慢性乙型肝炎患者尿液中检测到乙型肝炎病毒DNA的特征。

StephenW

BMC Gastroenterol. 2018 Mar 16;18(1):40. doi: 10.1186/s12876-018-0767-1.
Characterization of the hepatitis B virus DNA detected in urine of chronic hepatitis B patients.Jain S1, Su YH2, Su YP2, McCloud S1, Xue R3, Lee TJ2, Lin SC4, Lin SY5, Song W1,5, Steffen JD1,6, Hu CT7.
Author information
1JBS Science, Inc., Doylestown, PA, 18902, USA.2The Baruch S. Blumberg Institute, Doylestown, PA, 18902, USA.3The Second Hospital of Yuncheng, Yuncheng, Shanxi Province, 044000, China.4Buddhist Tzu Chi General Hospital and Tzu Chi University, 707, Sec. 3, Chung-Yang Rd, Hualien, 970, Taiwan, R.O.C.5U-Screen Dx Inc., Doylestown, PA, 18902, USA.6Hepron Molecular Lab, Inc., Doylestown, PA, 18902, USA.7Buddhist Tzu Chi General Hospital and Tzu Chi University, 707, Sec. 3, Chung-Yang Rd, Hualien, 970, Taiwan, R.O.C.. [email protected].

AbstractBACKGROUND: Detection of human hepatitis B virus (HBV) DNA in the urine of patients with chronic hepatitis B infection (CHB) has been reported previously, suggesting urine could provide a potential route of horizontal HBV transmission. However, it is not clear whether the HBV DNA detected in urine is indeed full-length, infectious viral DNA. The aim of this study is to assess the potential infectivity of urine from patients with CHB and to correlate HBV DNA detection in urine with clinical parameters, such as serum viral load and HBeAg status.
METHODS: Urine from 60 CHB patients with serum viral loads ranging from undetectable to 108 IU/mL were analyzed for HBV DNA and serum immune markers. HBV DNA was detected from total urine DNA and size-fractionated urine DNA (separated into ≤1 kb and > 1 kb fractions) by PCR analysis of six regions of the HBV genome.
RESULTS: Twenty-seven of 59 (45.7%) patients with HBV serum viral load (≥20 IU/mL) contained at least 20 copies per mL of fragmented HBV DNA in urine detected in at least 1 of the 6 PCR assay regions. Only one patient contained HBV DNA detected by all six regions, and was found to have evidence of blood in the urine. Sixteen of 25 urine samples with high viral load (> 105 IU/mL) and 11 of 34 urine samples with low viral load (< 105 IU/mL) contained detectable HBV DNA. Twelve of 27 (44.44%) patients with detectable HBV DNA in urine were HBeAg positive, and only 5 of these HBeAg positive patients were in the group of 33 (15.15%) patients with no detectable HBV DNA in urine. By Fishers' exact test, HBV DNA in urine is significantly associated with high serum viral load (P = 0.0197) and HBeAg (P = 0.0203).
CONCLUSIONS: We conclude that urine from CHB patients with healthy kidney function should not contain full-length HBV DNA, and therefore should not be infectious.


KEYWORDS: HBV DNA PCR assays; Hepatitis B virus; Hepatitis B virus DNA; Infection; Urine

PMID:29548283DOI:10.1186/s12876-018-0767-1

StephenW

BMC Gastroenterol。 2018年3月16日; 18（1）：40。 doi：10.1186 / s12876-018-0767-1。
慢性乙型肝炎患者尿液中检测到乙型肝炎病毒DNA的特征。
Jain S1，Su YH2，Su YP2，McCloud S1，Xue R3，Lee TJ2，Lin SC4，Lin SY5，Song W1,5，Steffen JD1,6，Hu CT7。
作者信息

1
JBS Science，Inc.，Doylestown，PA，18902，USA。
2
美国宾夕法尼亚州Doylestown的Baruch S. Blumberg研究所，18902年。
3
山西省运城市运城第二医院044000
4
佛教慈济综合医院和慈济大学，707，Sec。台湾970花莲中阳路3号
五
U-Screen Dx Inc.，Doylestown，PA，18902，USA。
6
Hepron Molecular Lab，Inc.，Doylestown，PA，18902，USA。
7
佛教慈济综合医院和慈济大学，707，Sec。台湾970花莲中阳路3号c。[email protected]。

抽象
背景：

之前有报道慢性乙型肝炎感染（CHB）患者尿液中检测到人类乙型肝炎病毒（HBV）DNA，提示尿液可能提供潜在的水平HBV传播途径。然而，目前尚不清楚尿液中检测到的HBV DNA是否为全长感染性病毒DNA。本研究的目的是评估CHB患者尿液的潜在传染性，并将尿液中HBV DNA检测与临床参数（如血清病毒载量和HBeAg状态）相关联。
方法：

对来自60名CHB患者的尿液进行HBV DNA和血清免疫标记物的分析，血清同步方法从不可检测到108IU / mL。通过对HBV基因组的六个区域进行PCR分析，从全部尿DNA和大小分级的尿DNA（分离为≤1kb和> 1Kb分数）中检测到HBV DNA。
结果：

在6个PCR检测区域中至少有1个检测到59个（45.7％）HBV血清病毒载量（≥20IU / mL）患者中有27个含有至少20个拷贝/ mL的尿液中每毫升片段化HBV DNA。只有一名患者含有全部六个区域检测到的HBV DNA，并且发现尿液中有血迹。高病毒载量（> 105 IU / mL）的25份尿液样本中的16份以及34份低病毒载量（<105 IU / mL）尿液样本中的11份含有可检测的HBV DNA。尿液中可检测到HBV DNA的27例患者中有12例（44.44％）为HBeAg阳性，33例（15.15％）尿液中未检测到HBV DNA的患者中仅有5例HBeAg阳性患者。通过Fishers的精确测试，尿液中的HBV DNA与高血清病毒载量（P = 0.0197）和HBeAg（P = 0.0203）显着相关。
结论：

我们得出结论，患有健康肾功能的CHB患者的尿液不应含有全长HBV DNA，因此不应该具有传染性。
关键词：

HBV DNA PCR测定;乙型肝炎病毒;乙型肝炎病毒DNA;感染;尿

结论：
29548283
DOI：
10.1186 / s12876-018-0767-1