Hepatitis B Virus Infection of a Mouse Hepatic Cell Line Reconstituted with Human Sodium Taurocholate Cotransporting Polypeptide - aDepartment of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany
- bKey Laboratory of Medical Molecular Virology, Institute of Medical Microbiology, Shanghai Medical College, Fudan University, Shanghai, China
- cGerman Center for Infection Research (DZIF), Heidelberg, Germany
ABSTRACT Hepatitis B virus (HBV) enters hepatocytes via its receptor, human sodium taurocholate cotransporting polypeptide (hNTCP). So far, HBV infection has been achieved only in human hepatic cells reconstituted with hNTCP and not in cells of mouse origin. Here, the first mouse liver cell line (AML12) which gains susceptibility to HBV upon hNTCP expression is described. Thus, HBV infection of receptor-expressing mouse hepatocytes does not principally require a human cofactor but can be triggered by endogenous murine determinants.
FOOTNOTES
- Received 11 November 2015.
- Accepted 5 February 2016.
- Accepted manuscript posted online 10 February 2016.
- Address correspondence to Stephan Urban, Stephan.Urban{at}med.uni-heidelberg.de.
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Citation Lempp FA, Qu B, Wang Y-X, Urban S. 2016. Hepatitis B virus infection of a mouse hepatic cell line reconstituted with human sodium taurocholate cotransporting polypeptide. J Virol 90:4827–4831. doi:10.1128/JVI.02832-15.
- Copyright © 2016, American Society for Microbiology. All Rights Reserved.
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