[乙型肝炎感染中非典型血清学概况：HBsAg和抗HBs同时阳性的S

StephenW

Mikrobiyol Bul. 2016 Oct; 50 (4): 535-543.
[Atypical serological profiles in hepatitis B infections: investigation of S gene mutations in cases with concurrently positive for HBsAg and anti-HBs].
[Article in Turkish]
Aydın N1, Kırdar S, Uzun N, Eyigör M, Sayan M.
Author information

    1Adnan Menderes University Medical Faculty, Department of Medical Microbiology, Aydın, Turkey. [email protected].

Abstract

Hepatitis B virus (HBV) causes different clinical manifestations, ranging from asymptomatic carriage to fulminant or chronic hepatitis. Serological tests are widely used for the diagnosis of HBV infections to detect viral markers. However, facing with atypical serological profiles in some patients leads to problems In interpreting the results of this study were this study were to investigate the atypical serologic profiles seen in patients screened for HBV infection and the S gene mutations in patients with concurrent positivity of HBsAg and anti-HBs. A total of 592 sera from patients (332 male, 260 female; age range: 13-84 years, mean age: 43.9 years) prediagnosed as HBV infection between January to September 2013, and screened for HBV markers (HBsAg, anti-HBs, HBeAg, anti -HBe, anti-HBc-IgM, anti-HBc-total and HBV-DNA) were included in the study. Of those samples 364 were screened only for HBsAg and anti-HBs markers. S gene mutations were investigated by direct sequencing method in Sera yielded atypical serologic profiles. Of these 13 cases were concurrently positive for HBsAg and anti-HBs; nine were HBeAg positive, HBeAg positive for HBsAg and anti-HBs. In our study, 5.2% (31/592) HBsAg and anti-HBe negative, anti-HBe and HBV-DNA positive. The rate of concurrent positivity of HBsAg and anti- DNA sequencing was performed for seven out of 10 samples which were positive for HBsAg, anti-HBs and anti-HBs, respectively, while 76.9% (10/13) of those cases were also positive for HBV-DNA. HBV-DNA, but three samples were not used because of the low amounts. Sequence analysis of seven samples showed S gene mutations in two samples, one was sS143L with sS193L, a HBV vaccine escape mutation, and the other was sP120R, a HBV immune HBsAg and anti-HBs; in which nine were HBV-DNA negative and anti-HBe positive, while one was positive for both HBV-DNA and anti-HBs; . The rate of concurrent positivity of HBeAg and anti-HBe was found to be 1.4% (8/592), and all of these samples were HBV-DNA positive. No single positivity for HBsAg, anti-HBc, anti- DNA was not detected in any of the patients. In conclusion, HBsAg and anti-HBs concurrent positivity was the most frequently detected atypical profile in our study (3.6%), and in some (2/7) of these patients S gene mutations were Determined.

PMID:
    28124958

StephenW

Mikrobiyol Bul。 2016 Oct; 50（4）：535-543。
[乙型肝炎感染中非典型血清学概况：HBsAg和抗HBs同时阳性的S基因突变的研究。
[土耳其文]
AydınN1，KırdarS，Uzun N，EyigörM，Sayan M.
作者信息

1 Adnan Menderes大学医学院，医学微生物学系，Aydın，土耳其。 [email protected]。

抽象

乙型肝炎病毒（HBV）引起不同的临床表现，从无症状携带到暴发性或慢性肝炎。血清学测试广泛用于诊断HBV感染以检测病毒标志物。然而，在一些患者中面临非典型的血清学概况导致问题。在解释本研究的结果时，本研究是为了研究在筛选HBV感染的患者中观察到的非典型血清学概况和在HBsAg同时阳性患者中的S基因突变抗HBs。来自2013年1月至9月期间作为HBV感染的病人（332名男性，260名女性，年龄范围：13-84岁，平均年龄：43.9岁）的592份血清，并筛选HBV标记（HBsAg，抗HBs， HBeAg，抗-HBe，抗HBc-IgM，抗HBc总和HBV-DNA）包括在研究中。在那些样品中，仅对HBsAg和抗HBs标记物筛选364。通过直接测序法在血清中研究S基因突变产生非典型血清型。在这13例中HBsAg和抗HBs阳性; 9例为HBeAg阳性，HBeAg阳性为HBsAg和抗HBs。在我们的研究中，5.2％（31/592）HBsAg和抗HBe阴性，抗HBe和HBV-DNA阳性。对于HBsAg，抗HBs和抗HBs阳性的10个样品中的7个，分别进行HBsAg和抗DNA测序的并发阳性率，而76.9％（10/13）的样品也是阳性的用于HBV-DNA。 HBV-DNA，但三个样品未使用，因为低量。七个样品的序列分析显示两个样品中的S基因突变，一个是sS143L，sS193L是HBV疫苗逃逸突变，另一个是sP120R，HBV免疫HBsAg和抗HBs;其中9例HBV-DNA阴性和抗HBe阳性，1例HBV DNA和抗HBs阳性; 。 HBeAg和抗HBe的并发阳性率为1.4％（8/592），所有这些样品均为HBV-DNA阳性。在任何患者中没有检测到HBsAg，抗HBc，抗DNA的单一阳性。总之，在我们的研究中，HBsAg和抗HBs并发阳性是最常见的非典型特征（3.6％），在一些（2/7）的这些患者中，S基因突变是确定的。

PMID：
28124958