Genetic variation of hepatitis B surface antigen coding region among infants w

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Med Virol 2002 Nov;68(3):319-27


Genetic variation of hepatitis B surface antigen coding region among infants with chronic hepatitis B virus infection.

Nainan OV, Khristova ML, Byun K, Xia G, Taylor PE, Stevens CE, Margolis HS
Division of Viral Hepatitis (World Health Organization Collaborating Center
for Research and Reference in Viral Hepatitis), National Center for
Infectious Diseases, Centers for Disease Control and Prevention, Atlanta,
Georgia.

[Medline record in process]


Variants in the amino acid composition of the primary antibody-binding site
of hepatitis B surface antigen (HBsAg) have been identified in a number of
populations with chronic hepatitis B virus (HBV) infection. Direct
sequencing of amplified or cloned PCR products, solid phase detection of
sequence-specific PCR products (SP-PCR), and limiting dilution cloning PCR
(LDC-PCR) were compared to determine their sensitivity in detecting
differing concentrations of HBsAg variants. LDC-PCR had the greatest
sensitivity and could detect HBsAg variants at a concentration of 0.1% of
the total viral population. HBsAg variants were detected in 51% of infants
with chronic HBV infection acquired after postexposure prophylaxis, and more than half of the variants were detected only by the most sensitive methods.
J. Med. Virol. 68:319-327, 2002. Copyright 2002 Wiley-Liss, Inc.

PMID: 12226817, UI: 22213615