源自不同乙型肝炎病毒基因型的乙型肝炎病毒表面抗原的比

StephenW

Virology. 2016 Dec 9;502:1-12. doi: 10.1016/j.virol.2016.12.003. [Epub ahead of print]
Comparative characterization of hepatitis B virus surface antigen derived from different hepatitis B virus genotypes.Hassemer M1, Finkernagel M1, Peiffer KH2, Glebe D3, Akhras S1, Reuter A4, Scheiblauer H5, Sommer L2, Chudy M1, Nübling CM1, Hildt E6.
Author information

• 1Paul-Ehrlich-Institut, Department of Virology, Langen, Germany.
• 2University Hospital Frankfurt, Frankfurt/Main, Germany; Goethe University Frankfurt, Frankfurt/Main, Germany.
• 3Justus Liebig University, Institute of Medical Virology, Gießen, Germany; German Center for Infection Research (DZIF), Gießen-Marburg-Langen, Germany.
• 4Paul-Ehrlich-Institut, Department of Allergology, Langen, Germany.
• 5Paul-Ehrlich-Institut, in-vitro-diagnostics, Langen, Germany.
• 6Paul-Ehrlich-Institut, Department of Virology, Langen, Germany; German Center for Infection Research (DZIF), Gießen-Marburg-Langen, Germany. Electronic address: [email protected].
  

AbstractFor human hepatitis B virus eight distinct and two candidate genotypes are described. These genotypes differ with respect to geographic distribution, molecular virology and virus-associated pathogenesis. Comparative analysis of HBV genotypes revealed, with exception of HBV/G that shows impaired HBsAg release, that no fundamental disparities between genotypes exist regarding glycosylation, subcellular distribution, release of HBsAg and formation of subviral particles. However, there are distinctions regarding the proportion of L to M to S HBs proteins detected intra- and extracellularly for different genotypes. 2D electrophoresis revealed different posttranslational modification patterns for LHBs. In light of the relevance of HBsAg as diagnostic marker, detectability of purified recombinant HBsAg of various genotypes by HBsAg-specific detection systems licensed in Europe was investigated, showing similar sensitivities for genotypes included in this analysis. These data indicate that recombinant HBsAg reproducibly purified following a defined protocol might be used as an alternative to reference materials currently established.

Copyright © 2016 Elsevier Inc. All rights reserved.

KEYWORDS: Genotypes; HBsAg; Hepatitis B virus; Surface protein

PMID:27951436DOI:10.1016/j.virol.2016.12.003

StephenW

病毒学。 2016 Dec 9; 502：1-12。 doi：10.1016 / j.virol.2016.12.003。 [打印前的电子版]
源自不同乙型肝炎病毒基因型的乙型肝炎病毒表面抗原的比较表征。
Hassemer M1，Finkernagel M1，Peiffer KH2，Glebe D3，Akhras S1，Reuter A4，Scheiblauer H5，Sommer L2，Chudy M1，NüblingCM1，Hildt E6。
作者信息

    1Paul-Ehrlich-Institut，Department of Virology，Langen，Germany。
    2大学医院法兰克福，法兰克福/德国;歌德大学法兰克福，法兰克福/ Main，德国。
    3 Justus Liebig大学，医学病毒学研究所，Gießen，德国;德国感染研究中心（DZIF），Gießen-Marburg-Langen，德国。
    4Paul-Ehrlich-Institut，Department of Allergology，Langen，Germany。
    5Paul-Ehrlich-Institut，in-vitro-diagnostics，Langen，Germany。
    6Paul-Ehrlich-Institut，Department of Virology，Langen，Germany;德国感染研究中心（DZIF），Gießen-Marburg-Langen，德国。电子地址：[email protected]。

抽象

对于人乙型肝炎病毒，描述了八种不同的和两种候选基因型。这些基因型在地理分布，分子病毒学和病毒相关发病机理方面不同。 HBV基因型的比较分析显示，除了显示受损的HBsAg释放的HBV / G之外，在基因型之间不存在关于糖基化，亚细胞分布，HBsAg释放和亚病毒颗粒形成的基本差异。然而，对于不同基因型在细胞内和细胞外检测的L对M的比例与S HBs蛋白的比例有区别。 2D电泳显示不同的LHBs的翻译后修饰模式。鉴于HBsAg作为诊断标记物的相关性，通过在欧洲许可的HBsAg特异性检测系统检测多种基因型的纯化的重组HBsAg的可检测性，对包括在该分析中的基因型显示类似的敏感性。这些数据表明，按照确定的方案可重复纯化的重组HBsAg可用作目前建立的参考物质的替代物。

版权所有©2016 Elsevier Inc.保留所有权利。
关键词：

基因型; HBsAg;乙型肝炎病毒;表面蛋白

PMID：
    27951436
DOI：
    10.1016 / j.virol.2016.12.003