新型稳定的HBV产生细胞系，用于在人肝癌细胞系中表达和筛

StephenW

Biochem Biophys Res Commun. 2018 Feb 23. pii: S0006-291X(18)30415-7. doi: 10.1016/j.bbrc.2018.02.175. [Epub ahead of print]
Novel stable HBV producing cell line systems for expression and screening antiviral inhibitor of hepatitis B virus in human hepatoma cell line.Ogura N1, Ogawa K2, Watashi K3, Ito T4, Wakita T5.
Author information
1Central Pharmaceutical Research Institute, Japan Tobacco Inc, Osaka, 569-1125, Japan. Electronic address: [email protected].2Central Pharmaceutical Research Institute, Japan Tobacco Inc, Osaka, 569-1125, Japan. Electronic address: [email protected].3Department of Virology II, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan. Electronic address: [email protected].4Digestive Diseases Center, Showa University Koto-Toyosu Hospital, Tokyo, 135-8577, Japan. Electronic address: [email protected].5Department of Virology II, National Institute of Infectious Diseases, Tokyo, 162-8640, Japan. Electronic address: [email protected].

AbstractChronic hepatitis B virus (HBV) infection is currently a major public health burden. Therefore, there is an urgent need for the development of novel antiviral inhibitors. The stable HBV-producing cell lines of genotype D are widely used to investigate the HBV life cycle and to evaluate antiviral agents. However, stable HBV-producing cell lines of different genotypes do not exist. To construct more convenient and efficient novel cell systems, stable cell lines of genotypes A, B, and C were established using a full-length HBV genome sequence isolated from chronic HBV patients in human hepatoma HepG2 cells. Novel HBV clones were identified and stable HBV-producing cell lines derived from these clones were constructed. HBV replication activities demonstrated time-dependent expression, and the novel cell lines were susceptible to several antiviral inhibitors with no cytotoxicity. Furthermore, infectious viruses were produced from these cell lines. In conclusion, we have established novel stable HBV-producing cell line systems of genotypes A, B, and C. These systems can provide valuable tools for screening antiviral agents and analyzing viral phenotypes in vitro.


KEYWORDS: Antiviral inhibitor; Genotype; Hepatitis B virus; Novel stable cell line

PMID:29481805DOI:10.1016/j.bbrc.2018.02.175

StephenW

Biochem Biophys Res Commun。 2018年2月23日。pii：S0006-291X（18）30415-7。 doi：10.1016 / j.bbrc.2018.02.175。 [电子版提前打印]
新型稳定的HBV产生细胞系，用于在人肝癌细胞系中表达和筛选乙型肝炎病毒的抗病毒抑制剂。
Ogura N1，Ogawa K2，Watashi K3，Ito T4，Wakita T5。
作者信息

1
    中央药物研究所，日本烟草公司，大阪，569-1125，日本。电子地址：[email protected]。
2
    中央药物研究所，日本烟草公司，大阪，569-1125，日本。电子地址：[email protected]。
3
    日本东京国立传染病研究所病毒学系II，162-8640。电子地址：[email protected]。
4
    东京昭和大学江东丰子医院消化疾病中心，日本135-8577。电子地址：[email protected]。
五
    日本东京国立传染病研究所病毒学系II，162-8640。电子地址：[email protected]。

抽象

慢性乙型肝炎病毒（HBV）感染目前是公共健康的主要负担。因此，迫切需要开发新的抗病毒抑制剂。基因型D稳定的HBV产生细胞系广泛用于研究HBV的生命周期并评估抗病毒药物。然而，不存在不同基因型的稳定的HBV产生细胞系。为了构建更加方便和有效的新型细胞系统，使用从慢性HBV患者分离的全长HBV基因组序列在人肝细胞瘤HepG2细胞中建立了基因型A，B和C的稳定细胞系。鉴定出新的HBV克隆并构建了源自这些克隆的稳定的产HBV细胞系。 HBV复制活动表现出时间依赖性表达，并且新细胞系对几种抗细胞毒性抑制剂敏感，没有细胞毒性。此外，从这些细胞系产生感染性病毒。总之，我们已经建立了基因型A，B和C的新型稳定的HBV产生细胞系系统。这些系统可以提供筛选抗病毒药物和分析体外病毒表型的有价值的工具。
关键词：

抗病毒抑制剂;基因型;乙型肝炎病毒;新型稳定细胞系

结论：
    29481805
DOI：
    10.1016 / j.bbrc.2018.02.175