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Chronic inflammation-elicited liver progenitor cell conversion to liver cancer stem cell with clinical significance
Xiao-Feng Li1,†, Cheng Chen1,2,†, Dai-Min Xiang1,3,†, Le Qu1,2,†, Wen Sun1, Xin-Yuan Lu4, Teng-Fei Zhou1, Shu-Zhen Chen1, Bei-Fang Ning5, Zhuo Cheng1, Ming-Yang Xia1, Wei-Feng Shen6, Wen Yang1, Wen Wen1, Terence Kin Wah Lee7, Wen-Ming Cong4, Hong-Yang Wang1,3,* andJin Ding1,3,*
Version of Record online: 6 NOV 2017
DOI: 10.1002/hep.29372
© 2017 by the American Association for the Study of Liver Diseases.
Hepatology
Volume 66, Issue 6, pages 1934–1951, December 2017
Article has an altmetric score of 1
1 The International Cooperation Laboratory on Signal Transduction, Eastern Hepatobiliary Surgery Hospital, The Second Military Medical University, Shanghai, China
2 Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China
3 National Center of Liver Cancer, Shanghai, China
4 Department of Pathology, Eastern Hepatobiliary Surgery Hospital, Shanghai, China
5 Department of Gastroenterology, Changzheng Hospital, Shanghai, China
6 Department of Hepatic Surgery, Eastern Hepatobiliary Surgery Hospital, Shanghai, China
7 Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong
† These authors have contributed equally to this work.
Email: Hong-Yang Wang ([email protected]), Jin Ding ([email protected])
*ADDRESS CORRESPONDENCE AND REPRINT REQUESTS TO:
Hongyang Wang, M.D., Ph.D., and Jin Ding, M.D., Ph.D.
225 Changhai Road
Shanghai, 200438, China
E-mail: [email protected] and [email protected]
Tel: +86-21-81875361
Potential conflict of interest: Nothing to report.
Supported by grants from the National Key R&D Program of China (2017YFA0504503) and the National Natural Science Foundation of China (81572897, 81372329, and 81572412).
The substantial heterogeneity and hierarchical organization in liver cancer support the theory of liver cancer stem cells (LCSCs). However, the relationship between chronic hepatic inflammation and LCSC generation remains obscure. Here, we observed a close correlation between aggravated inflammation and liver progenitor cell (LPC) propagation in the cirrhotic liver of rats exposed to diethylnitrosamine. LPCs isolated from the rat cirrhotic liver initiated subcutaneous liver cancers in nonobese diabetic/severe combined immunodeficient mice, suggesting the malignant transformation of LPCs toward LCSCs. Interestingly, depletion of Kupffer cells in vivo attenuated the LCSC properties of transformed LPCs and suppressed cytokeratin 19/Oval cell 6–positive tumor occurrence. Conversely, LPCs cocultured with macrophages exhibited enhanced LCSC properties. We further demonstrated that macrophage-secreted tumor necrosis factor-α triggered chromosomal instability in LPCs through the deregulation of ubiquitin D and checkpoint kinase 2 and enhanced the self-renewal of LPCs through the tumor necrosis factor receptor 1/Src/signal transducer and activator of transcription 3 pathway, which synergistically contributed to the conversion of LPCs to LCSCs. Clinical investigation revealed that cytokeratin 19/Oval cell 6–positive liver cancer patients displayed a worse prognosis and exhibited superior response to sorafenib treatment. Conclusion: Our results not only clarify the cellular and molecular mechanisms underlying the inflammation-mediated LCSC generation but also provide a molecular classification for the individualized treatment of liver cancer. (Hepatology 2017;66:1934–1951)
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发表于 2017-11-23 15:19