血清维生素D水平对慢性乙型肝炎患者的细胞免疫和抗病毒效

StephenW

Clin Lab. 2016 Oct 1;62(10):1933-1939. doi: 10.7754/Clin.Lab.2016.160210.
Effect of Serum Vitamin D Levels on Cellular Immunity and Antiviral Effects in Chronic Hepatitis B Patients.
He LJ, Zhang HP, Li HJ, Wang J, Chang DD.
Abstract
BACKGROUND:

To study the effect of 25-hydroxyl vitamin D3 on peripheral blood T lymphocyte immune function and antiviral effects in chronic hepatitis B patients.
METHODS:

The clinical data for 70 patients with chronic hepatitis B were analyzed. Serum 25-hydroxyl vitamin D was determined by electrochemical luminescence, and hepatitis B virus serological markers were determined by fluorescence quantitative polymerase chain reaction. Subsets of T lymphocytes were determined by immune fluorescence labeling method. These patients were divided into three groups based on serum 25-hydroxyl vitamin D level. After six months of pegylated interferon treatment, three groups have their number of T lymphocyte, liver functions, and virological indexes examined at the corresponding time.
RESULTS:

The years and ratio of gender have no statistical differences in these three groups. The proportion of CD3+, CD4+ T lymphocytes and the ratio of CD4+/CD8+ significantly increased (p < 0.05) as the level of 25-hydroxyl vitamin D increased, but the proportion of CD8+ decreased. Interferon treatment can improve the T cells subgroup, and the high level group of serum 25-hydroxyl vitamin D improved more obviously. The positive ratio of HBeAg, HBsAg and the titer of HBV DNA decreased with the increase of serum vitamin D, and the difference between the high and low level 25-hydroxyl vitamin D groups was significant (p < 0.05). The treatment of interferon can obviously improve the hepatitis B virus serological markers; the high level group of serum 25-hydroxyl vitamin D can obtain better virological response. However, there was no significant difference between the three groups of serological markers of liver function.
CONCLUSIONS:

Vitamin D may play a part in the immunologic function adjustment and immune tolerance in the natural course of chronic HBV infection, and high levels of vitamin D may be able to achieve sustained virological response. These findings may shed light on the research and treatment of chronic hepatitis B pathogenesis.

PMID:
    28164523

StephenW

临床实验室。 2016 Oct 1; 62（10）：1933-1939。 doi：10.7754 / Clin.Lab.2016.160210。
血清维生素D水平对慢性乙型肝炎患者的细胞免疫和抗病毒效果的影响。
何志杰，张惠，李浩，王杰，常德。
抽象
背景：

研究25-羟基维生素D3对慢性乙型肝炎患者外周血T淋巴细胞免疫功能和抗病毒作用的影响。
方法：

对70例慢性乙型肝炎患者的临床资料进行分析。通过电化学发光测定血清25-羟基维生素D，通过荧光定量聚合酶链反应测定乙型肝炎病毒血清学标志物。通过免疫荧光标记法测定T淋巴细胞亚群。基于血清25-羟基维生素D水平将这些患者分成三组。六个月的聚乙二醇干扰素治疗后，三组在相应的时间检查其T淋巴细胞数，肝功能和病毒学指标。
结果：

在这三组中，性别的年龄和比例没有统计学差异。随着25-羟基维生素D水平的增加，CD3 +，CD4 + T淋巴细胞的比例和CD4 + / CD8 +的比例显着增加（p <0.05），但CD8 +的比例下降。干扰素治疗可以改善T细胞亚群，高水平组血清25-羟基维生素D改善更明显。 HBeAg，HBsAg的阳性率和HBV DNA的滴度随着血清维生素D的增加而降低，高低水平的25-羟基维生素D组之间的差异显着（p <0.05）。干扰素的治疗可明显改善乙型肝炎病毒血清学标志物;高水平组的血清25-羟基维生素D可获得更好的病毒学应答。然而，三组肝功能血清学标志物之间没有显着差异。
结论：

维生素D可能在慢性HBV感染的自然过程中在免疫功能调节和免疫耐受中起作用，并且高水平的维生素D可能能够实现持续的病毒学应答。这些研究结果可能揭示慢性乙型肝炎发病机制的研究和治疗。

PMID：
    28164523