低锌指抗病毒蛋白表达与肝细胞癌患者进展和生存能力差的

StephenW

Cell Physiol Biochem. 2018 Sep 7;49(3):1007-1018. doi: 10.1159/000493285. [Epub ahead of print]
Association of Low Zinc Finger Antiviral Protein Expression with Progression and Poor Survival of Patients with Hepatocellular Carcinoma.
Liu Y1,2, Hu C3, Zhu WB4, Xu WX1,2, Li ZY1,2, Lin Y4, Cai J4, Liang JK4, Zhu X1,2, Gao ZL1,2, Yan GM4,5, Peng L1,2.
Author information

1
    Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
2
    Guangdong Key Laboratory of Liver Disease Research, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
3
    Department of Urology Diseases, Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
4
    Departments of Pharmacology, Sun Yat-sen University, Guangzhou, China.
5
    Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.

Abstract
BACKGROUND/AIMS:

Zinc finger antiviral protein (ZAP) has been reported to be expressed in hepatocellular carcinoma (HCC), and ZAP expression is associated with apoptotic signaling in cancer cells. This study aimed at investigating the expression of ZAP in HCC cells and its significance in clinical pathology.
METHODS:

Real-time quantitative PCR and western blot assays were employed to detect ZAP RNA and protein expression in normal human hepatocytes, HCC cells, and five primary HCC cell lines. Immunohistochemistry was performed to detect ZAP expression in 147 paraffin-embedded HCC tissues and adjacent normal tissues. The clinical significance of ZAP expression was analyzed in tissue samples from patients with or without infection by hepatitis B virus (HBV).
RESULTS:

ZAP expression in HCC cells and human primary HCC cell lines was significantly lower than that of normal human hepatocytes. Among 147 HCC samples, ZAP expression was lower in HCC tissues than in adjacent normal tissues for 107 (77.0%) samples. In patients with HCC and HBV infection, ZAP expression was related to pathological grade (P < 0.05); in HBV-negative patients with HCC, ZAP expression was associated with tumor size (P < 0.05) and clinical stage (P < 0.05). The overall survival time in patients with low ZAP expression was significantly shorter than survival times of those with high ZAP expression (P < 0.05), especially for patients with moderately to well-differentiated HCC (Grade 1-2) and HCC at stage T1 and T2 (P < 0.05). Cox multivariate analysis showed that ZAP expression was an independent predictor of survival of patients with HCC (P < 0.01).
CONCLUSION:

Low ZAP expression is closely associated with disease progression and poor prognosis for patients with HCC.

© 2018 The Author(s). Published by S. Karger AG, Basel.
KEYWORDS:

Biomarker; Hepatocellular carcinoma; Prognosis; ZAP; Zinc antiviral protein

PMID:
    30196292
DOI:
    10.1159/000493285

StephenW

Cell Physiol Biochem。 2018年9月7日; 49（3）：1007-1018。 doi：10.1159 / 000493285。 [提前打印]
低锌指抗病毒蛋白表达与肝细胞癌患者进展和生存能力差的关系。
Liu Y1,2，Hu C3，Zhu WB4，Xu WX1,2，Li ZY1,2，Lin Y4，Cai J4，Liang JK4，Zhu X1,2，Gao ZL1,2，Yan GM4,5，Peng L1,2。
作者信息

1
    中山大学附属第三医院传染病科，广州
2
    中山大学附属第三医院广东省肝病研究重点实验室，广州
3
    中山大学附属第三医院泌尿外科，广州
4
    中山大学药理学系，广州，中国。
五
    中国广州癌症医学协同创新中心。

抽象
背景/目的：

据报道，锌指抗病毒蛋白（ZAP）在肝细胞癌（HCC）中表达，并且ZAP表达与癌细胞中的凋亡信号传导相关。本研究旨在探讨ZAP在HCC细胞中的表达及其在临床病理中的意义。
方法：

使用实时定量PCR和蛋白质印迹分析来检测正常人肝细胞，HCC细胞和五种原代HCC细胞系中的ZAP RNA和蛋白质表达。进行免疫组织化学以检测147个石蜡包埋的HCC组织和邻近的正常组织中的ZAP表达。在患有或不患有乙型肝炎病毒（HBV）的患者的组织样品中分析ZAP表达的临床意义。
结果：

HCC细胞和人原代HCC细胞系中的ZAP表达显着低于正常人肝细胞。在147个HCC样本中，对于107个（77.0％）样本，在HCC组织中ZAP表达低于在邻近正常组织中的ZAP表达。在HCC和HBV感染患者中，ZAP表达与病理分级有关（P <0.05）;在HBV阴性的HCC患者中，ZAP表达与肿瘤大小（P <0.05）和临床分期（P <0.05）有关。 ZAP低表达患者的总生存时间明显短于ZAP高表达患者的生存时间（P <0.05），特别是对于中度至高分化HCC（1-2级）和T1期HCC患者。 T2（P <0.05）。 Cox多变量分析显示，ZAP表达是HCC患者生存的独立预测因子（P <0.01）。
结论：

低ZAP表达与HCC患者的疾病进展和预后不良密切相关。

©2018作者。由巴塞尔的S. Karger AG出版。
关键词：

生物标志物;肝细胞癌;预后; ZAP;锌抗病毒蛋白

结论：
    30196292
DOI：
    10.1159 / 000493285

StephenW

https://www.karger.com/Article/FullText/493285