完整和不完整的乙型肝炎病毒颗粒：形成，功能和应用。

StephenW

Viruses. 2017 Mar 21;9(3). pii: E56. doi: 10.3390/v9030056.
Complete and Incomplete Hepatitis B Virus Particles: Formation, Function, and Application.Hu J1, Liu K2,3.
Author information
1Department of Microbiology and Immunology, Penn State University College of Medicine, Hershey, PA 17033, USA. [email protected].2Department of Microbiology and Immunology, Penn State University College of Medicine, Hershey, PA 17033, USA. [email protected].3College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China. [email protected].

AbstractHepatitis B virus (HBV) is a para-retrovirus or retroid virus that contains a double-stranded DNA genome and replicates this DNA via reverse transcription of a RNA pregenome. Viral reverse transcription takes place within a capsid upon packaging of the RNA and the viral reverse transcriptase. A major characteristic of HBV replication is the selection of capsids containing the double-stranded DNA, but not those containing the RNA or the single-stranded DNA replication intermediate, for envelopment during virion secretion. The complete HBV virion particles thus contain an outer envelope, studded with viral envelope proteins, that encloses the capsid, which, in turn, encapsidates the double-stranded DNA genome. Furthermore, HBV morphogenesis is characterized by the release of subviral particles that are several orders of magnitude more abundant than the complete virions. One class of subviral particles are the classical surface antigen particles (Australian antigen) that contain only the viral envelope proteins, whereas the more recently discovered genome-free (empty) virions contain both the envelope and capsid but no genome. In addition, recent evidence suggests that low levels of RNA-containing particles may be released, after all. We will summarize what is currently known about how the complete and incomplete HBV particles are assembled. We will discuss briefly the functions of the subviral particles, which remain largely unknown. Finally, we will explore the utility of the subviral particles, particularly, the potential of empty virions and putative RNA virions as diagnostic markers and the potential of empty virons as a vaccine candidate.


KEYWORDS: Australian antigen; CCC DNA; HBcAg; HBsAg; diagnosis; empty virion; hepatitis B virus; subviral particles; vaccine; virion

PMID:28335554DOI:10.3390/v9030056

StephenW

病毒2017年3月21日; 9（3）。 pii：E56。 doi：10.3390 / v9030056。
完整和不完整的乙型肝炎病毒颗粒：形成，功能和应用。
胡杰1，刘K2 3。
作者信息

1
    美国宾夕法尼亚州立大学医学院微生物与免疫学系，美国宾夕法尼亚州赫尔案17033号。 [email protected]
2
    美国宾夕法尼亚州立大学医学院微生物与免疫学系，美国宾夕法尼亚州赫尔案17033号。 [email protected]
3
    浙江科技大学生命科学学院，杭州310018。 [email protected]

抽象

乙型肝炎病毒（HBV）是一种副反转录病毒或网状病毒，含有双链DNA基因组，并通过RNA pregenome的逆转录复制该DNA。病毒逆转录在RNA和病毒逆转录酶包装后在衣壳内进行。 HBV复制的主要特征是选择含有双链DNA的衣壳，但不包含含有RNA或单链DNA复制中间体的衣壳，用于在病毒体分泌过程中包膜。因此，完整的HBV病毒粒子包含外壳，其上散布有病毒包膜蛋白，其包围衣壳，其反过来包裹双链DNA基因组。此外，HBV形态发生的特征在于释放比完整病毒体更多数量级的亚病毒颗粒。一类亚病毒颗粒是仅含有病毒包膜蛋白的经典表面抗原颗粒（澳大利亚抗原），而最近发现的无基因组（空）病毒粒子包含包膜和衣壳，但不含基因组。此外，最近的证据表明，低含量的含RNA颗粒可能会被释放。我们将总结目前已知的完整和不完整的HBV颗粒如何组装。我们将简要讨论亚病毒颗粒的功能，这些功能在很大程度上是未知的。最后，我们将探讨亚病毒颗粒的用途，特别是潜在的空载体病毒粒子和推定的RNA病毒粒子作为诊断标记物，以及潜在的空白病毒作为候选疫苗。
关键词：

澳大利亚抗原CCC DNA; HBcAg; HBsAg;诊断;空病毒粒子;乙型肝炎病毒;亚病毒颗粒疫苗;病毒粒子

PMID：
    28335554
DOI：
    10.3390 / v9030056