干扰素刺激的基因20 kDa蛋白血清水平和乙型肝炎病毒相关肝

StephenW

Oncotarget. 2018 Jun 12;9(45):27858-27871. doi: 10.18632/oncotarget.25559. eCollection 2018 Jun 12.
Interferon-stimulated gene 20 kDa protein serum levels and clinical outcome of hepatitis B virus-related liver diseases.
Van Tong H1,2,3, Hoan NX3,4,5, Binh MT3,4,5, Quyen DT3,4,5, Meyer CG3,5,6, Song LH4,5, Toan NL2, Velavan TP3,5,6.
Author information

1
    Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam.
2
    Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Vietnam.
3
    Institute of Tropical Medicine, University of Tübingen, Tübingen, Germany.
4
    108 Military Central Hospital, Hanoi, Vietnam.
5
    Vietnamese-German Center of Excellence in Medical Research, Hanoi, Vietnam.
6
    Duy Tan University, Da Nang, Vietnam.

Abstract

Interferon-stimulated gene 20 kDa protein (ISG20) with 3' to 5' exonuclease activity mainly targeting single-stranded RNA plays an important role in immune responses against various infectious pathogens, including hepatitis viruses. ISG20 levels were measured by ELISA assays in sera of 339 hepatitis B-virus (HBV) infected patients and 71 healthy individuals and were correlated with clinical and laboratory parameters. ISG20 mRNA was quantified by qRT-PCR in 30 pairs of hepatocellular carcinoma (HCC) tumour and adjacent non-tumour liver tissues. ISG20 levels were significantly elevated in HBV patients compared to healthy controls (P<0.0001). In the patient group, varying ISG20 levels were associated with different forms of HBV-related liver diseases. ISG20 levels were higher in patients with HCC compared to those without HCC (P<0.0001), and increased according to the stages of HCC (P<0.0001). ISG20 mRNA expression was up-regulated in tumour tissues compared to the expression in adjacent non-tumour tissues (P=0.017). Importantly, ISG20 levels were strongly correlated with the levels of AST, ALT, total and direct bilirubin among HCC patients (Pearson's r = 0.43, 0.35, 0.34, 0.3; P<0.0001, respectively). Although differences between liver cirrhosis (LC) and non-LC patients were not observed, ISG20 levels were elevated according to the progression of cirrhosis in patients with LC plus HCC (P=0.005). In conclusions, ISG20 levels are induced by HBV infection and significantly associated with progression and clinical outcome of HBV-related liver diseases, especially in patients with HCC. ISG20 might be a potential indicator for liver injury and the clinical outcome in HBV-related HCC.
KEYWORDS:

ISG20 expression; ISG20 levels; hepatocellular carcinoma; interferon-stimulated gene; viral hepatitis

PMID:
    29963243
PMCID:
    PMC6021248
DOI:
    10.18632/oncotarget.25559

StephenW

Oncotarget。 2018年6月12日; 9（45）：27858-27871。 doi：10.18632 / oncotarget.25559。 eCollection 2018年6月12日。
干扰素刺激的基因20 kDa蛋白血清水平和乙型肝炎病毒相关肝病的临床结果。
Van Tong H1,2,3，Hoan NX3,4,5，Binh MT3,4,5，Quyen DT3,4,5，Meyer CG3,5,6，Song LH4,5，Toan NL2，Velavan TP3,5,6 。
作者信息

1
    越南军事医科大学生物医学与药学研究所，越南河内。
2
    越南军事医科大学病理生理学系，越南河内。
3
    德国蒂宾根蒂宾根大学热带医学研究所。
4
    108军事中心医院，越南河内。
五
    越南 - 德国医学研究卓越中心，越南河内。
6
    Duy Tan大学，越南岘港。

抽象

具有3'至5'外切核酸酶活性的干扰素刺激的基因20kDa蛋白（ISG20）主要靶向单链RNA，在针对各种感染性病原体（包括肝炎病毒）的免疫应答中起重要作用。通过ELISA测定在339名乙型肝炎病毒（HBV）感染患者和71名健康个体的血清中测量ISG20水平，并与临床和实验室参数相关联。通过qRT-PCR在30对肝细胞癌（HCC）肿瘤和相邻的非肿瘤肝组织中定量ISG20 mRNA。与健康对照相比，HBV患者的ISG20水平显着升高（P <0.0001）。在患者组中，不同的ISG20水平与不同形式的HBV相关的肝病相关。与没有HCC的患者相比，HCC患者的ISG20水平更高（P <0.0001），并且根据HCC的阶段增加（P <0.0001）。与邻近的非肿瘤组织中的表达相比，ISG20 mRNA表达在肿瘤组织中上调（P = 0.017）。重要的是，ISG20水平与HCC患者中AST，ALT，总胆红素和直接胆红素水平密切相关（Pearson's r = 0.43,0.35,0.34,0.3; P <0.0001）。虽然没有观察到肝硬化（LC）和非LC患者之间的差异，但根据LC加HCC患者的肝硬化进展，ISG20水平升高（P = 0.005）。总之，ISG20水平由HBV感染诱导，并且与HBV相关性肝病的进展和临床结果显着相关，尤其是在HCC患者中。 ISG20可能是肝损伤的潜在指标和HBV相关HCC的临床结果。
关键词：

ISG20表达; ISG20水平;肝细胞癌;干扰素刺激的基因;病毒性肝炎

结论：
    29963243
PMCID：
    PMC6021248
DOI：
    10.18632 / oncotarget.25559