循环调节性Tfh细胞在慢性乙型肝炎感染患者中富集并诱导调

StephenW

Exp Cell Res. 2018 Feb 28. pii: S0014-4827(18)30115-0. doi: 10.1016/j.yexcr.2018.02.031. [Epub ahead of print]
Circulating regulatory Tfh cells are enriched in patients with chronic hepatitis B infection and induce the differentiation of regulatory B cells.Wang R1, Xie R2, Song Z3.
Author information
1Department of Infection, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan Province, China.2Department of Respiration，Yellow River Central Hospital, Zhengzhou, Henan Province, China.3Department of Hematology & Oncology, the 155th Central Hospital of PLA/The Key Laboratory of Hematology of Kaifeng City, Kaifeng, Henan Province, China. Electronic address: [email protected].

AbstractChronic hepatitis B virus (HBV) infection is a complex disease with dysregulations in the immune system. Follicular helper T (Tfh) cells are professional B helper cells that are crucial to the development of antibody responses and are involved in a variety of diseases. In this study, we examined the circulating Tfh cells in patients with chronic HBV infection. We observed that CD3+CD4+CXCR5+ circulating Tfh cells contained a CD25+Foxp3+ Treg-like subset that was significantly enriched in patients with chronic HBV infections. The CD25+ Tfh subset presented distinctive cytokine secretion profile, such as lower interferon (IFN)-γ and interleukin (IL)-17, and higher transforming growth factor (TGF)-β secretion, compared to the CD25- Tfh subset. When incubated with autologous naive CD10-CD27-CD19+ B cells, the CD25+ Tfh subset was less capable of mediating CD20-/loCD38+ plasmablast differentiation than the CD25- Tfh subset. In terms of Ig production, CD25+ Tfh cells were more potent at inducing IgM but less potent at inducing IgG and IgA than CD25- Tfh cells. Interestingly, B cells following incubation with CD25+ Tfh cells presented elevated regulatory function, with higher production of IL-10 and enhanced capacity of suppressing autologous CD8+ T cell inflammation. In the chronic HBV-infected patients, the frequency of IL-10+ B cells and the HBV viral load were positively correlated with the frequency of CD25+Foxp3+ CD4+CXCR5+ Tfh cells. Together, this study presented that CD25+Foxp3+ Treg-like Tfh cells were enriched in chronic HBV-infected patients and could promote regulatory B cell functions.


KEYWORDS: B cell; Breg; CXCR5; HBV; Tfh

PMID:29501568DOI:10.1016/j.yexcr.2018.02.031

StephenW

Exp Cell Res。 2018年2月28日。pii：S0014-4827（18）30115-0。 doi：10.1016 / j.yexcr.2018.02.031。 [电子版提前打印]
循环调节性Tfh细胞在慢性乙型肝炎感染患者中富集并诱导调节性B细胞的分化。
王R1，谢R2，宋Z3。
作者信息

1
    郑州大学附属郑州中心医院感染科，河南郑州。
2
    河南郑州黄河中心医院呼吸科。
3
    中国人民解放军第155中心医院血液肿瘤科/开封市血液病重点实验室，河南开封市。电子地址：[email protected]。

抽象

慢性乙型肝炎病毒（HBV）感染是一种复杂的疾病，在免疫系统中存在调节异常。滤泡辅助T（Tfh）细胞是对抗体应答发展至关重要并且参与多种疾病的专业B辅助细胞。在这项研究中，我们检测了慢性HBV感染患者中的循环Tfh细胞。我们观察到CD3 + CD4 + CXCR5 +循环Tfh细胞含有CD25 + Foxp3 + Treg样亚组，其在慢性HBV感染患者中显着富集。与CD25-Tfh子集相比，CD25 + Tfh子集呈现独特的细胞因子分泌谱，例如较低的干扰素（IFN）-γ和白细胞介素（IL）-17以及较高的转化生长因子（TGF）-β分泌。当与自体幼稚CD10-CD27-CD19 + B细胞一起孵育时，CD25 + Tfh子集与CD25-Tfh子集相比能够介导CD20- / loCD38 +浆母细胞分化的能力较弱。就Ig产生而言，CD25 + Tfh细胞在诱导IgM方面更有效，但比CD25-Tfh细胞诱导IgG和IgA时更有效。有趣的是，与CD25 + Tfh细胞孵育后的B细胞表现出升高的调节功能，具有更高的IL-10产生和增强的抑制自体CD8 + T细胞炎症的能力。慢性HBV感染者中，IL-10 + B细胞频率和HBV病毒载量与CD25 + Foxp3 + CD4 + CXCR5 + Tfh细胞频率呈正相关。总之，这项研究表明CD25 + Foxp3 + Treg样Tfh细胞在慢性HBV感染患者中富集，并且可以促进调节性B细胞功能。
关键词：

B细胞; BREG; CXCR5; HBV; TFH

结论：
    29501568
DOI：
    10.1016 / j.yexcr.2018.02.031