Lnc-DC通过树突状细胞中的TLR9 / STAT3信号传导调节细胞更新和HB

StephenW

Cell Mol Biol Lett. 2018 Sep 3;23:43. doi: 10.1186/s11658-018-0108-y. eCollection 2018.
Lnc-DC regulates cellular turnover and the HBV-induced immune response by TLR9/STAT3 signaling in dendritic cells.
Zhuang L1, Tian J1, Zhang X1, Wang H1, Huang C1,2.
Author information

1
    1Department of Infectious Disease, the Affiliated Shenzhen Baoan Hospital of Southern Medical University, Shenzhen, 518101 China.
2
    Department of Infectious Disease, Shenzhen Baoan District People's Hospital, No. 118, Xin'an Street, Long Jing er Raod, Shenzhen, 518101 China.

Abstract
Background:

Lnc-DC is a specific group of long non-coding (Lnc) RNAs in dendritic cells (DCs). Its function has been previously studied, and includes roles in dendritic cell differentiation and the progression of some diseases. In this study, we observed the critical role of Lnc-DC in regulating the differentiation, growth, and apoptosis of dendritic cells.
Methods:

We first isolated peripheral blood mononuclear cells to culture and induce into DCs, which were then co-cultured with hepatitis B virus (HBV)-secreting HepG2.2.15 cells for the detection of changes in Lnc-DC. The expression levels of TLR9, p-STAT3, and SOCS3 were tested with qPCR and western blot. MTT assays were used to analyze the cell proliferation, cell cycle, and apoptosis. We used ELISA to test the expression of TNF-α, IL-1β, IL-6, IL-12p40, and IFN-γ.
Results:

Co-culture with HBV-secreting HepG2.2.15 cells increased the level of Lnc-DC and activated TLR9/STAT3 signaling. The HBV DNA level (IU/ml) was positively correlated with levels of Lnc-DC and TLR9, further demonstrating that Lnc-DC was associated with the immune response of HBV. Lnc-DC was shown to regulate TLR9/STAT3 signaling in dendritic cells. More interestingly, the regulation of Lnc-DC controlled the immune response by reducing the concentration of secreted TNF-α, IL-6, IL-12, and IFN-γ, as well as increasing the IL-1β concentration in dendritic cells.
Conclusion:

Lnc-DC is important in regulating the growth, apoptosis, and immune response of dendritic cells mediated by TLR9/STAT3 signaling, and was also activated by HBV. This study provides a previously unidentified mechanism underlying the immune response in dendritic cells.
KEYWORDS:

Dendritic cell; HBV; Lnc-DC; TLR9/STAT3

PMID:
    30202418
PMCID:
    PMC6122708
DOI:
    10.1186/s11658-018-0108-y

StephenW

Cell Mol Biol Lett。 2018年9月3日; 23:43。 doi：10.1186 / s11658-018-0108-y。 eCollection 2018。
Lnc-DC通过树突状细胞中的TLR9 / STAT3信号传导调节细胞更新和HBV诱导的免疫应答。
庄L1，田J1，张X1，王H1，黄C1,2。
作者信息

1
    1南方医科大学附属深圳市宝安医院传染病科，深圳518101
2
    深圳市宝安区人民医院传染病科，深圳市龙井镇新安街118号，邮编：518101。

抽象
背景：

Lnc-DC是树突细胞（DC）中特定的长非编码（Lnc）RNA组。其功能先前已被研究，并且包括在树突细胞分化和一些疾病的进展中的作用。在本研究中，我们观察了Lnc-DC在调节树突细胞分化，生长和凋亡中的关键作用。
方法：

我们首先将外周血单个核细胞分离培养并诱导成DC，然后与乙型肝炎病毒（HBV）共培养，分泌HepG2.2.15细胞，检测Lnc-DC的变化。用qPCR和蛋白质印迹测试TLR9，p-STAT3和SOCS3的表达水平。 MTT测定用于分析细胞增殖，细胞周期和细胞凋亡。我们使用ELISA来检测TNF-α，IL-1β，IL-6，IL-12p40和IFN-γ的表达。
结果：

与分泌HBV的HepG2.2.15细胞共培养增加了Lnc-DC和激活的TLR9 / STAT3信号传导的水平。 HBV DNA水平（IU / ml）与Lnc-DC和TLR9水平呈正相关，进一步证明Lnc-DC与HBV的免疫应答有关。显示Lnc-DC调节树突细胞中的TLR9 / STAT3信号传导。更有趣的是，Lnc-DC的调节通过降低分泌的TNF-α，IL-6，IL-12和IFN-γ的浓度以及增加树突细胞中的IL-1β浓度来控制免疫应答。
结论：

Lnc-DC在调节由TLR9 / STAT3信号传导介导的树突细胞的生长，凋亡和免疫应答中是重要的，并且也被HBV激活。该研究提供了一种先前未确定的机制，其基础是树突细胞中的免疫应答。
关键词：

树突细胞; HBV; LNC-DC; TLR9 / STAT3

结论：
    30202418
PMCID：
    PMC6122708
DOI：
    10.1186 / s11658-018-0108-Y