小核仁RNA宿主基因18在肝细胞癌中作为肿瘤抑制因子和诊断指

StephenW

Technol Cancer Res Treat. 2018 Jan 1;17:1533033818794494. doi: 10.1177/1533033818794494.
Small Nucleolar RNA Host Gene 18 Acts as a Tumor Suppressor and a Diagnostic Indicator in Hepatocellular Carcinoma.
Liu XF1, Thin KZ1, Ming XL1, Shuo-Li1, Ping-Luo1, Man-Zhu1, Li ND1, Tu JC1.
Author information

1
    1 Department of Clinical Laboratory Medicine and Center for Gene Diagnosis, Zhongnan Hospital of Wuhan University, Wuhan, China.

Abstract
BACKGROUND:

Noncoding RNAs are crucial regulators acting as either tumor suppressor genes or oncogenes in human cancer progression. The aberrant expression of noncoding RNAs has been confirmed in different kinds of cancers. Hepatocellular carcinoma is one of the most common malignant tumors worldwide, characterized by insidious onset, great malignancy, and high rates of recurrence and metastasis. Due to lack of early predictive markers, numerous patients are diagnosed in the late stages. As therapeutic options for advanced patients are quite limited, great efforts have been made to screen patients at early stages. A previous study reported that small nucleolar RNA host gene 18 played crucial role in glioma. However, its functions and roles in hepatocellular carcinoma are unknown.
PURPOSE:

To explore its functional role and diagnostic value in hepatocellular carcinoma, we investigated its expression level.
METHODS:

We performed real-time quantitative polymerase chain reaction in tumor tissues and adjacent noncancerous tissues derived from patients with hepatocellular carcinoma as well as in plasma, including samples from the healthy control, patients with hepatitis B, cirrhosis, and hepatocellular carcinoma.
RESULTS:

Small nucleolar RNA host gene 18 was downregulated in liver tissues compared to paired adjacent noncancerous tissues ( P < .0001). Meanwhile, plasma small nucleolar RNA host gene 18 showed a relatively high sensitivity and specificity (75.61% and 73.49%) for distinguishing patients with hepatocellular carcinoma whose α-fetoprotein levels were below 200 ng/mL from the healthy controls.
CONCLUSION:

Our study suggested that small nucleolar RNA host gene 18 might act as a tumor suppressor gene in hepatocellular carcinoma and potentially a diagnostic indicator to distinguish hepatocellular carcinoma from the healthy control and cirrhosis.
KEYWORDS:

HCC; SNHG18; diagnostic marker; noncoding RNA; tumor suppressor gene

PMID:
    30126319
DOI:
    10.1177/1533033818794494

StephenW

Technol癌症治疗。 2018年1月1日; 17：1533033818794494。 doi：10.1177 / 1533033818794494。
小核仁RNA宿主基因18在肝细胞癌中作为肿瘤抑制因子和诊断指标。
刘XF1，薄KZ1，明XL1，硕丽1，平罗1，满朱1，李ND1，涂JC1。
作者信息

1
    1武汉大学中南医院临床检验医学与基因诊断中心，武汉

抽象
背景：

非编码RNA是在人类癌症进展中充当肿瘤抑制基因或癌基因的关键调节剂。非编码RNA的异常表达已在不同类型的癌症中得到证实。肝细胞癌是世界上最常见的恶性肿瘤之一，其特征是起病隐匿，恶性程度高，复发和转移率高。由于缺乏早期预测标志物，许多患者在晚期被诊断出来。由于对于晚期患者的治疗选择非常有限，因此已经做出巨大努力来在早期阶段筛查患者。先前的一项研究报道，小核仁RNA宿主基因18在胶质瘤中起着至关重要的作用。然而，其在肝细胞癌中的功能和作用尚不清楚。
目的：

为了探讨其在肝细胞癌中的功能作用和诊断价值，我们研究了其表达水平。
方法：

我们在来自肝细胞癌患者和血浆的肿瘤组织和邻近的非癌组织中进行了实时定量聚合酶链反应，包括来自健康对照，乙型肝炎患者，肝硬化和肝细胞癌的样品。
结果：

与成对的相邻非癌组织相比，小核仁RNA宿主基因18在肝组织中下调（P <.0001）。同时，血浆小核仁RNA宿主基因18显示出较高的敏感性和特异性（75.61％和73.49％），用于区分健康对照组中甲胎蛋白水平低于200 ng / mL的肝细胞癌患者。
结论：

我们的研究表明，小核仁RNA宿主基因18可能在肝细胞癌中起到肿瘤抑制基因的作用，并可能成为区分肝细胞癌与健康对照和肝硬化的诊断指标。
关键词：

HCC; SNHG18;诊断标记;非编码RNA;肿瘤抑制基因

结论：
    30126319
DOI：
    10.1177 / 1533033818794494