使用完全定量纤维化评估工具，不同病因的肝纤维化特征。

StephenW

Braz J Med Biol Res. 2017 May 18;50(6):e5234. doi: 10.1590/1414-431X20175234.
Characteristics of liver fibrosis with different etiologies using a fully quantitative fibrosis assessment tool.
Wu Q1,2, Zhao X1,2, You H1,2.
Author information

1    Beijing Key Laboratory of Translational Medicine in Liver Cirrhosis, Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
2    National Clinical Research Center for Digestive Diseases, Beijing, China.

Abstract

This study aimed to test the diagnostic performance of a fully quantitative fibrosis assessment tool for liver fibrosis in patients with chronic hepatitis B (CHB), primary biliary cirrhosis (PBC) and non-alcoholic steatohepatitis (NASH). A total of 117 patients with liver fibrosis were included in this study, including 50 patients with CHB, 49 patients with PBC and 18 patients with NASH. All patients underwent liver biopsy (LB). Fibrosis stages were assessed by two experienced pathologists. Histopathological images of LB slices were processed by second harmonic generation (SHG)/two-photon excited fluorescence (TPEF) microscopy without staining, a system called qFibrosis (quantitative fibrosis) system. Altogether 101 quantitative features of the SHG/TPEF images were acquired. The parameters of aggregated collagen in portal, septal and fibrillar areas increased significantly with stages of liver fibrosis in PBC and CHB (P<0.05), but the same was not found for parameters of distributed collagen (P>0.05). There was a significant correlation between parameters of aggregated collagen in portal, septal and fibrillar areas and stages of liver fibrosis from CHB and PBC (P<0.05), but no correlation was found between the distributed collagen parameters and the stages of liver fibrosis from those patients (P>0.05). There was no significant correlation between NASH parameters and stages of fibrosis (P>0.05). For CHB and PBC patients, the highest correlation was between septal parameters and fibrosis stages, the second highest was between portal parameters and fibrosis stages and the lowest correlation was between fibrillar parameters and fibrosis stages. The correlation between the septal parameters of the PBC and stages is significantly higher than the parameters of the other two areas (P<0.05). The qFibrosis candidate parameters based on CHB were also applicable for quantitative analysis of liver fibrosis in PBC patients. Different parameters should be selected for liver fibrosis assessment in different stages of PBC compared with CHB.

PMID:
    28538834
DOI:
    10.1590/1414-431X20175234

StephenW

Braz J Med Biol Res。 2017年5月18日; 50（6）：e5234。 doi：10.1590 / 1414-431X20175234。
使用完全定量纤维化评估工具，不同病因的肝纤维化特征。
吴Q1,2，赵X1,2，你H1,2。
作者信息

1北京市第一医科大学北京友谊医院肝脏肝硬化转化医学北京市重点实验室，北京。
2国家消化疾病临床研究中心，北京，中国。

抽象

本研究旨在测试慢性乙型肝炎（CHB），原发性胆汁性肝硬化（PBC）和非酒精性脂肪性肝炎（NASH）患者肝纤维化完全定量纤维化评估工具的诊断性能。本研究共纳入117例肝纤维化患者，其中CHB患者50例，PBC 49例，NASH 18例。所有患者均行肝活检（LB）。两名经验丰富的病理学家评估纤维化阶段。通过二次谐波生成（SHG）/双光子激发荧光（TPEF）显微镜无染色，称为qFibrosis（定量纤维化）系统的系统处理LB切片的组织病理学图像。共获得了SHG / TPEF图像的101个定量特征。门静脉，间隔和纤维区聚集的胶原蛋白参数在PBC和CHB的肝纤维化阶段显着升高（P <0.05），但分布型胶原参数没有发现（P> 0.05）。来自CHB和PBC的门静脉，间隔和纤维性区域的聚集胶原蛋白参与和肝纤维化阶段之间存在显着相关性（P <0.05），但分布胶原参数与肝纤维化阶段之间无相关性患者（P> 0.05）。 NASH参数与纤维化阶段无显着相关性（P> 0.05）。对于CHB和PBC患者，间隔参数和纤维化阶段之间的相关性最高，门静脉参数和纤维化阶段之间的最高相关性，纤维性参数和纤维化阶段之间的相关性最低。 PBC和各期间隔参数之间的相关性明显高于其他两个区域的参数（P <0.05）。基于CHB的qFibrosis候选参数也适用于PBC患者肝纤维化的定量分析。与CHB相比，PBC不同阶段的肝纤维化评估参数应不同。

结论：
    28538834
DOI：
    10.1590 / 1414-431X20175234