中国汉族人群Toll样受体基因多态性与乙型肝炎病毒活性的相

StephenW

BMC Infect Dis. 2018 Jan 10;18(1):28. doi: 10.1186/s12879-018-2943-x.
Correlation between polymorphisms in toll-like receptor genes and the activity of hepatitis B virus among treatment-naïve patients: a case-control study in a Han Chinese population.
Lin Y1,2, Gao ZX1,2, Shen X1,3, Chen MJ1, Li YT1, Li SL4, Lin HL4, Zhao QF1,3, Liu F1,2, Niu JJ5,6.
Author information

1
    Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen University, 201-209 Hubin South Road, Siming District, Xiamen City, 361004, Fujian Province, People's Republic of China.
2
    Institute of Infectious Disease, Medical College of Xiamen University, Xiamen, Fujian Province, China.
3
    School of Public Health, Fujian Medical University, Fuzhou, Fujian Province, China.
4
    Xiamen Huli District Maternity and Child Care Hospital, Xiamen, Fujian Province, China.
5
    Center of Clinical Laboratory, Zhongshan Hospital, Medical College of Xiamen University, 201-209 Hubin South Road, Siming District, Xiamen City, 361004, Fujian Province, People's Republic of China. [email protected].
6
    Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma, Xiamen, Fujian Province, China. [email protected].

Abstract
BACKGROUND:

Because of the high prevalence and absence of cure for infection, chronic hepatitis B virus (HBV) infection has been acknowledged as a pressing public health issue. Toll-like receptors (TLRs) activate the human innate immune system and the polymorphisms in TLRs may alter their function. The present study aimed to investigate the association between TLR polymorphisms and disease progression of chronic HBV infection.
METHODS:

During the study period, 211 treatment-naïve patients with chronic HBV infection were recruited, and blood samples were collected from each individual. Matrix-assisted laser desorption/ionization time of flight mass spectrometry was employed to genotype the selected TLR polymorphisms after human genome extraction. In addition, HbsAg, TNF-α, and IL-6 levels were quantified using enzyme linked immunosorbent assay (ELISA). Statistical analyses were conducted to investigate the association between TLR polymorphisms and hepatitis activity, liver function parameters, HbsAg level, and cytokine level.
RESULTS:

We did not observe any mutations in rs4986790, rs4986791, and rs5743708 among all study subjects. A logistic regression revealed that mutations in rs3804099 and rs4696480 were associated with milder hepatitis activity. Consistent with the logistic regression, improved liver function parameters and reduced level of both HbsAg and cytokines were also correlated with the mutant carriers of rs3804099 and rs4696480.
CONCLUSIONS:

TLR mutations were significantly associated with milder hepatitis activity among patients with chronic HBV infection. Therefore, we conclude that the activation of TLR pathways may further intensify the inflammation of hepatocytes, and leads to progression of disease.
KEYWORDS:

Hepatitis B virus; Hepatitis activity; Immune response; Toll-like receptors

PMID:
    29320990
DOI:
    10.1186/s12879-018-2943-x

StephenW

BMC传染病2018年1月10日; 18（1）：28。 doi：10.1186 / s12879-018-2943-x。
中国汉族人群Toll样受体基因多态性与乙型肝炎病毒活性的相关性研究
林1 1,2，高志新1,2，沉茜1，陈梅娟1，李玉涛1，李SL 4，林红4，赵启飞1,3，刘飞1,2，牛俊杰5,6。
作者信息

1
    厦门大学医学院中山医院检验中心，福建省厦门市思明区湖滨南路201-209号，邮编：361004
2
    厦门大学医学院传染病研究所，福建厦门
3
    福建医科大学公共卫生学院，福建福州。
4
    厦门湖里区妇幼保健院，中国福建省厦门市。
五
    厦门大学医学院中山医院检验中心，福建省厦门市思明区湖滨南路201-209号，邮编：361004 [email protected]。
6
    福建省慢性肝病肝癌重点实验室，福建省厦门市。 [email protected]。

抽象
背景：

慢性乙型肝炎病毒（HBV）感染由于感染率高且无法治愈，已被公认为是一个迫切的公共卫生问题。 Toll样受体（TLRs）激活人类先天性免疫系统，TLR中的多态性可能改变其功能。本研究旨在探讨TLR多态性与慢性HBV感染疾病进展之间的关系。
方法：

在研究期间，招募了211名慢性HBV感染的初治患者，并且从每个个体收集血液样品。基质辅助激光解吸/电离飞行时间质谱法被用来基因型选择人类基因组提取后的TLR多态性。另外，使用酶联免疫吸附测定（ELISA）定量HbsAg，TNF-α和IL-6水平。进行统计分析以研究TLR多态性与肝炎活性，肝功能参数，HbsAg水平和细胞因子水平之间的关联。
结果：

在所有研究对象中，我们没有观察到rs4986790，rs4986791和rs5743708的任何突变。逻辑回归显示rs3804099和rs4696480的突变与肝炎活动较轻相关。与逻辑回归一致，肝功能指标改善，HbsAg和细胞因子水平降低与rs3804099和rs4696480突变携带者相关。
结论：

慢性HBV感染患者中TLR突变与肝炎活动轻度相关。因此，我们认为TLR通路的激活可能进一步加剧肝细胞的炎症反应，导致疾病进展。
关键词：

乙型肝炎病毒;肝炎活动;免疫反应; Toll样受体

结论：
    29320990
DOI：
    10.1186 / s12879-018-2943-X