血清乙型肝炎核心相关抗原是肝内共价闭合环形DNA在慢性乙

StephenW

Sci Rep. 2017 Dec;7(1):173. doi: 10.1038/s41598-017-00111-0. Epub  2017 Mar 14.
Serum hepatitis B core-related antigen is a satisfactory surrogate marker of intrahepatic covalently closed circular DNA in chronic hepatitis B.Chen EQ1,2, Feng S3, Wang ML1,2, Liang LB1,2, Zhou LY1,2, Du LY1,2, Yan LB1,2, Tao CM3, Tang H4,5.
Author information
1Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, P.R. China.2Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, P.R. China.3Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, P.R. China.4Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, P.R. China. [email protected].5Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, P.R. China. [email protected].

AbstractRecently, hepatitis B core-related antigen (HBcrAg) has been suggested as an additional marker of hepatitis B virus (HBV) infection. This study aimed to investigate whether serum quantitative HBcrAg (qHBcrAg) was a satisfactory surrogate marker of intrahepatic covalently closed circular DNA (cccDNA). A total of 139 patients with liver biopsy were enrolled, consisting of 59 patients in immune tolerance (IT) phase, 52 patients in immune clearance (IC) phase, 18 patients in low-replication (LR) phase, and 10 patients in reactivation phase. All patients in IC phase have received entecavir (ETV) therapy, and 32 of them undergone a second liver biopsy at 24 months. Among those patients, qHBcrAg was strongly correlated with intrahepatic cccDNA, which is superior to that of qHBsAg and HBV DNA. And similar findings were also observed in patients in IT, IC, LR and reactivation phases. Among the 32 ETV-treated patients with a second liver biopsy in IC phase, the decline of intrahepatic cccDNA was accompanied by changes in both qHBcrAg and qHBsAg. However, as compared to qHBsAg, the change of qHBcrAg was more strongly associated with intrahepatic cccDNA-decline. In summary, serum qHBcrAg should be a satisfactory surrogate of intrahepatic HBV cccDNA in CHB patients.


PMID:28282964DOI:10.1038/s41598-017-00111-0

StephenW

Sci Rep。2017 Dec; 7（1）：173。 doi：10.1038 / s41598-017-00111-0。 2017年3月14日。
血清乙型肝炎核心相关抗原是肝内共价闭合环形DNA在慢性乙型肝炎的令人满意的替代标志物。
Chen EQ1,2，Feng S3，Wang ML1,2，Liang LB1,2，Zhou LY1,2，Du LY1,2，Yan LB1,2，Tao CM3，Tang H4,5。
作者信息

1
    传染病中心，四川大学华西医院，成都，中国。
2
    传染病科，四川大学生物治疗国家重点实验室，成都，中国。
3
    四川大学华西医院实验医学系，成都，中国。
4
    传染病中心，四川大学华西医院，成都，中国。 [email protected]。
5
    传染病科，四川大学生物治疗国家重点实验室，成都，中国。 [email protected]。

抽象

最近，乙型肝炎核心相关抗原（HBcrAg）已被建议作为乙型肝炎病毒（HBV）感染的额外标记。本研究旨在探讨血清定量HBcrAg（qHBcrAg）是否为肝内共价闭合环状DNA（cccDNA）的令人满意的替代标志物。总共139名肝活检患者，包括59名免疫耐受（IT）阶段，52名免疫清除（IC）阶段，18名患者在低复制（LR）阶段和10名重新激活阶段。所有IC期的患者均接受恩替卡韦（ETV）治疗，其中32例在24个月时接受第二次肝活检。在这些患者中，qHBcrAg与肝内cccDNA强相关，优于qHBsAg和HBV DNA。在IT，IC，LR和再激活阶段的患者中也观察到类似的发现。在ICC期第二次肝活检的32例ETV治疗的患者中，肝内cccDNA的下降伴随着qHBcrAg和qHBsAg的变化。然而，与qHBsAg相比，qHBcrAg的变化更强烈地与肝内cccDNA下降相关。总之，血清qHBcrAg应该是CHB患者肝内HBV cccDNA的令人满意的替代物。

PMID：
    28282964
DOI：
    10.1038 / s41598-017-00111-0