在需要治疗的患者中与血清乙型肝炎病毒RNA水平相关的宿主

StephenW

Hepatology. 2018 Mar 7. doi: 10.1002/hep.29872. [Epub ahead of print]
Host and viral factors associated with serum hepatitis B virus RNA levels among patients in need for treatment.
Van Campenhout MJH1, van Bömmel F2, Pfefferkorn M2, Fischer J2, Deichsel D2, Boonstra A1, van Vuuren AJ1, Berg T2, Hansen BE1, 3, 4, Janssen HLA1, 4.
Author information

1
    Department of Gastroenterology and Hepatology, Erasmus MC University Medical Center, Rotterdam, The Netherlands.
2
    University Hospital Leipzig, Department of Gastroenterology and Rheumatology, Section of Hepatology, Leipzig, Germany.
3
    Institute of Health Policy, Management and Evaluation, University of Toronto.
4
    Toronto Center for Liver Disease, Toronto Western and General Hospital, University Health Network, Toronto, Canada.

Abstract

The use of RACE PCR technique (lower limit of detection 800 copies/ mL [c/mL]), serum HBV RNA levels were measured in samples of 488 untreated individuals with chronic HBV infection who were eligible to treatment according to currently used recommendations. We explored the association of serum levels of HBV RNA with patient and virus associated Factors. HBV genotype distribution was 21/10/20/46/3% for A/B/C/D/other. Mean HBV RNA serum level was 5.9 (1.6) log10 c/mL (HBeAg-positive CHB: 6.5 [1.2 ] log c/mL, HBeAg-negative CHB: 4.1 [1.2] log c/mL; p<0.001). By multivariable linear regression, factors associated with lower HBV RNA level were HBeAg-negativity (β=-0.69, p<0.001 ), HBV genotypes A (β=-0.13, p=0.002), B (β=-0.07, p=0.049) and C (β=-0.61, p<0.001) in comparison to D, and presence Of HBV basal core promoter mutation alone alone (β=-0.14, p=0.001) or in combination with precore mutation (β=-0.22, p<0.001). Higher serum ALT was associated with higher HBV RNA (β=0.23, p <0.001). HBV RNA staining strongly with HBV DNA (HBeAg-pos: r=0.72, p<0.001; HBeAg-neg: r=0.78, p<0.001), and moderately with qHBsAg (HBeAg-pos: r=0.54, p<0.001; HBeAg-neg: r=0.19, p=0.04) and qHBeAg (r=0.41, p<0.001).
CONCLUSION:

In this multi-ethnic cohort of 488 untreated individuals with chronic hepatitis B, factors associated with serum HBV RNA level were HBeAg status, serum ALT, HBV genotype, and presence of basal core promotor mutations. For the future use of serum HBV RNA as a Clinical marker it seems mandatory to take these factors in consideration. This article is protected by copyright. All rights reserved.

© 2018 by the American Association for the Study of Liver Diseases.
KEYWORDS:

HBV transcription; HBsAg loss; Serum marker; cccDNA;

PMID:
    29514389
DOI:
    10.1002/hep.29872

StephenW

肝病。 2018年3月7日doi：10.1002 / hep.29872。 [电子版提前打印]
在需要治疗的患者中与血清乙型肝炎病毒RNA水平相关的宿主和病毒因子。
Van Campenhout MJH1，vanBömmelF2，Pfefferkorn M2，Fischer J2，Deichsel D2，Boonstra A1，van Vuuren AJ1，Berg T2，Hansen BE1,3，Janssen HLA1,4。
作者信息

1
荷兰鹿特丹Erasmus MC大学医学中心消化内科和肝病科。
2
德国莱比锡莱比锡大学医院消化内科和风湿科，肝病科。
3
多伦多大学卫生政策，管理和评估研究所。
4
多伦多肝病研究中心，加拿大多伦多大学健康网络多伦多西部和综合医院。

抽象

根据目前使用的推荐，在488例未治疗的慢性HBV感染者中，使用RACE PCR技术（检测下限800拷贝/ mL [c / mL]），血清HBV RNA水平。我们探讨了血清HBV RNA水平与患者和病毒相关因素的关系。 A / B / C / D /其他HBV基因型分布为21/10/20/46/3％。平均HBV RNA血清水平为5.9（1.6）log10 c / mL（HBeAg阳性CHB：6.5 [1.2] log c / mL，HBeAg阴性CHB：4.1 [1.2] log c / mL; p <0.001）。通过多变量线性回归分析，HBV基因型A（β= -0.13，p = 0.002），B（β= -0.07，p = 0.049）和C（β= -0.61，p <0.001）与单独HBV单独存在（β= -0.14，p = 0.001）或与前核心突变（β= -0.22） ，p <0.001）。高血清ALT与更高的HBV RNA相关（β= 0.23，p <0.001）。乙肝病毒RNA强烈与HBV DNA染色（HBeAg-pos：r = 0.72，p <0.001; HBeAg阴性：r = 0.78，p <0.001），中度与qHBsAg（HBeAg-pos：r = 0.54，p <0.001; HBeAg阴性：r = 0.19，p = 0.04）和qHBeAg（r = 0.41，p <0.001）。
结论：

在这个多种族的488例慢性乙型肝炎未治疗个体中，与血清HBV RNA水平相关的因素有HBeAg状态，血清ALT，HBV基因型和基础核心启动子突变的存在。对于将来使用血清HBV RNA作为临床标记，似乎必须考虑这些因素。本文受版权保护。版权所有。

©美国肝病研究协会版权所有©2018年。
关键词：

HBV转录; HBsAg消失;血清标志物; cccDNA的;

结论：
29514389
DOI：
10.1002 / hep.29872