慢性乙型肝炎和肝细胞癌患者乙型肝炎核心相关抗原与HBV病

StephenW

Clin Chim Acta. 2018 Jul 16. pii: S0009-8981(18)30374-7. doi: 10.1016/j.cca.2018.07.027. [Epub ahead of print]
Correlation between hepatitis B core-related antigen and HBV viral load in chronic hepatitis B and hepatocellular carcinoma patients.
Chen S1, Jia J2, Gao Y2, Li H2, Fang M2, Feng H2, Guan W1, Ji J2, Gao Z2, Gao C3.
Author information

1
    Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China; The First Clinical Medical College of Fujian Medical University, Fuzhou, PR China.
2
    Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China.
3
    Department of Laboratory Medicine, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, PR China. Electronic address: [email protected].

Abstract
BACKGROUND:

Hepatitis B core-related antigen has been revealed as an important marker of HBV infection recently. We aimed to evaluate the HBcrAg assay for indication of HBV loads in chronic hepatitis B (CHB) and hepatocellular carcinoma patients (HCC).
METHODS:

HBcrAg was measured by chemiluminescence enzyme immunoassay. Intrahepatic covalently closed circular DNA (cccDNA) was measured by real-time PCR with TaqMan fluorescent probes based on liver biopsy specimens from HCC patients.
RESULTS:

HBcrAg correlated positively with HBV DNA irrespective of HBeAg status. Both HBcrAg and HBV DNA were associated with cccDNA in patients with elevated serum HBV DNA (>4 log IU/ml). In patients with non-elevated HBV DNA (≤4 log IU/ml), no relationship between HBV DNA and cccDNA was observed, but we still documented a modest correlation between HBcrAg and cccDNA. In addition, a decreased correlation between HBcrAg and cccDNA in HCC tumorigenesis was documented. Finally, a moderate correlation occurred between HBcrAg and albumin, alanine transaminase, aspartate transaminase in both CHB and HCC patients.
CONCLUSIONS:

Our data show that HBcrAg can serve as a biomarker to assess HBV loads in patients and provide a better method for monitoring cccDNA in HCC patients than HBV DNA.

Copyright © 2018. Published by Elsevier B.V.
KEYWORDS:

Chronic hepatitis B (CHB); Covalently closed circular DNA (cccDNA); HBV DNA; HBcrAg; Hepatocellular carcinoma (HCC)

PMID:
    30025756
DOI:
    10.1016/j.cca.2018.07.027

StephenW

Clin Chim Acta。 2018年7月16日.pii：S0009-8981（18）30374-7。 doi：10.1016 / j.cca.2018.07.027。 [提前打印]
慢性乙型肝炎和肝细胞癌患者乙型肝炎核心相关抗原与HBV病毒载量的相关性。
Chen S1，Jia J2，Gao Y2，Li H2，Fang M2，Feng H2，Guan W1，Ji J2，Gao Z2，Gao C3。
作者信息

1
    第二军医大学东方肝胆外科医院检验科，上海;福建医科大学第一临床医学院，福州，中国。
2
    第二军医大学东方肝胆外科医院检验科，上海。
3
    第二军医大学东方肝胆外科医院检验科，上海。电子地址：[email protected]。

抽象
背景：

乙型肝炎核心相关抗原最近被揭示为HBV感染的重要标志物。我们的目的是评估HBcrAg检测对慢性乙型肝炎（CHB）和肝细胞癌患者（HCC）HBV负荷的指征。
方法：

通过化学发光酶免疫测定法测量HBcrAg。通过基于来自HCC患者的肝活组织检查标本的TaqMan荧光探针的实时PCR测量肝内共价闭合环状DNA（cccDNA）。
结果：

无论HBeAg状态如何，HBcrAg与HBV DNA呈正相关。 HBcrAg和HBV DNA均与血清HBV DNA升高（> 4 log IU / ml）患者的cccDNA相关。在HBV DNA未升高（≤4logIU/ ml）的患者中，没有观察到HBV DNA和cccDNA之间的关系，但我们仍然记录了HBcrAg和cccDNA之间的适度相关性。此外，记录了HCC肿瘤发生中HBcrAg和cccDNA之间的相关性降低。最后，CHB和HCC患者中HBcrAg与白蛋白，丙氨酸转氨酶，天冬氨酸转氨酶之间存在中度相关性。
结论：

我们的数据显示，HBcrAg可作为评估患者HBV负荷的生物标志物，并为监测HCC患者的cccDNA提供了比HBV DNA更好的方法。

版权所有©2018。Elsevier B.V.
关键词：

慢性乙型肝炎（CHB）;共价闭合环状DNA（cccDNA）; HBV DNA; HBcrAg;肝细胞癌（HCC）

结论：
    30025756
DOI：
    10.1016 / j.cca.2018.07.027