone to cancer in patients with chronic hepatitis B.

PMID:
    30223640
DOI:
    10.4081/ejh.2018.2950

StephenW

Eur J Histochem。 2018年9月18日; 62（3）。 doi：10.4081 / ejh.2018.2950。
HBV相关性肝细胞癌肝脏的研究：体视学显示肝脏结构的定量差异。
Moudi B1，Heidari Z，Mahmoudzadeh-Sagheb H.
作者信息

1
    扎黑丹医科大学，传染病与热带医学研究中心，组织学系。 [email protected]。

抽象

肝细胞癌是肝脏慢性疾病的主要后果之一。慢性乙型肝炎患者可能出现与肝细胞癌相关的变化。本研究的目的是通过体视学技术对乙型肝炎相关癌症患者的肝组织元素进行定量，并将结果与​​对照组和仅乙型肝炎组进行比较。 40例仅患有慢性乙型肝炎的患者，41例仅患有早期肝细胞癌的患者，40例早期乙型肝炎相关癌症患者和30例健康受试者（对照组）的针肝活组织检查采用系统均匀随机抽样的体视学方法进行分析方法。使用苏木精和伊红染色的切片。评估肝细胞的数密度，肝细胞体积，库普弗细胞的数密度，门静脉空间中结缔组织的体积密度和结缔组织的体积密度。肝脏样本的定量分析表明，肝细胞数量密度，肝细胞体积，库普弗细胞数量密度，门静脉间隙结缔组织体积密度，对照组与肝炎之间结缔组织的体积密度存在统计学差异。 B相关癌症和乙型肝炎组。定量，体视学技术简单可靠，可用于评估慢性乙型肝炎患者的HCC。它可用于评估肝脏组织参数。建议将体视学用于诊断慢性乙型肝炎患者易患癌症的人群。

结论：
    30223640
DOI：
    10.4081 / ejh.2018.2950

StephenW

https://www.ejh.it/index.php/ejh/article/view/2950