慢性乙型肝炎病毒感染的肝纤维化临床预测因

StephenW

Clinical Predictors of Liver Fibrosis in Patients With Chronic Hepatitis B Virus Infection From Children to Adults
Jia-Feng Wu Shih-Hsi Song Chee-Seng Lee Huey-Ling Chen Yen-Hsuan Ni Hong-Yuan Hsu Tzee-Chung Wu Mei-Hwei Chang
The Journal of Infectious Diseases, Volume 217, Issue 9, 11 April 2018, Pages 1408–1416, https://doi.org/10.1093/infdis/jiy048
Published:
30 January 2018

Abstract
Background

This study aimed to elucidate predictors of liver fibrosis in patients with chronic hepatitis B virus (HBV) infection.
Methods

Transient elastography was performed to define liver stiffness in 533 patients with chronic HBV infection (mean age ± standard deviation, 30.72 ± 0.57 years). Protein array was performed on serum samples and lysates of Huh7 cells transfected with HBV mutants; the results were confirmed by enzyme-linked immunosorbent assay. Single-nucleotide polymorphisms in the gene encoding interleukin 1β (IL-1β) were examined in patients with chronic HBV infection with and without liver fibrosis.
Results

Male sex, age ≥18 years, and serum α-fetoprotein level >3.6 ng/mL were independent predictors of a liver stiffness measurement of ≥7 kPa (P = .005, .019, and <.001, respectively). HBV e antigen (HBeAg)–negative hepatitis is associated with increased liver stiffness (P < .001). Elevation of the serum IL-1β level was demonstrated in subjects with liver fibrosis. IL-1β was upregulated in Huh7 cells transfected with HBV mutants associated with HBeAg-negative hepatitis. The AA genotype at rs16944 and the CC genotype at rs1143627 in the gene encoding IL-1β were associated with higher serum IL-1β levels and liver fibrosis.
Conclusions

Male sex, age ≥18 years, elevated α-fetoprotein level, and HBeAg-negative hepatitis are risk factors for liver fibrosis. IL-1β is involved in the progression of liver fibrosis in subjects with HBeAg-negative hepatitis.

StephenW

儿童慢性乙型肝炎病毒感染的肝纤维化临床预测因子
吴家峰吴士锡宋志成李惠玲陈彦轩倪宏源许泽中吴美Chang畅
The Journal of Infectious Diseases，第217卷，第9期，2018年4月11日，第1408-1416页，https://doi.org/10.1093/infdis/jiy048
发布时间：
2018年1月30日

抽象
背景

本研究旨在阐明慢性乙型肝炎病毒（HBV）感染患者肝纤维化的预测因子。
方法

对533例慢性HBV感染患者（平均年龄±标准差，30.72±0.57岁）进行瞬态弹性成像以确定肝硬度。蛋白质阵列在用HBV突变体转染的Huh7细胞的血清样品和裂解物上进行;结果通过酶联免疫吸附测定来证实。慢性HBV感染伴肝纤维化和不伴肝纤维化的患者，检测白细胞介素1β（IL-1β）基因的单核苷酸多态性。
结果

男性，年龄≥18岁，血清甲胎蛋白水平> 3.6 ng / mL是≥7 kPa肝脏硬度测量的独立预测因子（分别为P = .005，.019和<.001）。 HBV e抗原（HBeAg）阴性肝炎与肝硬度增加相关（P <0.001）。在患有肝纤维化的受试者中证实了血清IL-1β水平的升高。在与HBeAg阴性肝炎相关的HBV突变体转染的Huh7细胞中IL-1β上调。编码IL-1β基因的rs16944处的AA基因型和rs1143627处的CC基因型与更高的血清IL-1β水平和肝纤维化相关。
结论

男性，年龄≥18岁，甲胎蛋白水平升高和HBeAg阴性肝炎是肝纤维化的危险因素。 IL-1β参与HBeAg阴性肝炎患者肝纤维化的进展。