乙型肝炎病毒感染不会在肝脏诱导天然的免疫反应

StephenW

Brandon May
March 07, 2018
Hepatitis B Viral Infection Does Not Induce an Innate Immune Response in the Liver


Tissue samples were obtained by performing liver biopsies on 30 participants with chronic HBV infection and 42 control patients.

Although activation of toll-like receptor 3 (TLR3) binding and Sendai virus infection in the liver induces an innate immune response among those infected with hepatitis B virus (HBV), liver tissues of patients infected with HBV generally do not exhibit an active innate immune response, according to study findings published in Gastroenterology.

Investigators obtained liver biopsies from patients with chronic HBV infection (hepatitis B e antigen-positive or hepatitis B e antigen-negative, with or without hepatitis, n=30) and control patients (n=42), the majority of whom had fatty liver disease. The investigators used histology, immunohistochemistry, quantitative reverse-transcription polymerase chain reaction, in situ hybridization, HBV RNA quantification, and HBV genotyping for evaluating the liver tissues. In addition, the researchers also infected some of the specimens with Sendai virus or incubated with TLR ligands before assessment.

The liver samples from HBV-infected patients did not express greater interferon (IFN) or IFN-stimulated genes compared with specimens from the control group, suggesting HBV infection itself was not associated with an activation of an innate immune response. After activation of TLR3 with poly(I:C) or Sendai virus infections, liver specimens of HBV-infected patients produced IFN and expressed IFN-stimulated genes, indicating that the innate immune response is not suppressed in liver tissue.


Because the investigators were unable to collect liver specimens from patients with acute HBV infection, the study was unable to determine whether active suppression of IFN-inducing sensory pathways or IFN signaling occurs in the early phases of HBV.

Findings from this study "support the hypothesis that HBV behaves like a stealth virus by staying under the radar of the pathogen detection system."
Reference

Suslov A, Boldanova T, Wang X, Wieland S, Heim MH. Hepatitis B virus does not interfere with innate immune responses in the human liver [published online February 2, 2018]. Gastroenterology. doi:10.1053/j.gastro.2018.01.034

StephenW

布兰登梅
2018年3月7日
乙型肝炎病毒感染不会在肝脏诱导天然的免疫反应


通过对30名患有慢性HBV感染的患者和42名对照患者进行肝活组织检查获得组织样品。

虽然肝脏中Toll样受体3（TLR3）结合和仙台病毒感染的激活诱导感染乙型肝炎病毒（HBV）的那些人中的先天性免疫应答，但是感染HBV的患者的肝组织通常不表现出活跃的先天免疫根据发表在胃肠病学的研究结果，

研究人员从慢性HBV感染患者（乙型肝炎e抗原阳性或乙型肝炎e抗原阴性，伴或不伴肝炎，n = 30）和对照患者（n = 42）中获得肝活检，其中大多数患者患有脂肪肝疾病。研究人员使用组织学，免疫组织化学，定量逆转录聚合酶链式反应，原位杂交，HBV RNA定量和用于评估肝组织的HBV基因分型。另外，研究人员还在评估前用仙台病毒感染了一些标本或与TLR配体孵育。

与对照组相比，来自HBV感染患者的肝脏样本不表达更大的干扰素（IFN）或IFN刺激的基因，提示HBV感染本身与先天性免疫应答的激活无关。用聚（I：C）或仙台病毒感染激活TLR3后，HBV感染患者的肝脏样本产生IFN并表达IFN-刺激的基因，表明先天免疫反应在肝组织中未被抑制。


由于研究人员无法从急性HBV感染患者采集肝脏标本，因此该研究无法确定是否在HBV的早期阶段主动抑制IFN诱导的感觉通路或IFN信号传导。

这项研究的结果“支持这样的假设，即HBV一直处于病原体检测系统的雷达下，就像隐形病毒一样。”
参考

Suslov A，Boldanova T，Wang X，Wieland S，Heim MH。乙型肝炎病毒不会干扰人类肝脏中的先天免疫反应[2018年2月2日在线发表]。消化内科。 DOI：10.1053 / j.gastro.2018.01.034