怀孕和未怀孕的育龄妇女的乙型肝炎病毒载量和病毒抗原水

StephenW

BMC Pregnancy Childbirth. 2018 Jul 6;18(1):292. doi: 10.1186/s12884-018-1932-9.
Comparison of hepatitis B viral loads and viral antigen levels in child-bearing age women with and without pregnancy.
Xu C1, Liu J2, Liu L3, Bi Y2, Xu B4, Chen J5, Xu B3, Chen T1, Hu Y5, Zhou YH6,7.
Author information

1
    Department of Obstetrics and Gynecology, Zhenjiang Fourth People's Hospital, Zhenjiang, 212001, Jiangsu, China.
2
    Departments of Laboratory Medicine and Infectious Diseases, Nanjing Drum Tower Hospital and Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, 321 Zhongshan Road, Nanjing, 210008, Jiangsu, China.
3
    Department of Obstetrics and Gynecology, Taixing People's Hospital, Taixing, 225400, Jiangsu, China.
4
    Department of Biostatistics, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, 210008, Jiangsu, China.
5
    Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, 210008, Jiangsu, China.
6
    Departments of Laboratory Medicine and Infectious Diseases, Nanjing Drum Tower Hospital and Jiangsu Key Laboratory for Molecular Medicine, Nanjing University Medical School, 321 Zhongshan Road, Nanjing, 210008, Jiangsu, China. [email protected].
7
    Department of Infectious Diseases, Nanjing Drum Tower Hospital, Nanjing Medical University, Nanjing, 210008, Jiangsu, China. [email protected].

Abstract
BACKGROUND:

Pregnancy is a unique physiological condition with the cellular immune functions compromised at some extents to allow the mature of growing fetus. Whether pregnancy may influence the replication of hepatitis B virus (HBV) is less studied. The present study aimed to investigate the influence of pregnancy on the replication of HBV and expression of viral antigens by comparing the levels of HBV DNA and viral antigens in pregnant and non-pregnant women.
METHODS:

A total of 727 HBsAg-positive serum samples, collected from 214 pregnant women and 513 non-pregnant women of childbearing age, were included. Based on the pregnancy status, subjects were divided into four groups: nulliparous (n = 158), pregnant (n = 214), 7-12 months postpartum (n = 170), and 2-5 years postpartum (n = 185). The levels of hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) were quantitatively measured with microparticle enzyme immunoassay. HBV DNA levels were detected by fluorescent real-time PCR.
RESULTS:

The median ages of four groups were 25.0, 25.3, 26.2 and 29.3 years, respectively (p < 0.01). HBeAg-positive proportions were 34.2, 33.6, 35.3 and 29.2%, respectively (p = 0.624). HBV DNA levels in HBeAg-positive women were higher than those in HBeAg-negative women (7.88 vs 2.62 log IU/ml, p < 0.001). HBV DNA levels in the four groups with positive HBeAg were 7.8, 7.7, 8.0 and 8.0 log IU/ml, respectively (p = 0.057), while HBsAg titers were 4.4, 4.5, 4.6 and 4.8 log IU/ml (p = 0.086) and HBeAg titers were 3.1, 3.0, 3.1 and 3.0 log S/CO (p = 0.198). In the four groups with negative HBeAg, HBV DNA levels were 2.3, 2.6, 2.5 and 2.8 log IU/ml, respectively (p = 0.085), while HBsAg titers were 3.1, 3.3, 3.3 and 3.0 log IU/ml (p = 0.06).
CONCLUSIONS:

Serum levels of HBV DNA and viral antigens showed no significant changes in nulliparous, pregnant, and postpartum women, regardless of the HBeAg status. The results indicate that pregnancy has little influence on the replication of HBV and the expression of viral antigens.
KEYWORDS:

Expression of HBsAg and HBeAg; HBV replication; Pregnancy

PMID:
    29980185
DOI:
    10.1186/s12884-018-1932-9

StephenW

BMC怀孕分娩。 2018年7月6日; 18（1）：292。 doi：10.1186 / s12884-018-1932-9。
怀孕和未怀孕的育龄妇女的乙型肝炎病毒载量和病毒抗原水平的比较。
Xu C1，Liu J2，Liu L3，Bi Y2，Xu B4，Chen J5，Xu B3，Chen T1，Hu Y5，Zhou YH6,7。
作者信息

1
    镇江市第四人民医院妇产科，江苏镇江212001
2
    中国南京市中山路321号南京大学医学院南京鼓楼医院检验医学与传染病科，江苏省分子医学重点实验室，江苏南京210008
3
    泰兴市人民医院妇产科，江苏泰兴225400
4
    南京大学医学院南京鼓楼医院生物统计学系，江苏南京210008
五
    南京大学医学院南京鼓楼医院妇产科，江苏南京210008
6
    中国南京市中山路321号南京大学医学院南京鼓楼医院检验医学与传染病科，江苏省分子医学重点实验室，江苏南京210008 [email protected]。
7
    南京医科大学南京鼓楼医院感染科，江苏南京210008 [email protected]。

抽象
背景：

妊娠是一种独特的生理状态，细胞免疫功能在某种程度上受到损害，使胎儿成熟。怀孕是否可能影响乙型肝炎病毒（HBV）的复制研究较少。本研究旨在通过比较孕妇和非孕妇的HBV DNA和病毒抗原水平，探讨妊娠对HBV复制和病毒抗原表达的影响。
方法：

共收集了来自214名孕妇和513名育龄非孕妇的727份HBsAg阳性血清样本。根据妊娠状况，将受试者分为四组：未经产（n = 158），怀孕（n = 214），产后7-12个月（n = 170）和产后2 - 5年（n = 185）。用微粒酶免疫测定法定量测定乙型肝炎表面抗原（HBsAg）和乙型肝炎e抗原（HBeAg）的水平。通过荧光实时PCR检测HBV DNA水平。
结果：

四组的中位年龄分别为25.0,25.3,26.2和29.3岁（p <0.01）。 HBeAg阳性比例分别为34.2,33.6,35.3和29.2％（p = 0.624）。 HBeAg阳性女性的HBV DNA水平高于HBeAg阴性女性（7.88 vs 2.62 log IU / ml，p <0.001）。 HBeAg阳性的四组HBV DNA水平分别为7.8,7.7,8.0和8.0 log IU / ml（p = 0.057），而HBsAg滴度分别为4.4,4.5,4.6和4.8 log IU / ml（p = 0.086）和HBeAg滴度分别为3.1,3.0,3.1和3.0 log S / CO（p = 0.198）。在HBeAg阴性的四组中，HBV DNA水平分别为2.3,2.6,2.5和2.8 log IU / ml（p = 0.085），而HBsAg滴度分别为3.1,3.3,3.3和3.0 log IU / ml（p = 0.06） ）。
结论：

无论HBeAg状态如何，HBV DNA和病毒抗原的血清水平均未显示未生育，孕妇和产后妇女的显着变化。结果表明，妊娠对HBV的复制和病毒抗原的表达几乎没有影响。
关键词：

HBsAg和HBeAg的表达; HBV复制;怀孕

结论：
    29980185
DOI：
    10.1186 / s12884-018-1932-9

Hepbest

这个结论的建立在平均年龄不超过30岁的孕妇身上，他们大多数处在免疫耐受期。
处在不同时期的孕妇差别可能很大，这一点很重要。

结论：
无论HBeAg状态如何，HBV DNA和病毒抗原的血清水平均未显示未生育，孕妇和产后妇女的显着变化。结果表明，妊娠对HBV的复制和病毒抗原的表达几乎没有影响。