英国利兹大学的研究人员发现了复RNA信号促进了核衣壳的组hb

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https://www.id-hub.com/2017/06/2 ... leocapsid-assembly/

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HBV RNA pre-genome encodes specific motifs that mediate interactions with the viral core protein that promote nucleocapsid assembly
Nikesh Patel, Simon J. White, Rebecca F. Thompson, Richard Bingham, Eva U. Weiß, Daniel P. Maskell, Adam Zlotnick, Eric C. Dykeman, Roman Tuma, Reidun Twarock, Neil A. Ranson & Peter G. Stockley
Nature Microbiology 2, Article number: 17098 (2017)
doi:10.1038/nmicrobiol.2017.98
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Cryoelectron microscopyHepatitis B virusVirus structures
Received:
18 August 2016
Accepted:
17 May 2017
Published online:
19 June 2017
Abstract
Formation of the hepatitis B virus nucleocapsid is an essential step in the viral lifecycle, but its assembly is not fully understood. We report the discovery of sequence-specific interactions between the viral pre-genome and the hepatitis B core protein that play roles in defining the nucleocapsid assembly pathway. Using RNA SELEX and bioinformatics, we identified multiple regions in the pre-genomic RNA with high affinity for core protein dimers. These RNAs form stem-loops with a conserved loop motif that trigger sequence-specific assembly of virus-like particles (VLPs) at much higher fidelity and yield than in the absence of RNA. The RNA oligos do not interact with preformed RNA-free VLPs, so their effects must occur during particle assembly. Asymmetric cryo-electron microscopy reconstruction of the T = 4 VLPs assembled in the presence of one of the RNAs reveals a unique internal feature connected to the main core protein shell via lobes of density. Biophysical assays suggest that this is a complex involving several RNA oligos interacting with the C-terminal arginine-rich domains of core protein. These core protein–RNA contacts may play one or more roles in regulating the organization of the pre-genome during nucleocapsid assembly, facilitating subsequent reverse transcription and acting as a nucleation complex for nucleocapsid assembly.

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HBV RNA前基因组编码了特定的基序，介导与病毒核心蛋白的相互作用，从而促进核衣壳的组装
Nikesh Patel,西蒙·j .白色,丽贝卡·f·汤普森,理查德·宾厄姆Eva Weiß,丹尼尔·p·Maskell亚当Zlotnick,埃里克·c·戴克曼罗马Tuma Reidun Twarock,尼尔·a·Ranson &彼得g . Stockley
《自然微生物学2》第17098号(2017年)
doi:10.1038 / nmicrobiol.2017.98
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低温电子显微镜下的病毒结构
收到:
2016年8月18日
接受:
2017年5月17日
网上发表:
2017年6月19日
抽象的
乙型肝炎病毒核衣壳的形成是病毒生命周期中的一个重要步骤，但它的组装尚未完全被了解。我们报告了病毒预基因组与在定义核衣壳组装途径中起作用的乙型肝炎核心蛋白之间的序列特异性相互作用的发现。利用RNA SELEX和生物信息学，我们发现了具有高亲和力的核心蛋白二聚体的前基因组RNA的多个区域。这些RNA形成了茎环，带有一个保守的环状结构，它能在比没有RNA的情况下，以更高的保真度和产量触发序列特异性的病毒样粒子(VLPs)。RNA寡聚体不与预先形成的RNA -free VLPs相互作用，因此它们的作用必须发生在粒子装配过程中。在一种rna的存在下组装的T = 4 VLPs的非对称低温电子显微镜重建揭示了一种独特的内部特征，它通过密度的裂片连接到主要的核心蛋白外壳上。生物物理分析表明，这是一个复杂的涉及多个RNA寡核蛋白与c端精氨酸丰富的核心蛋白质领域的相互作用。这些核心蛋白- rna接触在核衣壳组装过程中可能扮演一个或多个角色来调节预基因组的组织，促进随后的反向转录，并作为核衣壳组装的成核联合体。