血清HBV DNA检测在HBV感染患者中的应用策略：5611例标本的回顾

StephenW

    Format: Abstract

Send to
J Med Virol. 2018 Jul 16. doi: 10.1002/jmv.25271. [Epub ahead of print]
Application strategies of serum HBV DNA detection in HBV infection patients: a retrospective study of 5611 specimens.
Liu C1,2, Wu W1,2, Shang S1,2, Huang E1,2, Xun Z1,2, Lin J1,2, Chen T1,2, Yang B1,2, Chen J3, Ou Q1,2.
Author information

1
    Department of Laboratory Medicine, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, China.
2
    Gene Diagnostic Laboratory, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian Province, China.
3
    Liver Research Center, The First Affiliated Hospital of Fujian Medical University.

Abstract

The detection of HBV DNA plays a critical role in determining the level of viral replication in HBV infected patients. However, how to select appropriate HBV DNA detection method, low-sensitivity (ls) and hypersensitivity (hs), remains unclear. In this study, HBsAg, HBeAg, ALT, AST and hs HBV DNA titers in serum of 5611 cases with suspected HBV infection were reviewed. Besides, the dynamic changes of HBV DNA and HBsAg in 85 chronic hepatitis B (CHB) patients receiving PegIFNα or entecavir (ETV) were observed. The results showed the positive rate of HBV DNA was 32.8%, of which low viral load (20~500 IU/ml) accounted for 51.8%. In the 5611 cases, when the HBsAg<1000 IU/ml, the proportion of low viral load was 76.3%. Moreover, in patients receiving antiviral treatment, when HBsAg<2000 IU/ml (PegIFNα) or HBsAg<3500 IU/ml (ETV), the proportion of patients with low viral load was 79.5% or 78.0%, respectively. We developed a strategy of serum HBV DNA detection in HBV infection patients. When HBsAg was negative, HBV DNA detection should be unnecessary. When HBsAg was 0.05~1000 IU/ml, hs HBV DNA should be detected in patients with abnormal level of ALT, AST or HBeAg. While HBsAg was ≥1000 IU/ml, ls HBV DNA was recommended. Moreover, the cut-off value of HBsAg increased during antiviral therapy of CHB patients. In conclusion, hs HBV DNA is of great value in HBV infected patients with low viral load. HBV DNA detection methods should be selected reasonably according to the levels of HBsAg, HBeAg, ALT and AST. This article is protected by copyright. All rights reserved.
KEYWORDS:

HBV DNA; clinical detection pathway; hepatitis B virus; quantitative HBsAg

PMID:
    30011350
DOI:
    10.1002/jmv.25271

StephenW

J Med Virol。 2018年7月16日doi：10.1002 / jmv.25271。 [提前打印]
血清HBV DNA检测在HBV感染患者中的应用策略：5611例标本的回顾性研究。
Liu C1,2，Wu W1,2，Shang S1,2，Huang E1,2，Xun Z1,2，Lin J1,2，Chen T1,2，Yang B1,2，Chen J3，Ou Q1,2。
作者信息

1
    福建医科大学第一附属医院检验科，福建省福州市
2
    福建医科大学第一附属医院基因诊断实验室，福建省福州市
3
    福建医科大学附属第一医院肝脏研究中心。

抽象

HBV DNA的检测在确定HBV感染患者的病毒复制水平中起关键作用。然而，如何选择合适的HBV DNA检测方法，低敏感性（ls）和超敏反应（hs）仍不清楚。本研究回顾了5611例疑似HBV感染患者血清中HBsAg，HBeAg，ALT，AST和hs HBV DNA滴度的变化。此外，观察了接受PegIFNα或恩替卡韦（ETV）的85例慢性乙型肝炎（CHB）患者HBV DNA和HBsAg的动态变化。结果显示HBV DNA阳性率为32.8％，其中低病毒载量（20~500 IU / ml）占51.8％。在5611例中，当HBsAg <1000 IU / ml时，低病毒载量的比例为76.3％。此外，在接受抗病毒治疗的患者中，当HBsAg <2000 IU / ml（PegIFNα）或HBsAg <3500 IU / ml（ETV）时，低病毒载量的患者比例分别为79.5％或78.0％。我们制定了HBV感染患者血清HBV DNA检测策略。当HBsAg阴性时，HBV DNA检测应该是不必要的。当HBsAg为0.05~1000 IU / ml时，应检测到ALT，AST或HBeAg水平异常的患者的HBs DNA。虽然HBsAg≥1000IU/ ml，但推荐使用HBs DNA。此外，在CHB患者的抗病毒治疗期间，HBsAg的临界值增加。总之，hs HBV DNA在低病毒载量的HBV感染患者中具有重要价值。应根据HBsAg，HBeAg，ALT和AST的水平合理选择HBV DNA检测方法。本文受版权保护。版权所有。
关键词：

HBV DNA;临床检测途径;乙型肝炎病毒;定量HBsAg

结论：
    30011350
DOI：
    10.1002 / jmv.25271