差异表达的肝内基因有助于控制非活动载体阶段的乙型肝炎

StephenW

Differentially Expressed Intrahepatic Genes Contribute to Control of Hepatitis B Virus Replication in the Inactive Carrier Phase
Hongyan Liu Fahong Li Xiaoyong Zhang Jie Yu Jinyu Wang Jia Jia Xueping Yu Zhongliang Shen Zhenghong Yuan Xiaonan Zhang Zhanqing Zhang Xinxin Zhang Lungen Lu Hai Li Mengji Lu Jiming Zhang
The Journal of Infectious Diseases, Volume 217, Issue 7, 13 March 2018, Pages 1044–1054, https://doi.org/10.1093/infdis/jix683
Published:
02 January 2018

Abstract
Background

The natural history of chronic hepatitis B virus (HBV) infection was divided into 4 phases. Patients in the inactive carrier (IC) status and immune tolerant (IT) phase had normal alanine aminotransferase levels but huge different viral loads. The mechanism underlying low viral replication status in IC phase is unknown.
Methods

We determined the intrahepatic transcriptomes of 83 chronic hepatitis B patients by microarray analysis of liver biopsies, and screened the effect of differentially regulated genes on HBV replication using specific small interfering RNAs in vitro.
Results

The gene profile distinguishing active chronic hepatitis from IT and IC was predominantly composed of immune-related genes. The liver transcriptomes between the IT and IC phase were largely similar, and 109 expressed genes were significantly different. By performing systematic screening, 5 candidate genes including EVA1A, which were expressed at a relative higher level in IC phase than IT, were identified to regulate HBV replication and gene expression in cellular models.
Conclusions

The immune-related pathways were up-regulated in the active chronic hepatitis phase but not in the IT and IC phase. A number of intrahepatic genes highly expressed in the IC phase may participate in the control of HBV replication and determine the inactive status of HBV infection.
Issue Section:
Viruses
© The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: [email protected].

StephenW

差异表达的肝内基因有助于控制非活动载体阶段的乙型肝炎病毒复制
红岩刘发宏李晓勇张杰余金余王佳贾雪萍于忠良沉正宏袁晓楠张占庆张新新张伦根陆海李孟济陆继明张
传染病杂志，第217卷，第7期，2018年3月13日，第1044-1054页，https://doi.org/10.1093/infdis/jix683
发布时间：
2018年1月2日

抽象
背景

慢性乙型肝炎病毒（HBV）感染的自然史分为4个阶段。非活动载体（IC）状态和免疫耐受（IT）阶段的患者具有正常的丙氨酸转氨酶水平，但是巨大的不同病毒载量。 IC阶段低病毒复制状态的机制尚不清楚。
方法

我们通过肝活检的微阵列分析确定了83例慢性乙型肝炎患者的肝内转录组，并用体外特异性小干扰RNA筛选了差异调节基因对HBV复制的影响。
结果

区分活动性慢性肝炎与IT和IC的基因谱主要由免疫相关基因组成。 IT和IC阶段之间的肝转录组基本相似，并且109个表达基因显着不同。通过系统筛选，鉴定出5个候选基因，包括在IC阶段以比IT更高的水平表达的EVA1A，以在细胞模型中调节HBV复制和基因表达。
结论

免疫相关通路在活动性慢性肝炎阶段上调，但不在IT和IC阶段。一些在IC期高表达的肝内基因可能参与HBV复制的控制并确定HBV感染的非活动状态。
问题部分：
病毒
©The Author（s）2018.由牛津大学出版社出版的美国传染病协会出版。版权所有。有关权限，请发送电子邮件至：[email protected]。